ANTICANCER AGENTS FROM CYANOPHYTES AND MARINE ORGANISMS

这项研究的主要目标是发现新的天然产物 蓝藻(蓝藻)和有藻的海洋动物 共生菌将作为治疗癌症或其疾病的药物有用 预防和/或作为研究化学物质的药理学工具 致癌。Ne分离的蓝绿藻将在 用于抗癌评估的培养。到目前为止，研究集中在 主要是在生长迅速、相对丰富的蓝藻上发现 主要存在于陆地生态系统中，属于该目 诺斯托卡莱斯。拟议的研究将集中在增长较慢的和 更稀有的蓝绿藻，特别是在海洋环境中发现的那些。 海绵、被囊动物和其他海洋无脊椎动物 蓝藻和原叶绿藻也将被收集用于抗癌 评估。养殖蓝藻和海洋动物的提取物 将制备含藻类共生体并进行细胞毒性测试 使用KB细胞系(一种人表皮样癌 鼻咽部)。这些提取物也将被评估为促进肿瘤。 利用Epstein-Barr病毒诱导的抗肿瘤促进活性 12-0-十四酰佛波醇-13-乙酸酯转化Raji细胞的研究 化验。使用这些生物测定来监测活性物质的分级 原则在最有希望的线索中，细胞毒性，促进肿瘤， 和抗肿瘤促进化合物将被分离出来，它们的分子 结构，包括绝对的立体化学，将通过 化学方法和物理方法的结合。细胞毒剂 将在全国范围内进一步评估选择性细胞毒性 癌症研究所的人类癌细胞系小组。例如， 培养的伯氏青藻Scytonema burmanicum(UH)细胞毒素的研究 菌株D)-4-1)和Fortii Plectonema fortii(UH菌株DO-321-2)。 含有海绵沙鼠耳屏。关岛，以及那些含有藻类的 将从关岛分离、鉴定和鉴定被囊双毛虫SG-37 被评估为抗癌剂。

The main goal of this research is to discover new natural products from blue-green algae (cyanobacteria) and marine animals possessing algal symbionts that will be useful as drugs in the treatment of cancer or its prevention and/or as pharmacological tools for the study of chemical carcinogenesis. Ne isolates of blue-green algae will be grown in culture for anticancer evaluation. Research to-date has concentrated mainly on the rapid-growing, relatively abundant cyanophytes found predominantly in terrestrial ecosystems and belonging to the order Nostocales. The proposed research will focus on the slower-growing and rarer blue-green algae, particularly those found in marine environments. Sponges, tunicates, and other marine invertebrates that harbor cyanophytes and prochlorophytes will also be collected for anticancer evaluation. Extracts of the cultured cyanophytes and the marine animals containing algal symbionts will be prepared and tested for cytotoxicity using the KB cell line (a human epidermoid carcinoma of the nasopharynx). The extracts will also be evaluated for tumor-promoting and anti-tumor-promoting activity using the Epstein-Barr virus-induced transformation by 12-0-tetradecanoylphorbol-13-acetate in Raji cells assay. Using these bioassays to monitor fractionation of the active principles in the most promising leads, the cytotoxic, tumor-promoting, and anti-tumor-promoting compounds will be isolated and their molecular structures, including absolute stereochemistries, will be elucidated by a combination of chemical and physical methods. The cytotoxic agents will be evaluated further for selective cytotoxicity in the National Cancer Institute's human cancer cell line panel. For example, the potent cytotoxins in the cultured cyanophytes Scytonema burmanicum (UH isolate D)-4-1) and Plectonema fortii (UH isolate DO-321-2), the algal- containing sponge Sarcotragus sp. for Guam, and that algal-containing didemnid tunicate SG-37 from Guam will be isolated, identified, and evaluated as anticancer agents.