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Functionality of Bovive Milk Fat Globule Membrane

牛乳脂肪球膜的功能

基本信息

批准号：
01470132
负责人：
KANNO Choemonn
金额：
$ 3.58万
依托单位：
Utsunomiya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

We studied on the functionality of bovine milk fat globule membrane (MFGM) with special reference to 1) the emusifying ability of MFGM in milk fat globules (MFG), 2) the acceleration of intestinal absorption of riboflavin (RF) by MFGM and 3) inhibition of lipolysis due to lipoprotein lipase (LPL) in milk.1) MFG emulsions were reconstituted by homogenizing 25% milk fat and 1% MFGM in 0.01M phosphate buffer (pH 7.0) for 1 min at 19 k rpm and at 45^0C. Maximal emulsifying properties were attained at 25% milk fat, 1% MFGM, neutral pH and the increased amount of MFGM. The major variables influencing the reconstitution of MFG were emulsifying time, MFGM and milk fat contents, and pH. Viscosity was highest at pH 5.0 and at 4 min of emulsifying time, and increased with MFGM content. The composition of MFGM protein adsorbed on MFG was not affected by the major variables. However, the amount of adsorbed protein increased at acidic pH and with the increased amount of MFGM. Diameter of MFG was 0.9 … More -17.4mum and median diameter was 5.0mum and its specific surface area was 15.6 m^2/m^3.2) Riboflavin bound MFGM was administered (3mug/g of diet) TO RF-defficient rats in who glutathion reductase activity ration (EGRAR, +FAD/-FAD) was 3.5, in comparison with free RF, after RF deprivation for 4 weeks. After RF feeding, EGRAR recovered to 1.6-1.9 and body weights increased in the fifth weeks. Gain of body weights for 1 week in the fifth weeks was 50.2<plus-minus>3.8 g for MFGM-RF-fed group, 40.4<plus-minus>3.9 g for free RF-fed group, and 33.1<plus-minus>5.4 g for pair-fed group. Significant differences were found among 3 groups (P<0.0 1). MFGM seemed to accelerate the absorption of RF from the intestine of rats. The peptides from MFGM may act as a signal peptide for the acceleration of the incorporating RF from intestinal mem3) Lactophrin (LP), which is immunologically identical to the soluble glycoprotein (SGP) of MFGM, was isolated from milk whey. LP added in fresh bovine milk sonicated for 2 min at 200 W inhibited the lipolysis induced by milk indigeneus LPL. The ratio of inhibition was about 62-67% for LP and 30-43% for the LP- enriched component-3 fraction of proteose-peptone at 40 mg of protein/ml of milk over the control. LP and SGP seemed to be inhibited the lipolytic action of LPL by a high ability to replenish the surface of MFG. Less

本文对牛乳脂肪球膜（MFGM）的功能性进行了研究，主要包括：1）MFGM对乳脂球（MFG）的乳化能力; 2）MFGM促进核黄素（RF）在肠道的吸收; 3）抑制乳中脂蛋白脂酶（LPL）引起的脂解作用。1）MFG乳剂由100%的乳脂和1%的脂肪组成。MFGM在0.01 M磷酸盐缓冲液（pH 7.0）中以19 k rpm和45 ℃下搅拌1 min。在25%乳脂、1%MFGM、中性pH和增加MFGM用量的条件下，获得最大乳化性能。影响MFG重构的主要变量是乳化时间、MFGM和乳脂含量以及pH。粘度在pH 5.0和乳化时间4 min时最高，并随MFGM含量增加而增加。吸附在MFG上的MFGM蛋白的组成不受主要变量的影响。然而，吸附的蛋白质的量增加，在酸性pH值和MFGM量的增加。MFG直径为0.9 ...更多信息 -17.4 μ m，中位直径为5.0 μ m，比表面积为15.6 m^2/m^3。2）在RF剥夺4周后，将核黄素结合的MFGM（3 μ g/g饮食）给予RF缺乏的大鼠，其中与游离RF相比，谷胱甘肽还原酶活性比（EGRAR，+FAD/-FAD）为3.5。RF喂养后，EGRAR恢复到1.6-1.9，体重在第5周增加。在第五周中，MFGM-RF喂养组的1周体重增加为50.2 ± <plus-minus>3.8 g，<plus-minus>游离RF喂养组为40.4 ± 3.9 g，<plus-minus>成对喂养组为33.1 ± 5.4 g。3组间差异有显著性（P<0.01）。MFGM能促进大鼠小肠对RF的吸收。MFGM中的肽段可能是促进肠膜RF掺入的信号肽。3）从乳清中分离到与MFGM的可溶性糖蛋白（SGP）免疫学上相同的乳蛋白（LP）。在200 W超声处理2 min的鲜牛乳中加入LP，可抑制乳原LPL诱导的脂肪分解。与对照相比，在40 mg蛋白质/ml牛奶下，LP的抑制率约为62-67%，蛋白酶-蛋白胨的LP富集组分-3级分的抑制率约为30-43%。LP和SGP对MFG表面的高补充能力似乎抑制了LPL的脂解作用。少