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Studies on the biologically active substances in the semen of domestic animals

家畜精液中生物活性物质的研究

基本信息

批准号：
59480076
负责人：
MASAKI Junji
金额：
$ 4.1万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1984
资助国家：
日本
起止时间：
1984 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59480076/
关键词：
Phospholipase <A_2>Inhibin Hormone 生理活性物質精液

项目摘要

Mammalian semen contains biologically active substances or factors.Their dynamic status and significance in reproductive physiology, however, are not yet well understood. This study focussed to the two sorts of compounds, phospholipase A and inhibin which were known to occur in the seminal plasma of some farm animals. Our earlier observation has presented an evidence that the bull seminal plasma can induce a morphological change in the cell membrane. Since this activity was assumed to involve phospholipase A action, a comparative estimation of the enzyme activity was made with bull, boar and human seminal plasma. When used phosphatidylcholine as substrate, the phospholipase A activity in bull seminal plasma was the highest among the materials from the three species. The effect of bull seminal plasma on the sperm membrane was investigated with the methods of cytochemistry and immuno-electron microscopy, including Concanavalin A and cationized ferritin bindings, lactate and malate dehydr … More ogenase activities, and sperm motility. We found that the bull seminal plasma had a similar activity in part to that of a medium for in vitro fetilization on the epididymal spermatozoa. The results suggest that the bull seminal plasma may have a capacitation-accelerating factor. Futher study should be necce-ssary for the conclusion, including contribution of the enzyme.The inhibin activity of seminal plasma was estimated, mainly with the material from goat to investigate some biological and physico-chemical properties. We confirmed that in the goat at least, seminal inhibin originated exclusively from testis and the physico-chemical properties were not principally different between them. It showed a distinct seasonal fluctuation in a close relation to blood FSH levels.The enzyme activity of testicular fluid decreased during the passage of epididymis and in the process of ejaculation. Molecular weight of the testicular extracts was estimated to be about 14,000. Administration of the bull testicular extracts clearly decreased the blood FSH levels in the GnRH-treated immature steers. Less

哺乳动物精液中含有生物活性物质或因子。然而，它们在生殖生理学中的动态状态和意义尚不清楚。这项研究重点关注已知存在于一些农场动物精浆中的两种化合物：磷脂酶 A 和抑制素。我们早期的观察提供了证据表明公牛精浆可以诱导细胞膜的形态变化。由于假定该活性涉及磷脂酶 A 作用，因此用公牛、公猪和人精浆对酶活性进行了比较评估。以磷脂酰胆碱为底物时，公牛精浆中磷脂酶A活性是三个物种材料中最高的。采用细胞化学和免疫电镜方法研究了公牛精浆对精子膜的影响，包括刀豆蛋白A和阳离子铁蛋白结合、乳酸和苹果酸脱水酶活性以及精子活力。我们发现公牛精浆对附睾精子具有部分与体外受精培养基相似的活性。结果表明，公牛精浆可能具有获能加速因子。结论还需要进一步的研究，包括酶的贡献。对精浆的抑制素活性进行了测定，主要以山羊为材料，考察其一些生物学和理化性质。我们证实，至少在山羊中，精液抑制素完全源自睾丸，并且它们之间的理化性质没有主要差异。表现出明显的季节性波动，与血液FSH水平密切相关。睾丸液酶活性在附睾通过和射精过程中降低。睾丸提取物的分子量估计约为 14,000。施用公牛睾丸提取物明显降低了经 GnRH 处理的未成熟公牛的血液 FSH 水平。较少的