Vascularized neuromuscular constructs on PCL-collagen I-PEO-nanoscaffolds

PCL-胶原蛋白 I-PEO-纳米支架上的血管化神经肌肉结构

• 批准号: 428098717
• 负责人: Dr. Aijia Cai
• 金额: --
• 依托单位: Plastisch- und Handchirurgische Klinik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/428098717?language=en
• 关键词: Vascularized; neuromuscular; constructs; PCL; collagen

Volumetric muscle loss is critical since it exceeds the natural regeneration capacity of skeletal muscle tissue.Tissue engineering of skeletal muscle could be a treatment option without having to sacrifice other functional muscles. For this purpose, suitable cells as well as an adequate matrix, representing the hierarchical and complex 3D structure of skeletal muscle, are needed. Furthermore, volumetric 3D tissue constructs necessitate adequate vascularization. For the generation of functional muscle, innervation of the neotissue is also necessary.Aim of this study is the generation of skeletal muscle tissue by co-cultivating primary myoblasts, adipose tissue-derived stem cells (ADSC), and schwann cells on aligned PCL-collagen I-nanofibers. Cells will be myogenically differentiated and the 3D constructs will be implanted in vivo into an arteriovenous (AV-) loop model with integration of a motoric nerve, the so-called EPI-loop model, to enable neurotisation. By these means, we aim to create vascularized and functional skeletal muscle tissue.

肌肉体积损失是至关重要的，因为它超过了骨骼肌组织的自然再生能力。骨骼肌组织工程可能是一种治疗选择，而不必牺牲其他功能性肌肉。为此，需要合适的细胞以及适当的基质，代表骨骼肌的层次和复杂的3D结构。此外，体积3D组织构建需要足够的血管化。对于功能性肌肉的产生，神经支配的新生tissue. The目的也是必要的，这项研究是通过共培养原代成肌细胞，脂肪组织来源的干细胞（ADSC），和雪旺细胞对齐PCL-胶原蛋白I-nanofibers的骨骼肌组织的产生。细胞将肌源性分化，3D构建体将在体内植入动静脉（AV-）环模型，其中整合了运动神经，即所谓的EPI环模型，以实现神经化。通过这些手段，我们的目标是创造血管化和功能性骨骼肌组织。