Investigation of the spatial distribution of OXPHOS complex assembly in mammalian cells

线粒体氧化磷酸化系统（OXPHOS）对细胞ATP的产生至关重要。电子传递链的四种蛋白质复合物产生质子梯度穿过内膜，驱动F1Fo-ATP合酶。OXPHOS复合物由核和线粒体编码的亚基组成。因此，它从输入的亚基和线粒体中翻译的亚基组装。生物发生过程被认为是通过瞬时组装中间体进行的。输入的亚基通过蛋白质转位在内边界膜插入到内膜中。然而，装配过程的后续阶段的拓扑结构是未知的。在这个项目中，我们将定义OXPHOS组装在哺乳动物细胞中的空间组织和拓扑异质性，并研究它如何与基因表达和质量控制过程相关联。此外，我们将以纳米级分辨率定义它们的线粒体定位。

The mitochondrial oxidative phosphorylation system (OXPHOS) is critical for cellular ATP production. Four protein complexes of the electron transport chain generate the proton gradient across the inner membrane that drives the F1Fo-ATP Synthase. The OXPHOS complexes consist of nuclear- and mitochondrial-encoded subunits. Accordingly, it assembles from imported subunits and those that are translated in mitochondria. The biogenesis process is thought to progress through transient assembly intermediates. The imported subunits are inserted into the inner membrane at the inner boundary membrane by protein translocases. However, the topology of subsequent stages of the assembly process are unknown. In this project, we will define the spatial organization and topological heterogeneity of OXPHOS assembly in mammalian cells and investigate how it is linked to gene expression and quality control processes. Furthermore, we will define their mitochondrial localization with nanoscale resolution.