ANTICANCER AGENTS FROM CYANOPHYTES AND MARINE ORGANISMS

来自蓝藻和海洋生物的抗癌剂

• 批准号: 3163672
• 负责人: RICHARD E MOORE
• 金额: $ 21.31万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-08-01 至 1994-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3163672
• 关键词: Cnidaria; Cyanophyta; Epstein Barr virus; Invertebrata; Pacific Islands; Porifera; alternatives to animals in research; animal extract; antineoplastics; bioassay; biological products; chemical carcinogenesis; chemical structure; cytotoxicity; drug design /synthesis /production; drug metabolism; drug screening /evaluation; gel electrophoresis; microorganism culture; nasopharynx; nuclear magnetic resonance spectroscopy; oral pharyngeal neoplasm; physical separation; saltwater environment; stereochemistry; thin layer chromatography; tissue /cell culture

The main goal of this research is to discover new natural products from blue-green algae (cyanobacteria) and marine animals possessing algal symbionts that will be useful as drugs in the treatment of cancer or its prevention and/or as pharmacological tools for the study of chemical carcinogenesis. Ne isolates of blue-green algae will be grown in culture for anticancer evaluation. Research to-date has concentrated mainly on the rapid-growing, relatively abundant cyanophytes found predominantly in terrestrial ecosystems and belonging to the order Nostocales. The proposed research will focus on the slower-growing and rarer blue-green algae, particularly those found in marine environments. Sponges, tunicates, and other marine invertebrates that harbor cyanophytes and prochlorophytes will also be collected for anticancer evaluation. Extracts of the cultured cyanophytes and the marine animals containing algal symbionts will be prepared and tested for cytotoxicity using the KB cell line (a human epidermoid carcinoma of the nasopharynx). The extracts will also be evaluated for tumor-promoting and anti-tumor-promoting activity using the Epstein-Barr virus-induced transformation by 12-0-tetradecanoylphorbol-13-acetate in Raji cells assay. Using these bioassays to monitor fractionation of the active principles in the most promising leads, the cytotoxic, tumor-promoting, and anti-tumor-promoting compounds will be isolated and their molecular structures, including absolute stereochemistries, will be elucidated by a combination of chemical and physical methods. The cytotoxic agents will be evaluated further for selective cytotoxicity in the National Cancer Institute's human cancer cell line panel. For example, the potent cytotoxins in the cultured cyanophytes Scytonema burmanicum (UH isolate D)-4-1) and Plectonema fortii (UH isolate DO-321-2), the algal- containing sponge Sarcotragus sp. for Guam, and that algal-containing didemnid tunicate SG-37 from Guam will be isolated, identified, and evaluated as anticancer agents.

这项研究的主要目的是从 蓝绿色藻类（蓝细菌）和具有藻类的海洋动物 与癌症治疗的药物有用的共生体 预防和/或作为化学研究的药理工具 致癌作用。 蓝绿色藻类的NE分离株将在 用于抗癌评估的培养。 研究待定已经集中 主要是在快速增长的相对丰富的蓝植物上 主要在陆地生态系统中，属于秩序 怀旧。 拟议的研究将集中于增长较慢和 稀有的蓝绿色藻类，尤其是在海洋环境中发现的藻类。 海绵，双齿衣和其他海洋无脊椎动物 还将收集蓝植物和亲绿植物的抗癌 评估。 培养的蓝植物和海洋动物的提取物 将准备并测试含有藻类共生体的细胞毒性 使用KB细胞系（人类表皮样癌 鼻咽）。 提取物还将评估以进行肿瘤促进 使用爱泼斯坦 - 巴尔病毒诱导的抗肿瘤促进活性 拉吉细胞中12-0-四烷烯基-13-乙酸酯的转化 测定。 使用这些生物测定来监视活动的分数 最有前途的铅的原理，细胞毒性，肿瘤促进 和抗肿瘤促进化合物将被隔离，其分子 结构，包括绝对立体化的体化，将由 化学和物理方法的结合。 细胞毒性剂 将进一步评估国家的选择性细胞毒性 癌症研究所的人类癌细胞系列。 例如， 培养的蓝植物中有效的细胞毒素scytonema burmanicum（UH 分离株D）-4-1）和Plectonema fortii（UH分离DO-321-2），藻类 包含海绵sarcotragus sp。关岛和那个含藻类的 关岛的didemnid pundicate SG-37将被隔离，识别，并且 评估为抗癌剂。