Development of a breath test for caries detection - a pilot study.

开发用于龋齿检测的呼气测试——一项试点研究。

• 批准号: 427629778
• 负责人: Privatdozentin Dr. Saskia Preissner
• 金额: --
• 依托单位: Abteilung für Zahnerhaltung und Präventivzahnmedizin
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/427629778?language=en
• 关键词: Development; breath; test; caries; detection

Caries is one of the most common chronic diseases worldwide and the most reliable, but least favorable predictor of future cancer is still the disease progression. However, it would be desirable to develop a diagnostic tool that would be effective before the emergence of the first carious lesions requiring therapy.The aim of this clinical pilot study within the framework of the DFG Nachwuchsakademie is the development of a breath test for caries detection. Based on the data obtained in this pilot study, an instrument for early detection and risk stratification will be developed in the future.In published preliminary work on gas chromatography with coupled mass spectroscopy, specific patterns of volatile organic compounds for pathogenic microorganisms were detected and a workflow for the clinical sampling was established.The planned project aims to correlate breath samples of 25 diseased patients with those from 25 caries-free subjects and to identify specific patterns in caries patients using a deep-learning approach. By means of cross-validation, sensitivity and specificity of the breath test are determined and the learning process is optimized.We assume that it is suitable for the risk stratification and early detection of carious lesions. In this case, a simple, low-cost and risk-free point-of-care diagnostic will be developed in follow-up projects, which, especially in children, would mean that many X-rays could be avoided with and individualized prophylaxis could be introduced.

龋病是世界范围内最常见的慢性疾病之一，并且疾病进展仍然是未来癌症的最可靠但最不利的预测因素。然而，希望开发一种在需要治疗的第一个龋损出现之前有效的诊断工具。DFG Nachwuchsakademie框架内的这项临床试点研究的目的是开发用于龋齿检测的呼吸测试。根据这项初步研究所获得的数据，今后将开发一种用于早期检测和风险分层的仪器。在已发表的气相色谱-质谱联用的初步工作中，检测到病原微生物的挥发性有机化合物的特定模式，并建立了临床采样的工作流程。计划中的项目旨在将25名患病患者的呼吸样本与25名无龋受试者，并使用深度学习方法识别龋齿患者的特定模式。通过交叉验证，确定了呼吸试验的敏感性和特异性，优化了学习过程，我们认为它适用于龋损的危险分层和早期检测。在这种情况下，将在后续项目中开发一种简单、低成本和无风险的护理点诊断，这意味着可以避免许多X射线，特别是在儿童中，并可以采用个性化预防。