Development of rapid test for biomarkers in exhaled breath condensate in patients with asthma and its use for the management of asthmatics

为了评估哮喘患者的呼吸道炎症，我们开发了一种快速检测呼气冷凝液(EBC)中中性粒细胞和嗜酸性粒细胞生物标志物的护理点检测方法。用双抗体夹心法检测哮喘患者外周血中嗜酸性粒细胞标志的主要碱性蛋白(MBP)和中性粒细胞标志的髓过氧化物酶(MPO)。我们建立了免疫层析法半定量测定EBC中MPO和MBP的含量。免疫层析结果显示，哮喘患者分为嗜酸性标志物占优势组和中性粒细胞标志物占优势组。该方法可应用于哮喘的治疗。

To evaluate airway inflammation in asthmatic patients, we developed a rapid test as point of care testing detecting neutrophilic and eosinophilic biomarkers in exhaled breath condensate (EBC). Major basic protein (MBP) for eosinophilic marker and Myeloperoxidase (MPO) for neutrophilic marker were detected in EBC by ELISA methods in patients with asthma. We established immunochromatography for semi-quantification of MPO and MBP in EBC. The findings of immunochromatography indicate that asthmatic patients were divided into two groups including eosinophilic marker predominant group and neutrophilic marker predominant group. This method could be applied to the management of asthma.