Study on the mechanism of regulation of PSI/PSII stoichiometry

PSI/PSII化学计量调控机制研究

• 批准号: 01480017
• 负责人: FUJITA Yoshihiko
• 金额: $ 4.29万
• 依托单位: Okazaki National Research Institutes, National Institute for Basic Biology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480017/
• 关键词: Chl a synthesis; Cyanophytes; Cytochrome b_6; PSI; PSII stoichiometry; Photosynthesis; PSI synthesis; Thylakoids; ラン藻; 形成調節; クロロフィルa合成; シトクロムb__ー_6-f__ー; クロロフィルa; チトクロムb_6ーf

Light-energy conversion in oxygenic photosynthesis is driven by cooperative actions of PSI and PSII. Thus, the efficiency of energy conversion depends strictly on the balance between the two photoreactions. We have found that the balance is maintained by regulation of PSI/PSII stoichiometry through the control of PSI synthesis induced by the state of thylakoid ETS. Our further study has focused on (i) the relationship between the state of ETS and regulation of PSI synthesis, (ii) the site controlled in the process of PSI synthesis. For the problems (1), examination of ETS state has been done by flah-induced oxidation-reduction of thylakoid components before and after acclimation. For (ii), Chl a synthesis and synthesis of psaA/B polypeptides have been examined under various photosynthetic conditions. Results have indicated that (1) HQNO-sensitive Cyt b_6 oxidation is monitored, (2) greater size of this reaction causes stimulation of PSI synthesis, (3) control of PSI synthesis occurs at least at the terminal step (s) of Chl a synthesis. Regulation of transcription of psaA/B seems not to be involved in this control. Based on these results, a work model for the mechanism of regulation of PSI formation, the signal, the signal transduction and the control site of PSI synthesis, has been proposed.

氧合光合作用中的光能转换是由PSI和PSII协同作用驱动的。因此，能量转换的效率严格取决于两个光反应之间的平衡。我们发现，通过控制类囊体Ets状态诱导的PSI合成来调节PSI/PSII的化学计量比来维持这种平衡。我们的进一步研究集中在(I)Ets状态与PSI合成调控之间的关系，(Ii)PSI合成过程中受控制的部位。对于问题(1)，通过驯化前后类囊体类囊体成分的氧化还原来检测ETS的状态。对于(II)，我们研究了在不同的光合作用条件下，叶绿素a的合成和psaA/B多肽的合成。结果表明：(1)HQNO敏感的Cytb_6氧化受到监控；(2)反应规模增大导致PSI合成受到刺激；(3)PSI合成受控至少发生在Chla合成的末期(S)。这种调控似乎与psaA/B的转录调控无关。在此基础上，提出了PSI形成的调控机制、PSI合成的信号、信号转导和控制部位的工作模型。

项目成果

池谷 透,大城 香,高橋 正征,藤田 善彦: "Photosynthetic pigment system of picophytoplankton of cyanophytes isolated from subsurface water in Kuroshio area" Journal of the Oceanographical Society of Japan. 48. (1992)

Toru Ikeya、Kaori Oshiro、Masaaki Takahashi、Yoshihiko Fujita：“从黑潮地区地下水中分离出的微型浮游植物的光合色素系统”，日本海洋学会杂志 48。（1992）。

藤田 善彦,岩間 由美,大城 香,村上 明男,萩原 直樹: "Regulation of lightーharvesting antennae in response to light intesity in the green alga Chlorella pyrenoidosa." Plant Cell Physiology. 30. 1029-1037 (1989)

Yoshihiko Fujita、Yumi Iwama、Kaori Oshiro、Akio Murakami、Naoki Hagiwara：“绿藻蛋白核小球藻光强度的调节”，植物细胞生理学 30。1029-1037。

大城 香,藤田 善彦: "Complementary chromatic adaptation in the cyanobacterium Tolypothrixtenuis:Location of phycoerythrin newly synthesizedby green illumination." Plant Cell Physiology. 32. 827-834 (1991)

Kaoru Oshiro、Yoshihiko Fujita：“蓝藻 Tolypothrixtenuis 中的互补色适应：绿色照明新合成的藻红蛋白的位置。植物细胞生理学”32. 827-834 (1991)。

大橋 和子,村上 明男,田中 歩,辻 英夫,藤田 善彦: "Developmental changes in amounts of thylakoid components in plastids of barley leaves." Plant Cell Physiology.

Kazuko Ohashi、Akio Murakami、Ayumu Tanaka、Hideo Tsuji、Yoshihiko Fujita：“大麦叶质体中类囊体成分数量的发育变化。”

村上 明男,藤田 善彦: "Regulation of photosystem stoichiometry in the photosynthetic system of the cyanophyte Synechocysis PCC 6714 in response lightーintensity." Plant Cell Physiology. 32. 223-230 (1991)

Akio Murakami、Yoshihiko Fujita：“响应光强度的蓝藻集胞藻 PCC 6714 光合系统中的光系统化学计量调节。”32. 223-230 (1991)。