Investigation of the spatial distribution of OXPHOS complex assembly in mammalian cells

哺乳动物细胞中 OXPHOS 复合体组装空间分布的研究

• 批准号: 426882322
• 负责人: Professor Dr. Stefan Jakobs
• 金额: --
• 依托单位: Institut für Zellbiochemie
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/426882322?language=en
• 关键词: Investigation; spatial; distribution; OXPHOS; complex

The mitochondrial oxidative phosphorylation system (OXPHOS) is critical for cellular ATP production. Four protein complexes of the electron transport chain generate the proton gradient across the inner membrane that drives the F1Fo-ATP Synthase. The OXPHOS complexes consist of nuclear- and mitochondrial-encoded subunits. Accordingly, it assembles from imported subunits and those that are translated in mitochondria. The biogenesis process is thought to progress through transient assembly intermediates. The imported subunits are inserted into the inner membrane at the inner boundary membrane by protein translocases. However, the topology of subsequent stages of the assembly process are unknown. In this project, we will define the spatial organization and topological heterogeneity of OXPHOS assembly in mammalian cells and investigate how it is linked to gene expression and quality control processes. Furthermore, we will define their mitochondrial localization with nanoscale resolution.

线粒体氧化磷酸化系统（OXPHOS）对细胞ATP的产生至关重要。电子传递链的四种蛋白质复合物产生穿过内膜的质子梯度，驱动F1 Fo-ATP合酶。OXPHOS复合物由核编码亚基和核蛋白编码亚基组成。因此，它由输入的亚基和在线粒体中翻译的亚基组装而成。生物发生过程被认为是通过短暂的组装中间体进行的。导入的亚基通过蛋白质移位酶插入内膜的内界膜。然而，组装过程的后续阶段的拓扑结构是未知的。在这个项目中，我们将定义OXPHOS组装在哺乳动物细胞中的空间组织和拓扑异质性，并研究它是如何与基因表达和质量控制过程相关联的。此外，我们将定义其线粒体定位与纳米级分辨率。