高血圧に伴う血管壁のリモデリング機構解明のための摘出血管の人工培養システム

离体血管人工培养系统阐明高血压相关血管壁重塑机制

• 批准号: 06213206
• 负责人: 松本 健郎
• 金额: $ 2.5万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06213206/
• 关键词: 生体軟組織; 力学的適応; 血管; 器官培養; 平滑筋; バイオメカニクス

【方法】昨年度開発した血管培養システムを用いて血管培養を試みた.無菌的に摘出した家兎頸動脈を灌流培養,無負荷培養,対照の3群に分けた.灌流培養群では試料を装置に取付けて内圧100mmHg,壁剪断応力約10dyne/cm^2の条件で3日間培養した.培養液にはDMEM+20%FCSを用いた.無負荷培養群では試料を培養槽内に静置した.両群とも培養終了時に内圧負荷試験を行い内圧-外径関係を求めた.次に10^5Mのノルアドレナリンで平滑筋を収縮させた時の内圧-外径関係を求めた.平滑筋収縮前後の同一内圧における外径を比較して,内圧-外径収縮量関係を求め,平滑筋活性の指標とした.実験終了後,血管を輪切りにして実体顕微鏡下で無負荷時の内外径,全壁厚,中膜壁厚を求めた.対照群では摘出後6時間以内に同様の実験を行った.【結果】内圧-外径曲線には灌流培養群-対照群間では有意差がなかったが,無負荷培養群では100mmHg以上の外径が対照群に比べ有意に小さく,曲線の傾きが急峻になる内圧も有意に低下していた.内圧-収縮量曲線に関しては収縮の最大値には3群間で有意差がなかったが,生理的内圧(100mmHg)での収縮量は対照群に比べ両培養群で有意に低下していた.また無負荷時の内外径・全壁厚は3群で有意差がなかったが,中膜壁厚は無負荷培養群で対照群に比べて有意に薄かった.【考察】家兎頸動脈は壁厚が100μm程度と薄いため,壁内外からの拡散栄養で活性を維持できるのではないかと予想した.ところが生理的な力学条件下で血管を培養しても,血管の収縮特性には変化が現れた.一方,無負荷状態で培養すると,収縮特性だけでなく内圧-外径関係や中膜壁厚にも変化が現れた.このことは,血管の形態や機能を維持するためには何らかの力学的負荷が必要なことを意味すると考えられる.

[Methods] The vascular culture system was developed yesterday. Aseptic extraction of the isolated carotid artery perfusion culture, non-load culture, three groups of light separation. Perfusion cultures were incubated for 3 days at an internal pressure of 100mmHg and a wall shear force of about 10dyne/cm^2. The culture medium is prepared from DMEM+20% FCS. No load culture group, no sample culture tank, no static. At the end of the incubation period, the internal pressure load test was carried out to determine the internal pressure-external diameter relationship. The relationship between internal pressure and external diameter is calculated when the smooth rib is compressed. Comparison of the same internal pressure and external diameter before and after smooth tendon contraction, relationship between internal pressure and external diameter contraction, smooth tendon activity index. The diameter, wall thickness and media wall thickness of the vessel under microscope were calculated. The same thing happened within 6 hours after the group was picked up. [Results] The curve of internal pressure versus external diameter was intentionally different between perfusion culture group and contrast group, and the curve of external diameter above 100mmHg was intentionally smaller than contrast group, and the curve was more acute than contrast group. The maximum value of the internal pressure-contraction curve is the intentional difference between the three groups. The physiological internal pressure (100mmHg) is the intentional decrease in the contraction of the contrast group compared with the culture group. The inner and outer diameters of the three groups are intentionally different under no load, and the inner and outer diameters of the three groups are intentionally thinner than those of the three groups under no load. [Investigation] The wall thickness of the carotid artery in the home is 100μm, and the activity of the internal and external walls is maintained. Under physiological and mechanical conditions, blood vessels are cultured and their contraction characteristics are revealed. On the one hand, the relationship between inner pressure and outer diameter and the change of membrane wall thickness are found in the condition of no load. The shape and function of blood vessels are maintained, and the mechanical load is necessary.

项目成果

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Takeo Matsumoto: "Mechanical and Dimensional Adaptation of Rat Aorta to Hypertension" ASME,Journal of Biomechanical Engineering. 116. 278-283 (1994)

Takeo Matsumoto：“大鼠主动脉对高血压的机械和尺寸适应”ASME，生物机械工程学杂志。

Noriyuki Kataoka: "Application of the Pipette Aspiration Technique to the Measurement of Local Elastic Moduli of Cholesterol-Fed Rabbt Aortas" Theoretical and Applied Mechanics. 43. 233-238 (1994)

Noriyuki Kataoka：“移液管抽吸技术在测量胆固醇喂养的兔子主动脉局部弹性模量中的应用”理论和应用力学。

Kozaburo Hayashi: "Clinical Biomechanics and Related Research" Biomechanical Response of Aortic Wall to Hypertension in the Rat, (1994)

Kozaburo Hayashi：“临床生物力学及相关研究”大鼠主动脉壁对高血压的生物力学反应，（1994）