An existence of cold stable vacuolar H^+-ATPase in Plants and its structural properties.

植物冷稳定液泡H^-ATP酶的存在及其结构特性。

基本信息

项目摘要

The cold stability of vacuolar H^+-ATPase in vivo is markedly different between mung bean (Vigna radiata L.) and pea (Pisum sativum L.), the plants which are representatives of cold-sensitive and cold-tolerant species, respectively. Upon cold exposure of mung bean seedlings the H^+-ATPase activity declined significantly soon after the start of the cold treatment. In pea seedlings, by contrast, the H^+-ATPase remained highly active for long periods of the cold treatment. On cold incubation of isolated vacuolar membranes in vitro in the presence of MgATP and chaotropic anions such as C1^-, NO^-_2 and NO^-_3, the vacuolar H^+-ATPases exhibited also a marked difference in the cold-induced destabilization between these plants, especially in the sensitivity to the concentration of the anions. The enzyme from mung bean appeared to be more susceptible to the anions, particularly to NO^-_2, than the enzyme from pea, resulting in more severe cold-inactivation at the lower concentrations. The MgATP-dependent cold inactivation of the H^+-ATPase was closely associated with a release of the peripheral sectors from the membranes in the same way as in vacuolar-type H^+-ATPases from wide range of organisms. Thus, the cold stability of the enzyme complex both in vivo and in vitro is considered to be dependent on the intrinsic properties of the enzyme, suggesting the existence of "cold-labile" and "cold-stable" types in plants. SDS-PAGE and immunoblot analysis of the subunit components of the H^+-ATPase from both plants revealed that the molecular structures, especially the epitopes of the membrane sectors (16kD), differ each other, albeit that the molecular masses of other minor subunits are also slightly different. These findings in the present study are considered to provide a new insight into the mechanism to control the cold stability of vacuolar H^+-ATPase in plants.
绿豆(Vigna radiata L.)和豌豆(Pisum sativum L.),分别是冷敏感和耐寒植物的代表。绿豆幼苗经冷处理后,H^+-ATPase活性在冷处理开始后不久即显著下降。相比之下,豌豆幼苗的H^+-ATP酶在长时间的低温处理中仍保持高度活性。离体液泡膜在MgATP和离液阴离子(如Cl ^-、NO^-2和NO^-3)存在下冷孵育时,液泡H^+-ATP酶在冷诱导的不稳定性方面也表现出明显的差异,特别是对阴离子浓度的敏感性。绿豆酶比豌豆酶更易受阴离子的影响,尤其是对NO2-的影响,在较低浓度下,绿豆酶的冷失活更为严重。MgATP依赖的H^+-ATP酶冷失活与细胞膜周边部分的释放密切相关,其方式与许多生物体中的液泡型H^+-ATP酶相同。因此,在体内和体外的酶复合物的冷稳定性被认为是依赖于酶的内在性质,这表明在植物中存在的“冷不稳定”和“冷稳定”类型。对两种植物H^+-ATPase亚基组分的SDS-PAGE和免疫印迹分析表明,分子结构,特别是膜部分(16 kD)的表位,彼此不同,尽管其他次要亚基的分子量也略有不同。这些结果为植物液泡H^+-ATPase冷稳定性的调控机制提供了新的认识。

项目成果

期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hotsubo,K.and Yoshida,S.: "Characterization of vacuolar H^+-ATPase from plants sensitive and tolerant to cold with respect to the stability against MgATP-induced cold inactivation in vitro." (submitted to plant Physiol.1995).
Hotsubo,K. 和 Yoshida,S.:“对寒冷敏感和耐受的植物液泡 H2-ATP 酶在体外针对 MgATP 诱导的冷失活的稳定性进行表征。”
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T.Shimmen and S.Yoshida: "Analysis of temperature dependence of cytoplasmic streaming using tonoplast-free cells of Characeae" Protoplasma. 176. 174-177 (1993)
T.Shimmen 和 S.Yoshida:“使用 Characeae 的无液泡膜细胞分析细胞质流的温度依赖性”原生质体。
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Zhou,B-L.,Arakawa,K.,Fujikawa,S.and Yoshida,S.: "Cold-induced alterations in plasma membrane proteins that are specifically related to the development of freezing tolerance in cold-hardy winter wheat." Plant Cell Physiol.35. 175-182 (1994)
Zhou,B-L.、Arakawa,K.、Fujikawa,S.和 Yoshida,S.:“寒冷诱导的质膜蛋白变化与耐寒冬小麦的耐冻性发展特别相关。”
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Koike, M., Arakawa, K.and Yoshida, S.: "Accumuilations of 19-kD plasma membrane protein and late embryogenensis-related proteins in winter wheat (Triticum aestivum L.cv.Chihoku) during the induction of freezing by ABA." Plant Physiol.(to be submitted).
Koike, M.、Arakawa, K. 和 Yoshida, S.:“在 ABA 诱导冷冻过程中,冬小麦 (Triticum aestivum L.cv.Chihoku) 中 19-kD 质膜蛋白和晚期胚胎发生相关蛋白的积累。
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Yoshida,S.: "Low temperature-induced alkalization of vacuoles in suspension cultured cells of mung bean(Vigna radiata L.)" Plant Cell Physiol.36. 1075-1079 (1995)
Yoshida,S.:“绿豆(Vigna radiata L.)悬浮培养细胞中液泡的低温诱导碱化”植物细胞生理学.36。
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YOSHIDA Shizuo其他文献

YOSHIDA Shizuo的其他文献

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{{ truncateString('YOSHIDA Shizuo', 18)}}的其他基金

FLOW STRUCTURES DETERMINING RIVER LEVEL IN AN ESTUARY
决定河口河流水位的水流结构
  • 批准号:
    13450199
  • 财政年份:
    2001
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Entrainment Phenomena in a Stratified Flow with Average Velocities and Turbulent Surface Layers
具有平均速度和湍流表面层的分层流中的夹带现象
  • 批准号:
    09450186
  • 财政年份:
    1997
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
UNSTEADY FACTORS AFFECTING SALT DIFFUSION IN THE STRATIFIED FLOW SYSTEM AT A RIVER MOUTH
影响河口层流系统盐分扩散的不稳定因素
  • 批准号:
    04452231
  • 财政年份:
    1992
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Effects of cold-induced dysfunction of vacuoles upon homeostasis in intracellular ionic environment.
寒冷诱导的液泡功能障碍对细胞内离子环境稳态的影响。
  • 批准号:
    03454005
  • 财政年份:
    1991
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Studies on plasma membrane proteins which are specifically induced by cold acclimation in plants.
植物冷驯化特异性诱导的质膜蛋白的研究。
  • 批准号:
    01480009
  • 财政年份:
    1989
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Molecular mechanisms of chilling injury and freezing injury
冷害和冻害的分子机制
  • 批准号:
    62304004
  • 财政年份:
    1987
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Co-operative Research (A)
Time-Space Variations of Hydraulic Parameters and Mixing Processes at the Density interface of River Mouth Flows
河口流密度界面水力参数及混合过程的时空变化
  • 批准号:
    62460158
  • 财政年份:
    1987
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Studies on deformation mechanisms of regional metamorphic rocks by means of microfabric analyses
区域变质岩变形机制的微结构分析研究
  • 批准号:
    61420015
  • 财政年份:
    1986
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (A)
Development of a laser optical cryomicroscope for the study of freeze-preservation of plant gene resources.
开发用于植物基因资源冷冻保存研究的激光光学冷冻显微镜。
  • 批准号:
    60840020
  • 财政年份:
    1985
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research

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