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The Role of Prostaglandins in Learning and Memory

前列腺素在学习和记忆中的作用

基本信息

批准号：
01480151
负责人：
WATANABE Yasuyoshi
金额：
$ 4.42万
依托单位：
Osaka Bioscience Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

1. Localization of prostaglandin endoperoxide synthase in the hippocampusImmunohistochemical localization of Prostaglandin Endoperoxide Synthase (PES) in monkey hippocampus was investigated by the use of monoclonal antibody against purified PES. PES-immunoreactivity was intense in the neurons in the hippocampu and cerebral cortex. Especially, the granular and pyramidal neurons in the dentate gyrus and Ammon's horn of the hippocampus showed the most intense immunoreactivity. In the pyramidal cells in CA3 region, positive staining was seen the apical dendrites as well as the proximal dendrites and somata.2. Effects of PGs on long-term potentiation in the CA3 neurons of guinea pig hippocamp sliceThe experimental set-up for long-term potentiation was established by using hippocampal slices of guinea pig. Prostaglandin (PG) s D2, E2, and F2alpha upto 20 muM ha no effects on excitatory post-synaptic potential and long-term potentiation of CA3 neurons by tetanic stimuli of dentate gyrus.3. Novel cytosolic phospholipase A2 in the rat brain and its translocationWhen phospholipase A2 (PLA2) activities were investigated in the rat brain, some activities with the major two peaks on the gel filtration column were found in the cytosolic fraction of the brain. Low molecular-weight form (PLAL) was active at neutral pH and absolutely required Ca^<2+> for its activity. In the reconstitution syste of partially purified PLAL and synaptosomal membranes from rat brain, PLAL associated with the membranes in a Ca^<2+>-dependent manner.4. Effect of PGE2 on the glutamate releaseBy using microdialysis technique, we investigated the effect of PGE2, whose receptor enriched in the hippocampus, on the release of glutamate which is considered to be major trigger for long-term potentiation in the hippocampus. PGE2 enhanced glutamate release in several portions of the brain.

1.前列腺素内过氧化物合酶（PES）在猴海马的定位应用抗PES单克隆抗体对猴海马内PES进行了免疫组织化学定位。PES免疫反应在海马和大脑皮质的神经元中是强烈的。其中以海马齿状回和阿蒙角的颗粒神经元和锥体神经元的免疫反应最强。在CA3区锥体细胞中，阳性染色见于顶树突、近端树突和胞体.前列腺素对豚鼠海马脑片CA3区神经元长时程增强的影响建立了豚鼠海马脑片长时程增强的实验装置。前列腺素D2、E2和F2 α在20 μ M以下对强直刺激齿状回引起的CA3区神经元兴奋性突触后电位和长时程增强无影响.一种新的大鼠脑胞浆磷脂酶A_2（PLA_2）及其转位研究了大鼠脑内的磷脂酶A_2（PLA_2）活性，发现在大鼠脑胞浆中有一些活性，在凝胶过滤柱上有两个主要的峰。低分子量形式（PLAL）在中性pH下具有活性，并且其活性绝对需要Ca^2+。在部分纯化的PLAL与大鼠脑突触体膜的重组系统中，PLAL以Ca^<2+>依赖的方式与突触体膜结合.前列腺素E_2对谷氨酸释放的影响本实验采用微透析技术，研究了在海马中富集的前列腺素E_2对谷氨酸释放的影响，而谷氨酸是海马长时程增强的主要触发因素。PGE2增强了大脑几个部分的谷氨酸释放。