Atomic force microscope integrated with an inverted optical microscope

与倒置光学显微镜集成的原子力显微镜

• 批准号: 427377229
• 金额: --
• 依托单位: Zentrum für Medizinische Biotechnologie (ZMB)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/427377229?language=en
• 关键词: Atomic; force; microscope; integrated; inverted

Coupled atomic force/fluorescence microscopy offers extraordinary possibilities to analyze biological processes at a high spatial and temporal resolution. Such a modern technique is at the moment not available within the University Duisburg-Essen (UDE) and its establishment is not only desirable but even necessary for the development of high-quality research projects. Currently, there is only one atomic force microscope available in the Faculty of Biology and accessible to other groups of the UDE. Although of excellent performance for topographical characterization of nanostructures, this instrument is not suitable for analysis of larger biological samples, such as cells, and its low temporal resolution severely limits the study of biological processes in live samples and in real-time. The coupled atomic force/fluorescence microscope applied here would provide the spatial and temporal resolution necessary to satisfy this request, offering also the unique possibility to investigate both the surface and interior of cells, by coupling the topographical/force signal with an optical readout. In the past few years, the demand on high-resolution techniques at the single-cell or single-particle level has tremendously increased within the Faculty of Biology of the UDE, particularly thanks to the successful DFG-funding of the CRC initiative and the consequent establishment of challenging cooperation projects among biologists and chemists of the University Duisburg-Essen. For this reason, such an instrument will provide the technological tools necessary to face these projects in a suitable manner, with the aim of performing high-quality research.

耦合原子力/荧光显微镜提供了非凡的可能性，以高空间和时间分辨率分析生物过程。这样一种现代技术目前在杜伊斯堡-埃森大学（UDE）内是不可用的，它的建立不仅是可取的，甚至是发展高质量研究项目所必需的。目前，生物学院只有一台原子力显微镜，可供UDE的其他小组使用。尽管该仪器在纳米结构的形貌表征方面具有优异的性能，但它不适合分析较大的生物样品，如细胞，并且其低的时间分辨率严重限制了对活样品和实时生物过程的研究。这里应用的耦合原子力/荧光显微镜将提供满足这一要求所需的空间和时间分辨率，还提供了独特的可能性，通过将形貌/力信号与光学读数耦合来研究细胞的表面和内部。在过去的几年里，在单细胞或单粒子水平的高分辨率技术的需求已经大大增加了在生物学的UDE学院，特别是由于成功的DFG资助的CRC倡议和随后建立具有挑战性的合作项目之间的杜伊斯堡-埃森大学的生物学家和化学家。因此，这样一个工具将提供必要的技术工具，以适当的方式面对这些项目，目的是进行高质量的研究。