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Studies on plasma membrane proteins which are specifically induced by cold acclimation in plants.

植物冷驯化特异性诱导的质膜蛋白的研究。

基本信息

批准号：
01480009
负责人：
YOSHIDA Shizuo
金额：
$ 3.78万
依托单位：
(The Institute of Low Temparature Science) Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

The objective of this study was to identify plasma membrane proteins which are specifically induced by cold acclimation in wheats (Triticum aestivum L.). Two cultivars with a marked difference in the genetic capability to harden, namely spring wheat13EA\ : (cv Chinese Spring) and winter wheat (cv Norstar) were used as the experimental materials. After four weeks of growth in a cold chamber, the freezing tolerance in the shoots of winter wheat increased to -18ﾟC, whereas only to -8ﾟC in the shoots of spring wheat. In roots from both cultivars, the freezing tolerance increased very slightly after the growth under the cold environment. Cold hardening induced remarkable changes in electrophoresis patterns of plasma membrane proteins depending on c13EA\ : ultivars and tissues. Polypeptides with molecular masses ranged from 22 to 31 kDa decreased commonly in the root and shoot plasma membranes from both cultivars. of these polypeptides, 28 and 26 kDa polypeptides decreased abrupty after one week of th13EA\ : e cold hardening. On the other hand, polypeptides with molecular masses of 89, 83, 23, 18 and 17 kDa increased specifically in the shoots of winter hardy wheat. The increases of the 23, 18 and 17 kDa polypeptides were proportional to the development13EA\ : of freezing tolerance. A freeze-fracture electronmicroscopy of plasma membranes from shoot cells revealed that the intramembrane particle number on the fracture faces decreased significantly in winter wheat after cold acclimation, but less in sprin13EA\ : g wheat. These results may suggest that the plasma membrane undergoes a molecular reorganization during cold acclimation in such manner that they are stabilized against freeze-induced dehydration stress.

本研究的目的是鉴定小麦（Triticum aestivum L.）冷驯化特异诱导的质膜蛋白。本试验以两个抗硬性遗传差异显著的品种，即春小麦13 EA：（中国春）和冬小麦（北极）为材料。在低温箱中生长4周后，冬小麦芽的抗冻性提高到-18 ° C，而春小麦芽的抗冻性仅提高到-8 ° C。在低温环境下生长后，两个品种根系的抗冻性略有提高。冷锻炼诱导c13 EA品种和组织质膜蛋白电泳图谱发生显著变化。多肽分子量范围从22至31 kDa的下降普遍在两个品种的根和地上部质膜。其中28和26 kDa多肽在冷锻炼1周后降低了细胞活力。另一方面，分子量为89，83，23，18和17 kDa的多肽增加，特别是在冬季哈代小麦的芽。23、18和17 kDa多肽的增加与抗冻性的发展成正比。低温锻炼后，冬小麦茎细胞质膜的冷冻断裂电镜观察表明，断裂面上的膜内颗粒数明显减少，而sprin 13 EA：g小麦的减少较少。这些结果可能表明，质膜在冷驯化过程中经历了分子重组，以这种方式，他们对冷冻诱导的脱水胁迫稳定。