Dynamics and ultrastructure of myoblast fusion and the involvement of the APC/CFzr

成肌细胞融合的动力学和超微结构以及 APC/CFzr 的参与

• 批准号: 427816516
• 负责人: Professor Dr. Achim Paululat
• 金额: --
• 依托单位: Arbeitsgruppe Zoologie-Entwicklungsbiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/427816516?language=en
• 关键词: Dynamics; ultrastructure; myoblast; fusion; involvement

Somatic muscles of all higher organisms arise during embryogenesis by the coordinated fusion of individual myoblasts into larger, syncytial muscle fibres. In vertebrates, thousands of single myoblasts contribute to the formation of one individual muscle. During a genetic screen for mutations affecting heart and muscle development in the embryo, we identified the APC/CFzr as a novel key player during myoblast fusion. Embryos mutant for fzr exhibit an arrest of myoblast fusion, and display a massive reduction of mature muscle fibres as well as an access of unfused myoblasts by the end of embryogenesis. In this proposal, we aim to identify and understand the mechanism of the APC/CFzr on the fusion process by applying state-of-the art light- and electron microscopy as well as proteomic approaches.

所有高等生物的躯体肌肉在胚胎发生期间通过单个成肌细胞协调融合成更大的合体肌肉纤维而产生。在脊椎动物中，数以千计的单个成肌细胞对一块肌肉的形成做出了贡献。在对影响胚胎心脏和肌肉发育的突变进行基因筛选的过程中，我们发现APC/CFzR是成肌细胞融合过程中的一个新的关键角色。FZR突变的胚胎表现为成肌细胞融合停止，成熟肌纤维大量减少，胚胎发生结束时未融合的成肌细胞进入。在这项提案中，我们旨在通过应用最先进的光学和电子显微镜以及蛋白质组学方法来识别和理解APC/CFZR在融合过程中的机制。