Effects of Biologically Active Substances on Function and Morphology of Farm Animal Spermatozoa.

生物活性物质对家畜精子功能和形态的影响。

• 批准号: 01480088
• 负责人: MASAKI Junji
• 金额: $ 1.66万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480088/
• 关键词: Spermatozoa; Seminal plasma; Zona-free hamster egg; Phospholipase A_2; Maitotoxin; cAMP-dependent protein kinase; cAMP-independent protein kinase; Acrosome reaction; ホスホリパ-ゼA; cAMP; プロテインキナ-ゼ; 家蓄精液; 家蓄精子; 生理活性物質; ウシ精漿; ヤギ精子; 透明帯除去卵子; Caイオ

Effects of three biologically active substances on the function and morphology of mammalian spermatozoa were studied.Phospholipase A_2, of which activity has been detected in the seminal plasma of bull, goat and human, accelerated acrosome reaction of hamster spermatozoa. Addition of this enzyme to bull epididymal spermatozoa increased the rate of their entry into zona-free hamster egg. This effect was indefinite for bull ejaculated spermatozoa, suggesting that they had already received influence of enzyme from seminal plasma.Maitotoxin, a toxic and Ca^<2+>-dependent substance of marine origin, was used to examine whether it can induce the acrosome reaction as does Ca ionophore (A23187) in the mammalian spermatozoa. Maitotoxin inhibited motility and removed acrosome in the bull and goat spermatozoa when some 10 ng/ml were added. The less concentration (1-2ng/ml) induced almost normal acrodome reaction of spermatozoa and increased their entry into zona-free hamster egg. These results show that maitotoxin may be useful for inducer of acrosome reaction.Goat spermatozoa were used to extract cAMP-independent protein kinase and their substrate protein. Analyses of the purified material have shown that the protein kinase is similar to casein kinase II and there are peptides which are phosphorylated by this enzyme.

研究了三种生物活性物质对哺乳动物精子功能和形态的影响。在公牛、山羊和人的精浆中检测到磷脂酶A_2的活性，它加速仓鼠精子顶体反应。将这种酶添加到公牛附睾精子中增加了它们进入无透明带仓鼠卵子的速率。这种效应对于公牛射精的精子来说是不确定的，表明它们已经受到了精浆酶的影响。Maitotoxin是一种海洋来源的有毒且Ca^2+依赖性物质，被用来检查它是否可以像哺乳动物精子中的Ca离子载体(A23187)一样诱导顶体反应。添加约 10 ng/ml 的麦芽毒素会抑制公牛和山羊精子的运动并去除顶体。较低的浓度（1-2ng/ml）诱导了几乎正常的精子顶峰反应，并增加了它们进入无透明带仓鼠卵的机会。这些结果表明麦毒毒素可能是顶体反应的诱导剂。利用山羊精子提取cAMP非依赖性蛋白激酶及其底物蛋白。对纯化材料的分析表明，蛋白激酶与酪蛋白激酶 II 相似，并且存在被该酶磷酸化的肽。

项目成果

渡邊 伸也: "透明体除去ハムスタ-卵子内への精子浸入能に及ぼすホスホリパ-ゼA_2の効果" 家蓄繁殖誌.

Shinya Watanabe：“磷脂酶 A_2 对精子穿透透明仓鼠卵子能力的影响”《家庭育种杂志》。

浜野光市: "牛精子の先体反応に及ぼすマイトトキシンの影響" 家畜繁殖学雑誌. (1990)

Hikaru Hamano：“线粒体毒素对牛精子顶体反应的影响”《牲畜繁殖杂志》（1990 年）。

康 〓秀: "ヤギ精子の運動性、呼吸および先体の形態に及ぼすマイトトキシンの影響" 家畜繁殖誌.

康舒：“丝裂毒素对山羊精子运动、呼吸和顶体形态的影响”畜牧育种杂志。

HAMANO koichi: "Effect of maitotoxin on acrosome reaction of bull spermatozo." J. Anim. Reprod.

HAMANO koichi：“麦芽毒素对公牛精子顶体反应的影响。”

SHIMAZAKI Takuya: "Characterization of casein kinaseII-like protein kinase and its potential substrates in sperm of sea urchin." J. Biochemistry.

ShiMAZAKI Takuy​​a：“海胆精子中酪蛋白激酶 II 样蛋白激酶及其潜在底物的表征。”