Effects of cold-induced dysfunction of vacuoles upon homeostasis in intracellular ionic environment.
寒冷诱导的液泡功能障碍对细胞内离子环境稳态的影响。
基本信息
- 批准号:03454005
- 负责人:
- 金额:$ 4.16万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1991
- 资助国家:日本
- 起止时间:1991 至 1992
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Chilling sensitivity of mung bean cultured cells varied markedly depending on the growth phases. At the initial stages after subculture, cells were relatively insensitive to chilling, but chilling sensitivity increased abruptly thereafter and reached a maximum at the early stage of exponential growth. Upon further culturing, cell become chilling-tolerant, becoming most tolerant at the stage of stationary growth. We observed that cold sensitivity of the tonoplast H^+-ATPase in vivo changed markedly as a function of growth cycle. the enzyme was very sensitive to cold at the early stage of exponential growth and less sensitive at the late stage of exponential growth. Therefore, there exists an intimate correlation between sensitivities of cells to cold and the coldinsability of the H^+-ATPase. Biochemical studies on the cold-induced inactivation of the tonoplast H^+-ATPase indicated that a selective release of the peripheral subunits as a whole from the membrane sectors (16 kDa subunit).Changes in cytoplasmic pH in mung bean cultured cells were observed utilizing a cryomicroscope and a fluorescence ratio imaging system with special reference to the variations in chilling sensitivity during cell growth. In the extremely chilling-sensitive cells at the early stage of exponential growth, cytoplasmic pH was reduced abruptly upon cold incubation at 0゚C. However, in chilling- tolerant cells at the late stage of exponential growth, no change in the cytoplasmic pH was detected even after protracted chilling for up to 72 h.From the results, it is suggested that vacuolar proton translocating systems has an important role for maintaining of cell homeostasis, especially under such a cold environment.
绿豆培养细胞的冷冻敏感性根据生长阶段而显着变化。传代培养初期,细胞对低温相对不敏感,但此后低温敏感性急剧增加,并在指数生长期达到最大值。经过进一步培养,细胞变得耐冷,在稳定生长阶段变得最耐受。我们观察到体内液泡膜 H^+-ATP 酶的冷敏感性随着生长周期的变化而发生显着变化。该酶在指数生长初期对寒冷非常敏感,在指数生长后期较不敏感。因此,细胞对冷的敏感性与H^+-ATP酶的耐冷性之间存在着密切的相关性。对寒冷诱导的液泡膜 H^+-ATPase 失活的生化研究表明,外围亚基作为一个整体从膜部分(16 kDa 亚基)选择性释放。利用冷冻显微镜和荧光比成像系统观察绿豆培养细胞中细胞质 pH 的变化,特别是冷冻的变化 细胞生长过程中的敏感性。在指数生长早期对低温极度敏感的细胞中,0°C 冷培养后细胞质 pH 值突然降低。然而,在指数生长后期的耐冷细胞中,即使延长冷藏72小时也没有检测到细胞质pH值的变化。结果表明,液泡质子易位系统对于维持细胞稳态具有重要作用,特别是在如此寒冷的环境下。
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Yoshida,S.: "Chilling-induced inacfivation and its recovery of tonoplast H^+-ATPase in mung bean cell suspension cultures." Plant Physiology. 95. 456-460 (1991)
Yoshida,S.:“绿豆细胞悬浮培养物中液泡膜 H+-ATP 酶的低温诱导失活及其恢复。”
- DOI:
- 发表时间:
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- 影响因子:0
- 作者:
- 通讯作者:
Yoshia,S.,Hattanda,Y.,Suyana,T.: "Variations in the chilling sensitivity of suspension-cultured cells of mung bean(Vigna radiata[L.]Wilegeh)during the growth cycle." Plant and Cell Physiology.
Yoshia,S.、Hattanda,Y.、Suyana,T.:“绿豆(Vigna radiata[L.]Wilegeh)悬浮培养细胞在生长周期中的冷冻敏感性变化。”
- DOI:
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- 影响因子:0
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Matsuura-Endo,C.,Meshima,M.,Yoshida,S.: "Mechanism of the declime in Vacuolar H^+_-ATPase aetivity in mcng bean hypocotyls during chilling." Plant Physiology. 100. 718-722 (1992)
Matsuura-Endo,C.、Meshima,M.、Yoshida,S.:“冷却过程中 mcng 豆下胚轴液泡 H^_-ATP 酶活性下降的机制。”
- DOI:
- 发表时间:
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- 影响因子:0
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- 通讯作者:
Yoshida,S.,Matsuura-Endo,C.: "Comparison of temperature dependenay of tonoplast protm translocation between plants sensitive and insensitivo to chilling" Plant Physiology. 95. 504-508 (1991)
Yoshida,S.,Matsuura-Endo,C.:“对寒冷敏感和不敏感的植物之间液泡膜蛋白易位的温度依赖性的比较”植物生理学。
- DOI:
- 发表时间:
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- 影响因子:0
- 作者:
- 通讯作者:
Toshida,S.: "Chilling-induced inactivation and its recovery of tonoplast H^+_-ATPase in mung bean cell suspenspension cultures." Plant Physiology. 95. 456-460 (1991)
Toshida,S.:“绿豆细胞悬浮培养物中液泡膜 H^_-ATP 酶的低温诱导失活及其恢复。”
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- 影响因子:0
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YOSHIDA Shizuo其他文献
YOSHIDA Shizuo的其他文献
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13450199 - 财政年份:2001
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$ 4.16万 - 项目类别:
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Entrainment Phenomena in a Stratified Flow with Average Velocities and Turbulent Surface Layers
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09450186 - 财政年份:1997
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An existence of cold stable vacuolar H^+-ATPase in Plants and its structural properties.
植物冷稳定液泡H^-ATP酶的存在及其结构特性。
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05454012 - 财政年份:1993
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04452231 - 财政年份:1992
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Studies on plasma membrane proteins which are specifically induced by cold acclimation in plants.
植物冷驯化特异性诱导的质膜蛋白的研究。
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01480009 - 财政年份:1989
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Molecular mechanisms of chilling injury and freezing injury
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62304004 - 财政年份:1987
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Grant-in-Aid for Co-operative Research (A)
Time-Space Variations of Hydraulic Parameters and Mixing Processes at the Density interface of River Mouth Flows
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Studies on deformation mechanisms of regional metamorphic rocks by means of microfabric analyses
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61420015 - 财政年份:1986
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Development of a laser optical cryomicroscope for the study of freeze-preservation of plant gene resources.
开发用于植物基因资源冷冻保存研究的激光光学冷冻显微镜。
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60840020 - 财政年份:1985
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