Significance of Crude Drug Composition and Plural Components in Kampo Prescription.

汉方处方中生药成分和复数成分的意义。

• 批准号: 01470140
• 负责人: OGIHARA Yukio
• 金额: $ 3.58万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01470140/
• 关键词: Kampo prescription; Shosaikoto; Daisaikoto; Macrophage; Interleukin-1; Saikosaponin; Hemolytic action; Phagocytosis; グリチルリチン; 複合成分; 生薬配合; 大柴胡夜; サポニン; 薬理作用; コルチコステロン

Using Shosaikoto and Daisaikoto which consists of crude drugs similar to Shosaikoto in order to investigate the significance of crude drug composition and plural components in kampo prescriptions, we made clear the facts described below.1. Shosaikoto might regulate the immune response by reducing prostaglandin production in macrophages and stimulating interleukin-1 release and phagocytosis.2. Stimulation of interleukin-1 release by Shosaikoto resulted from the reduction of pro-staglandin production and other unknown mechanisms.3. Shosaikoto and Daisaikoto showed the opposite action on interleukin-1 release, which was explained by the action of the crude drugs peculiar to Shosaikoto and Daisaikoto, respectively, and by the subsidiary action of the crude drugs common to them.4. Shosaikoto reduced monocytes/macrophages in blood with the increase inserum choles-terol in hyperlipidemic rabbit. On the contrary, Daisaikoto maintained the same level as control rabbit.5. Using components of Sho … More saikoto which were fractionated by solubility in ethanol and molecular size, the stimulatory action of Shosaikoto on phagocytosis of macrophages was studied. Ethanol insoluble fraction and ethanol soluble fraction of which molecular weight was smaller than 1000, showed as much stimulatory activity of phagocytosis as Shosaikoto or more than it.6. The study of the variation in component quantity by changing the composition of crude drugs of Shosaikoto indicated that Scutellariae Radix, Zizyphi Fructus, Bupleuri Radix and Ginseng Radix stimulated the extraction of glycyrrhizin from Glycyrrhizae Radix and that Zingiberis Rhizomas and Pinelliae Tuber reduced it, And this reduction resulted from the absorption of glycyrrhizin to the residues of crude drugs.7. Anti-inflammatory action of saikosaponin was not due to its directory action on abrenal cortex or hypothalamus.8. The hemolytic action of Shosaikoto was correlated with the affinity of saikosaponin to membrane, moreover, with acti-inflammatory action and glucocorticoid releaing action.9. Long term administration of Shosaikoto enhanced the responsiveness of liver to Glucocorticoid. Less

本研究以与汉方相似的生药组成的小柴胡和大柴胡为研究对象，探讨了汉方中生药组成和多元成分的意义，明确了以下事实。Shosaikoto可能通过减少巨噬细胞前列腺素的产生，刺激白细胞介素-1的释放和吞噬来调节免疫反应. Shosaikoto刺激IL-1的释放是由于减少前列腺素的产生和其他未知的机制.小柴胡汤和大柴胡汤对白细胞介素-1释放的作用相反，这分别是由于小柴胡汤和大柴胡汤所特有的生药的作用，以及它们所共有的生药的辅助作用. Shosaikoto能降低高脂血症家兔血中单核/巨噬细胞的数量，并能增加血清胆固醇。相反，大柴胡组则保持与对照组相同的水平.使用Sho的组件 ...更多信息 通过乙醇溶解度和分子大小的分级，研究了小柴胡汤对巨噬细胞吞噬功能的促进作用。分子量小于1000的乙醇不溶性组分和乙醇可溶性组分的吞噬刺激活性与Shosaikoto相当或更高.通过改变小柴胡药材组成，研究其成分含量的变化，结果表明，黄芪、大枣、柴胡和人参对小柴胡中甘草皂苷的提取有促进作用，而黄芪和半夏则使其减少，这种减少是由于甘草皂苷吸收到药材残渣中所致.柴胡皂苷的抗炎作用不是通过对肾上腺皮质或下丘脑的直接作用实现的. Shosaikoto的溶血作用与柴胡皂苷的膜亲和性有关，此外还与抗炎作用和糖皮质激素释放作用有关.长期服用小柴胡汤可增强肝脏对糖皮质激素的反应性。少

项目成果

Makoto Inoue: "Response of liver to Glucocorticoid is Altered by Administration of Shosaikoto (Kampo Medicine)" Chem.Pharm.Bull. 38. 418-421 (1990)

Makoto Inoue：“服用 Shosaikoto（汉方药）会改变肝脏对糖皮质激素的反应”Chem.Pharm.Bull。

Yuko Nagatsu: "Modification of Macrophage Functions by Shosaikoto(Kampo Medicine)Leads to Enhancement of Immune Response" Chem.Pharm.Bull.37. 1540-1542 (1989)

Yuko Nagatsu：“Shosaikoto（汉方医学）对巨噬细胞功能的修饰导致免疫反应增强”Chem.Pharm.Bull.37。