歯根形成初期臼歯歯胚のヘルトビッヒ上皮鞘に及ぼす成長因子の影響

生长因子对早期牙根形成过程中磨牙牙胚赫特维希上皮鞘的影响

• 批准号: 13877309
• 负责人: 藤原 尚樹
• 金额: $ 1.09万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Exploratory Research
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13877309/
• 关键词: 歯根形成; 臼歯歯胚; insulin-like growth factor I; ヘルトビッヒ上皮鞘; 器官培養; マウス; insulin-like growth factor

昨年度我々はマウス臼歯歯胚歯根形成初期においてIGF-Iが外エナメル上皮の細胞増殖を促進することでヘルトビッヒ上皮鞘(HERS)の分化調節に関わることを示唆する結果を得た。今年度はさらにHERS細胞の動態と他の因子の作用について検討した。1,成侵に伴うHERS細胞の増殖とその変化を確認するために生後5、10、15、20日のマウスにおいて腹腔内にBrdUを投与し、経時的にマウスから下顎第一臼歯歯胚を摘出・固定し、HERS細胞へのBrdU取込み数の変化を調べた。歯根形成開始期(生後5日)でBrdU陽性細胞が多く見られ、また外エナメル上皮の陽性細胞数が、時局経過ともにしだいに減少する傾向がみられた。このことはHERSの外エナメル上皮細胞が盛んに分裂を繰り返すことを意味すると考えている。2,局所的に外因性因子をデリバリーするヘパリンbeadsを用いた特殊な器官培養法を習得する機会に恵まれ、IGF-Iをしみ込ませたbeadsを生後5日齢の臼歯ヘルトビッヒ上皮鞘付近に置き、4日間培養を行った。Beadsを置いた実験群は対照群に比べヘルトビッヒ上皮鞘の伸長が顕著であることが確認できた。このことは培養液にIGF-Iを添加して行った昨年度の実験結果をより明確にした。3,IGF-I以外の外因性因子のHERSの発達に及ぼす影響を検討した。PDGFはヘルトビッヒ上皮鞘の伸長を促進する効果を、EGFは歯頚彎曲部のエナメル上皮からヘルトビッヒ上皮鞘への分化を抑制する効果が見られた。この時期の臼歯歯胚歯頚部に発現するEGFをRT-PCRで確認すると、成長と共に減弱する傾向がみられたことから、EGFもヘルトビッヒ上皮鞘の発達に何らかの影響を持つことが示唆された。

In the early stage of root formation, we used IGF-I cells to promote the differentiation of epithelium sheath (HERS) in the early stage of root formation. This year, the HERS cell dynamics and other factors play an important role. 1. The colonization of HERS cells with invasion confirmed that BrdU was injected into the abdominal cavity on the 5th, 10th, 15th and 20th day after birth, and the first molluscum embryos were removed and fixed, and HERS cells were harvested by BrdU on the 5th, 10th, 15th and 20th after birth. At the beginning of root formation (5 days after birth), the number of BrdU abnormal cells was increased, the number of epithelial cells was low, and the number of abnormal cells was reduced. It means that the epithelial cells are in full bloom, the cells are divided, the epithelial cells are divided, and the epithelial cells are divided. 2. The external factor of the bureau was that the beads was tested by special organ culture method, and the beads was tested by special organ culture. The epithelia sheath was closed in 5 days after birth, and cultured in 4 days after birth. The epithelial sheath of Beads is longer than that of the control group, and the epithelial sheath is elongated. Please add a new line to the IGF-I system. The results of last year's performance show that the results are very clear. (3) the external factors other than HERS color I have reached the level of the external factors and affected the quality of the patients. The elongation of the epithelial sheath of the PDGF gene promoted the growth of the fruit, and the differentiation of the epithelial sheath of the EGF was inhibited. During the period of time, the RT-PCR department of the acetabular tube makes sure that the growth rate is low and the growth rate is weak. The epithelial sheath of the acetabular tissue and the EGF tissue are sensitive to the quality of the epithelial sheath, so as to show that the epithelial sheath is safe and sound.