An existence of cold stable vacuolar H^+-ATPase in Plants and its structural properties.

植物冷稳定液泡H^-ATP酶的存在及其结构特性。

基本信息

项目摘要

The cold stability of vacuolar H^+-ATPase in vivo is markedly different between mung bean (Vigna radiata L.) and pea (Pisum sativum L.), the plants which are representatives of cold-sensitive and cold-tolerant species, respectively. Upon cold exposure of mung bean seedlings the H^+-ATPase activity declined significantly soon after the start of the cold treatment. In pea seedlings, by contrast, the H^+-ATPase remained highly active for long periods of the cold treatment. On cold incubation of isolated vacuolar membranes in vitro in the presence of MgATP and chaotropic anions such as C1^-, NO^-_2 and NO^-_3, the vacuolar H^+-ATPases exhibited also a marked difference in the cold-induced destabilization between these plants, especially in the sensitivity to the concentration of the anions. The enzyme from mung bean appeared to be more susceptible to the anions, particularly to NO^-_2, than the enzyme from pea, resulting in more severe cold-inactivation at the lower concentrations. The MgATP-dependent cold inactivation of the H^+-ATPase was closely associated with a release of the peripheral sectors from the membranes in the same way as in vacuolar-type H^+-ATPases from wide range of organisms. Thus, the cold stability of the enzyme complex both in vivo and in vitro is considered to be dependent on the intrinsic properties of the enzyme, suggesting the existence of "cold-labile" and "cold-stable" types in plants. SDS-PAGE and immunoblot analysis of the subunit components of the H^+-ATPase from both plants revealed that the molecular structures, especially the epitopes of the membrane sectors (16kD), differ each other, albeit that the molecular masses of other minor subunits are also slightly different. These findings in the present study are considered to provide a new insight into the mechanism to control the cold stability of vacuolar H^+-ATPase in plants.
绿豆(Vigna radiata L.)和豌豆(Pisum sativum L.)分别是冷敏感植物和耐冷植物的代表,体内液泡H^+-ATP酶的冷稳定性存在显着差异。绿豆幼苗在冷暴露后,H^+-ATP酶活性在冷处理开始后不久就显着下降。相比之下,在豌豆幼苗中,H^+-ATP酶在长时间的冷处理中仍保持高度活性。在存在 MgATP 和离液阴离子(如 C1^-、NO^-_2 和 NO^-_3)的情况下体外冷孵育分离的液泡膜时,液泡 H^+-ATP 酶在这些植物之间的冷诱导不稳定方面也表现出显着差异,特别是对阴离子浓度的敏感性。来自绿豆的酶似乎比来自豌豆的酶更容易受到阴离子,特别是NO^-_2的影响,导致在较低浓度下更严重的冷失活。 H^+-ATP酶的MgATP依赖性冷失活与膜周围部分的释放密切相关,其方式与来自多种生物体的液泡型H^+-ATP酶相同。因此,酶复合物在体内和体外的冷稳定性被认为取决于酶的内在特性,表明植物中存在“冷不稳定”和“冷稳定”类型。对两种植物的 H^+-ATPase 亚基成分进行 SDS-PAGE 和免疫印迹分析表明,尽管其他次要亚基的分子量也略有不同,但分子结构,特别是膜部分(16kD)的表位彼此不同。本研究的这些发现被认为为控制植物液泡H^+-ATP酶的冷稳定性机制提供了新的见解。

项目成果

期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hotsubo,K.and Yoshida,S.: "Characterization of vacuolar H^+-ATPase from plants sensitive and tolerant to cold with respect to the stability against MgATP-induced cold inactivation in vitro." (submitted to plant Physiol.1995).
Hotsubo,K. 和 Yoshida,S.:“对寒冷敏感和耐受的植物液泡 H2-ATP 酶在体外针对 MgATP 诱导的冷失活的稳定性进行表征。”
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T.Shimmen and S.Yoshida: "Analysis of temperature dependence of cytoplasmic streaming using tonoplast-free cells of Characeae" Protoplasma. 176. 174-177 (1993)
T.Shimmen 和 S.Yoshida:“使用 Characeae 的无液泡膜细胞分析细胞质流的温度依赖性”原生质体。
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Zhou,B-L.,Arakawa,K.,Fujikawa,S.and Yoshida,S.: "Cold-induced alterations in plasma membrane proteins that are specifically related to the development of freezing tolerance in cold-hardy winter wheat." Plant Cell Physiol.35. 175-182 (1994)
Zhou,B-L.、Arakawa,K.、Fujikawa,S.和 Yoshida,S.:“寒冷诱导的质膜蛋白变化与耐寒冬小麦的耐冻性发展特别相关。”
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Koike, M., Arakawa, K.and Yoshida, S.: "Accumuilations of 19-kD plasma membrane protein and late embryogenensis-related proteins in winter wheat (Triticum aestivum L.cv.Chihoku) during the induction of freezing by ABA." Plant Physiol.(to be submitted).
Koike, M.、Arakawa, K. 和 Yoshida, S.:“在 ABA 诱导冷冻过程中,冬小麦 (Triticum aestivum L.cv.Chihoku) 中 19-kD 质膜蛋白和晚期胚胎发生相关蛋白的积累。
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Yoshida,S.: "Low temperature-induced alkalization of vacuoles in suspension cultured cells of mung bean(Vigna radiata L.)" Plant Cell Physiol.36. 1075-1079 (1995)
Yoshida,S.:“绿豆(Vigna radiata L.)悬浮培养细胞中液泡的低温诱导碱化”植物细胞生理学.36。
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YOSHIDA Shizuo其他文献

YOSHIDA Shizuo的其他文献

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{{ truncateString('YOSHIDA Shizuo', 18)}}的其他基金

FLOW STRUCTURES DETERMINING RIVER LEVEL IN AN ESTUARY
决定河口河流水位的水流结构
  • 批准号:
    13450199
  • 财政年份:
    2001
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Entrainment Phenomena in a Stratified Flow with Average Velocities and Turbulent Surface Layers
具有平均速度和湍流表面层的分层流中的夹带现象
  • 批准号:
    09450186
  • 财政年份:
    1997
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
UNSTEADY FACTORS AFFECTING SALT DIFFUSION IN THE STRATIFIED FLOW SYSTEM AT A RIVER MOUTH
影响河口层流系统盐分扩散的不稳定因素
  • 批准号:
    04452231
  • 财政年份:
    1992
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Effects of cold-induced dysfunction of vacuoles upon homeostasis in intracellular ionic environment.
寒冷诱导的液泡功能障碍对细胞内离子环境稳态的影响。
  • 批准号:
    03454005
  • 财政年份:
    1991
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Studies on plasma membrane proteins which are specifically induced by cold acclimation in plants.
植物冷驯化特异性诱导的质膜蛋白的研究。
  • 批准号:
    01480009
  • 财政年份:
    1989
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Molecular mechanisms of chilling injury and freezing injury
冷害和冻害的分子机制
  • 批准号:
    62304004
  • 财政年份:
    1987
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Co-operative Research (A)
Time-Space Variations of Hydraulic Parameters and Mixing Processes at the Density interface of River Mouth Flows
河口流密度界面水力参数及混合过程的时空变化
  • 批准号:
    62460158
  • 财政年份:
    1987
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Studies on deformation mechanisms of regional metamorphic rocks by means of microfabric analyses
区域变质岩变形机制的微结构分析研究
  • 批准号:
    61420015
  • 财政年份:
    1986
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (A)
Development of a laser optical cryomicroscope for the study of freeze-preservation of plant gene resources.
开发用于植物基因资源冷冻保存研究的激光光学冷冻显微镜。
  • 批准号:
    60840020
  • 财政年份:
    1985
  • 资助金额:
    $ 4.29万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research

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