骨の力学的適応現象の解明を目指した骨組織形成過程のその場観察系の確立

建立骨组织形成过程原位观测系统，阐明骨骼的机械适应现象

• 批准号: 19650114
• 负责人: 松本 健郎
• 金额: $ 2.11万
• 依托单位: Nagoya Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Exploratory Research
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19650114/
• 关键词: バイオメカニクス; 機械材料・材料力学; 生物・生体工学; 細胞・組織; 力学的適応; その場観察

昨年度は,石灰化過程ならびにコラーゲン線維の走行を観察する方法を確立し,倒立型顕微鏡ステージ上に設置可能な培養装置を試作するとともに,鶏雛脛骨を厚さ200μmに薄切して無菌的に培養する方法の確立に成功した.そこで研究後半の本年度は,骨薄切試料に力学負荷が可能なように培養装置を改良するとともに,準静的ならびに動的な負荷を加えつつ骨試料を培養し,骨形成過程に与える力学負荷の影響を調べた.すなわち,鶏雛脛骨の骨軸方向に垂直な薄切試料の両端をバネ機構を利用してクランプし,試料面内方向に引張・圧縮を加えることのできる装置を試作した.予備的試験の結果,試料に引張を安定して加えることは可能であったが,圧縮は試料の座屈により加えることが困難であった.この装置を用いて試料に準静的な引張を24時間加えたところ,試料に加わる引張ひずみが5%以上の場合,石灰化が有意に促進されることが明かとなった.ひずみが3%以下の場合には静置培養された試料と有意差がなかった.また,石灰化の方向を調べたところ,試料の引張方向に石灰加速度が有意に早いことが明かとなった.次に,試料の引張に伴うコラーゲン線維の走行変化を偏光顕微鏡を用いて調べたところ,石灰化の進行方向はコラーゲン線維の走行方向とよい相関があること,石灰化の速度が上昇するひずみ3%前後を境として,コラーゲン線維の走行が引張り方向に揃うことなどが明らかとなった.コラーゲンの配向が幼若骨組織の石灰化を促進・誘導する可能性が示唆された.

The method of observing the lime process and the movement of the line was established yesterday. The method of setting up a possible culture device on an inverted microscope was successfully established. In the second half of this year, the mechanical load of bone thin-cut samples may be improved, and the influence of mechanical load on bone formation process may be adjusted. The mechanism for producing the end of the thin cut sample in the vertical direction of the bone axis of the tibia was used to test the device for increasing the tension and compression in the in-plane direction of the sample. The results of the preliminary test show that the sample tension is stable and the sample compression is difficult. This device is used to test the sample for static tension 24 hours, test sample for tension more than 5% of the case, lime is intentionally promoted. When the concentration is below 3%, the test sample will be incubated for a long time. The direction of lime is adjusted, and the acceleration of lime in the direction of sample tension is intentionally adjusted. Second, the tension of the sample is accompanied by a change in the direction of travel of the linear dimension, a change in the direction of travel of the polarizing microscope, a change in the direction of travel of the linear dimension, a change in the direction of travel of the lime, a change in the speed of lime, a change in the direction of travel of the linear dimension, a change in the direction of travel of the linear dimension, a change in the direction of travel of the lime, a change in the direction of travel of travel of the lime, a change in the direction of travel of travel of the lime, a change in the direction of travel of travel of the lime, a change in the direction of travel of the lime, a change in the direction of travel of travel of the lime, a change in the direction of travel of the lime, a change The possibility of promoting and inducing calcification of young bone tissue due to the alignment of the protein is demonstrated.

项目成果

Microscopic Mechanical Analysis of Aortic Wall : Estimation of Stress in the Intramural Elastic Laminas and Smooth Muscle Cells in a Physiological State

主动脉壁的显微力学分析：生理状态下壁内弹力层和平滑肌细胞的应力估计

• 发表时间: 2008
• 作者: T. Sugiura;M. Nakao;T. Sato and K. Minato;Nagayama K;Tamura A;Nagayama K;Nagayama K;松本 健郎;Matsumoto T
• 通讯作者: Matsumoto T

A Novel Micro Tensile Tester with Feed-back Control for Viscoelastic Analysis of Single Isolated Smooth Muscle Cells

一种具有反馈控制的新型微拉伸测试仪，用于单个分离平滑肌细胞的粘弹性分析

• 发表时间: 2007
• 期刊: Medical Engineeing & Physics Q9
• 作者: T. Sugiura;M. Nakao;T. Sato and K. Minato;Nagayama K;Tamura A;Nagayama K
• 通讯作者: Nagayama K

Blood vessel wall as composite materials: Mechanical analysis of aortic walls considering microscpic structure

作为复合材料的血管壁：考虑微观结构的主动脉壁力学分析

• 发表时间: 2007
• 作者: T. Sugiura;M. Nakao;T. Sato and K. Minato;Nagayama K;Tamura A;Nagayama K;Nagayama K;松本 健郎;Matsumoto T;Yang YF;Nagayama K;Matsumoto T;Matsumoto T
• 通讯作者: Matsumoto T

Contribution of actin filaments and microtubules to quasi-in situ tensile properties and internal force balance of cultured smooth muscle cells on a substrate

• DOI: 10.1152/ajpcell.00098.2008
• 发表时间: 2008-12-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
• 影响因子: 5.5
• 作者: Nagayama, Kazuaki;Matsumoto, Takeo
• 通讯作者: Matsumoto, Takeo

On the Intracellullar Memory of Stress Fiber Orientation : Effects of Microtubules and Focal Adhesions on the Repolymerization Process of Stress Fibers

关于应力纤维取向的细胞内记忆:微管和局部粘附对应力纤维再聚合过程的影响

• 发表时间: 2008
• 作者: T. Sugiura;M. Nakao;T. Sato and K. Minato;Nagayama K;Tamura A;Nagayama K;Nagayama K;松本 健郎;Matsumoto T;Yang YF
• 通讯作者: Yang YF