权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Basic and clinical evaluation of Juzen-taiho-to usedin atopic dermatitis

十善太凤汤治疗特应性皮炎的基础和临床评价

基本信息

批准号：
15590607
负责人：
OGIHARA Yukio
金额：
$ 2.37万
依托单位：
Meijo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Atopic dermatitis (AD) is an acute, subacute or chronic pruritus dermatitis with other findings, such as xerosis, excoriations and lichenification, often occurring in person with asthma, allergic rhinoconjunctivitis, contact urticaria or with a family of the same. Itching and dryness of the skin are the cardinal symptoms the morphology of the lesions is the same as in other eczemas. More than 80% of the patients with AD have elevated levels of serum IgE with specificity to various environmental allergens to which they also demonstrated immediate-type skin reactions.The mainstay of therapy for AD remains topical steroids. However, there is no single treatment that is effective to completely cure the symptoms of AD in all patients at the present. Recently the immunosuppressive agents are also available but still not enough. Therefore, a variety of therapies are used for the treatment, including Kampo medicines in Japan. Kampo medicines are clinically and widely used for allergic and infl … More ammatory diseases but unfortunately their pharmacological properties and mechanisms of immunopharmacological actions are still not fully understood.In our study, we investigated the effects of these Kampo medicines on the onset and symptoms of AD model, induced by repeated application of 2,4,6-trinitrochlorobenzene in NC/Jic mice. We found that Juzen-taiho-to (JTT) significantly suppressed the symptoms and progress of AD-like dermatitis. Topical application of TNCB on ears once a week for 7 weeks gradually caused chronic edema with atopic dermatitis-like symptoms such as hemorrhage, excoriation, erosion and scaling. Serum total IgE level was also increased and massive invasion of activated mononuclear cells in epidermis and massive infiltration of eosinophils and neutrophils in dermis were histologically observed. Oral administration of JTT significantly suppressed the ear edema and progression of atopic dermatitis-like symptoms. It also reduced the increase of serum total IgE level and the infiltration of mononuclear cells in epidermis and eosinophils in dermis. In cervical lymph nodes, T cell ratio and CD4/CD8 ratio were significantly reduced in the control group but JTT improved their reduction, suggesting that JTT suppressed the traffic of lymphocytes from the draining lymph nodes into inflammatory lesion. RT-PCR analysis revealed that JTT suppressed the increase of mRNA expressions of IL-4 and IFNγ in cervical lymph nodes and TNFα, IL-1β, IL-4, eotaxin and CCR3 in ears, supporting the histological and flow-cytometric analysis. Furthermore, adoptive transfer experiments suggested that regulatory property might be involved in the suppressive actions of JTT in this chronic dermatitis. In conclusion, JTT could be regarded as a useful remedy for atopic dermatitis. Less

特应性皮炎(AD)是一种急性、亚急性或慢性瘙痒性皮炎，伴有其他症状，如干燥、剥脱和苔藓形成，通常发生在哮喘、过敏性鼻结膜炎、接触性荨麻疹或与上述患者的家族中。皮肤瘙痒和干燥是主要症状，皮损的形态与其他湿疹相同。超过80%的AD患者血清IgE水平升高，对各种环境过敏原具有特异性，他们也表现出即刻型皮肤反应。AD的主要治疗方法仍然是局部类固醇。然而，目前还没有单一的治疗方法可以完全治愈所有患者的阿尔茨海默病症状。最近，免疫抑制剂也有了，但仍然不够。因此，治疗方法多种多样，包括日本的Kampo药物。坎布族药物在临床上被广泛用于治疗过敏性和非传染性…这些药物对反复应用2，4，6-三硝基氯苯所致的NC/JIC小鼠AD模型的发病和症状的影响进行了研究。我们发现菊岑-泰豪-托(JTT)能显著抑制AD样皮炎的症状和进展。耳部局部应用TNCB，每周一次，持续7周，逐渐引起慢性水肿，并伴有特应性皮炎样症状，如出血、刮除、糜烂和鳞屑。血清总IgE水平升高，组织学观察到表皮内活化的单个核细胞大量侵袭，真皮内有大量嗜酸性粒细胞和中性粒细胞的浸润。口服JTT可显著抑制耳肿胀和特应性皮炎样症状的进展。降低血清总IgE水平的升高，减少表皮单个核细胞和真皮嗜酸性粒细胞的浸润。颈淋巴组织中，对照组T细胞比例和CD4/CD8比值明显降低，而健颈通络汤改善了T细胞比例和CD4/CD8比例的降低，提示健颈通络口服液抑制了引流淋巴结淋巴细胞向炎性病变的转运。逆转录聚合酶链式反应结果显示，颈淋巴组织中IL-4、干扰素γ和耳部肿瘤坏死因子α、IL-1β、IL-4、嗜酸性粒细胞趋化因子和CCR3mRNA的表达均受到抑制，支持组织学和流式细胞术分析。此外，采用转移实验表明，JTT在慢性皮炎中的抑制作用可能与调节特性有关。综上所述，该药可作为治疗特应性皮炎的有效药物。较少