Effects of cold-induced dysfunction of vacuoles upon homeostasis in intracellular ionic environment.
寒冷诱导的液泡功能障碍对细胞内离子环境稳态的影响。
基本信息
- 批准号:03454005
- 负责人:
- 金额:$ 4.16万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1991
- 资助国家:日本
- 起止时间:1991 至 1992
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Chilling sensitivity of mung bean cultured cells varied markedly depending on the growth phases. At the initial stages after subculture, cells were relatively insensitive to chilling, but chilling sensitivity increased abruptly thereafter and reached a maximum at the early stage of exponential growth. Upon further culturing, cell become chilling-tolerant, becoming most tolerant at the stage of stationary growth. We observed that cold sensitivity of the tonoplast H^+-ATPase in vivo changed markedly as a function of growth cycle. the enzyme was very sensitive to cold at the early stage of exponential growth and less sensitive at the late stage of exponential growth. Therefore, there exists an intimate correlation between sensitivities of cells to cold and the coldinsability of the H^+-ATPase. Biochemical studies on the cold-induced inactivation of the tonoplast H^+-ATPase indicated that a selective release of the peripheral subunits as a whole from the membrane sectors (16 kDa subunit).Changes in cytoplasmic pH in mung bean cultured cells were observed utilizing a cryomicroscope and a fluorescence ratio imaging system with special reference to the variations in chilling sensitivity during cell growth. In the extremely chilling-sensitive cells at the early stage of exponential growth, cytoplasmic pH was reduced abruptly upon cold incubation at 0゚C. However, in chilling- tolerant cells at the late stage of exponential growth, no change in the cytoplasmic pH was detected even after protracted chilling for up to 72 h.From the results, it is suggested that vacuolar proton translocating systems has an important role for maintaining of cell homeostasis, especially under such a cold environment.
绿豆培养细胞的冷敏感性随生长阶段的不同而有明显的差异。继代培养初期,细胞对低温相对不敏感,但此后低温敏感性急剧增加,并在指数生长初期达到最大值。在进一步培养时,细胞变得耐冷,在稳定生长阶段变得最耐冷。我们观察到,液泡膜H^+-ATPase的冷敏感性在体内作为生长周期的函数发生显著变化。该酶在指数生长初期对低温非常敏感,在指数生长后期对低温不敏感。因此,细胞对冷的敏感性与H^+-ATP酶的耐冷性之间存在着密切的关系。低温诱导液泡膜H^+-ATP酶失活的生化研究表明,液泡膜H^+-ATP酶的周边亚基(16 kDa亚基)整体选择性地从膜上释放出来。利用低温显微镜和荧光比率成像系统观察了绿豆培养细胞的细胞质pH变化,特别是细胞生长过程中对低温敏感性的变化。在极冷敏感细胞的指数生长的早期阶段,细胞质的pH值突然下降后,在0 ℃冷孵育。然而,在耐冷细胞的后期阶段的指数增长,没有变化的细胞质的pH值检测,即使在延长冷却长达72小时。从结果表明,液泡质子转运系统具有重要的作用,维持细胞内稳态,特别是在这样的寒冷环境。
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Yoshida,S.: "Chilling-induced inacfivation and its recovery of tonoplast H^+-ATPase in mung bean cell suspension cultures." Plant Physiology. 95. 456-460 (1991)
Yoshida,S.:“绿豆细胞悬浮培养物中液泡膜 H+-ATP 酶的低温诱导失活及其恢复。”
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- 影响因子:0
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- 通讯作者:
Yoshia,S.,Hattanda,Y.,Suyana,T.: "Variations in the chilling sensitivity of suspension-cultured cells of mung bean(Vigna radiata[L.]Wilegeh)during the growth cycle." Plant and Cell Physiology.
Yoshia,S.、Hattanda,Y.、Suyana,T.:“绿豆(Vigna radiata[L.]Wilegeh)悬浮培养细胞在生长周期中的冷冻敏感性变化。”
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Yoshida,S.,Matsuura-Endo,C.: "Comparison of temperature dependenay of tonoplast protm translocation between plants sensitive and insensitivo to chilling" Plant Physiology. 95. 504-508 (1991)
Yoshida,S.,Matsuura-Endo,C.:“对寒冷敏感和不敏感的植物之间液泡膜蛋白易位的温度依赖性的比较”植物生理学。
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- 影响因子:0
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Matsuura-Endo,C.,Meshima,M.,Yoshida,S.: "Mechanism of the declime in Vacuolar H^+_-ATPase aetivity in mcng bean hypocotyls during chilling." Plant Physiology. 100. 718-722 (1992)
Matsuura-Endo,C.、Meshima,M.、Yoshida,S.:“冷却过程中 mcng 豆下胚轴液泡 H^_-ATP 酶活性下降的机制。”
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- 影响因子:0
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- 通讯作者:
Yoshida,S.Hattanda,Y.Suyama,T.: "Variatons in the chilling sensitivity of suspension-cultured cells of mung bean(Vigna radiata〔L.〕Wilegek)during the growth cycle." Plant and Physiology.
Yoshida, S. Hattanda, Y. Suyama, T.:“绿豆(Vigna radiata [L.] Wilegek)悬浮培养细胞在生长周期中的冷冻敏感性变化。”
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- 影响因子:0
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YOSHIDA Shizuo其他文献
YOSHIDA Shizuo的其他文献
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13450199 - 财政年份:2001
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$ 4.16万 - 项目类别:
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An existence of cold stable vacuolar H^+-ATPase in Plants and its structural properties.
植物冷稳定液泡H^-ATP酶的存在及其结构特性。
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05454012 - 财政年份:1993
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04452231 - 财政年份:1992
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Studies on plasma membrane proteins which are specifically induced by cold acclimation in plants.
植物冷驯化特异性诱导的质膜蛋白的研究。
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01480009 - 财政年份:1989
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62304004 - 财政年份:1987
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Time-Space Variations of Hydraulic Parameters and Mixing Processes at the Density interface of River Mouth Flows
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61420015 - 财政年份:1986
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60840020 - 财政年份:1985
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