Monoclonal Antibodies as a Diagnostic Tool for Helminth Infection

单克隆抗体作为蠕虫感染的诊断工具

• 批准号: 01480170
• 负责人: SENDO Fujiro
• 金额: $ 4.48万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480170/
• 关键词: monoclonal antibody; Schistosoma japonicum; Gnathostoma spinigerum; Trichinella spiralis; Trypanosoma cruzi; specific monoclonal antibody; Polyspicific monoclonal antibody; heterophile monoclonal antibody; 單クロ-ン抗体; 施毛虫; IgG1; IgM; Western blot

The fundamental tool for diagnosis of parasite infection is egg detection by fecal examination. However, in some parasite infection, it is impossible to detect eggs in the faces because of the specific life history of the worm. Furthermore, in endemic areas, it is sometimes difficult to find specialists skilled in microscopic diagnosis. For these reasons, immuno-sero diagnosis may be a useful approach, but as is well known, crude parasite antigens always show some degree of cross-reactivity with heterologous parasite antigens. To overcome this problem, purification of these antigens is critical. Affinity chromatography using a monoclonal antibody (MoAb) to a specific parasite appears a promising aid to diagnosis. Furthermore, production of recombinant protein using monoclonal antibody as a probe may be useful not only for diagnosis but for the development of a vaccine against parasite infection. With these points in mind, we attempted to establish MoAbs to various helminths as the first step to purification of diagnostic antigens.

诊断寄生虫感染的基本工具是通过粪便检查检测虫卵。然而，在一些寄生虫感染中，由于蠕虫的特定生活史，不可能在面部检测到虫卵。此外，在流行病地区，有时很难找到精通显微镜诊断的专家。由于这些原因，免疫血清学诊断可能是一种有用的方法，但众所周知，粗寄生虫抗原总是显示出一定程度的交叉反应性与异源寄生虫抗原。为了克服这个问题，这些抗原的纯化是至关重要的。亲和层析使用单克隆抗体（单克隆抗体）的特定寄生虫出现一个有前途的援助诊断。此外，使用单克隆抗体作为探针生产重组蛋白不仅可用于诊断，而且可用于开发抗寄生虫感染的疫苗。考虑到这些点，我们试图建立抗各种蠕虫的单克隆抗体，作为纯化诊断抗原的第一步。

项目成果

Pen Gao, Susumu Saito, Takao Yamashita, Tadashi Watanabe, and Fujiro Sendo: "Characterization of a Monoclonal Antibody that specifically recognizes a target antigen in Schistosoma japonicum adult worms" American Journal of Tropical Medicine & Hygiene.

Pen Gau、Susumu Saito、Takao Yamashita、Tadashi Watanabe 和 Fujiro Sendo：“特异性识别日本血吸虫成虫目标抗原的单克隆抗体的特性”美国热带医学杂志

Wichit Rojekittikhun,Susumu Saito,Takao Yamashita,Tadashi Watanabe,Prasert Setasuban.and Fujiro Sendo: "Three Monoclonal Antibodies against Advanced Thirdstage Larvae of Gnathostoma spinigerum" Japanese Journal of Parasitology. 40. 287-292 (1991)

Wichit Rojekittikhun、Susumu Saito、Takao Yamashita、Tadashi Watanabe、Prasert Setasuban. 和 Fujiro Sendo：“针对棘口吸虫晚期第三期幼虫的三种单克隆抗体”日本寄生虫学杂志。

Susumu Saito,Takao Yamashita,Pen Gao,Wichit Rojekittikhun and Fujiro Sendo: "Autoimmunity of hybridoma produced from spleen cells of BALB/c mice infected or immunized with parasites." Parasitological Immunology.

Susumu Saito、Takao Yamashita、Pen Gau、Wichit Rojekittikhun 和 Fujiro Sendo：“由感染或免疫寄生虫的 BALB/c 小鼠的脾细胞产生的杂交瘤的自身免疫。”

Wichit Rojekittikhun,Susumu Saito,Takao Yamashita,Tadashi Watanabe,Prasert Setasuban.and Fujiro Sendo: "Three Monoclonal Antibodies against Advenced Thirdーstage Larvae of <Gnathostoma>___ー <spinigerum>___ー" Japanese Journal of Parasitology.

Wichit Rojekittikhun、Susumu Saito、Takao Yamashita、Tadashi Watanabe、Prasert Setasuban. 和 Fujiro Sendo：“针对 <Gnathostoma>___— <spinigerum>___— 高级第三阶段幼虫的三种单克隆抗体”日本寄生虫学杂志。

Susumu Saito,Takao yamashita,Pen Gao,Wichit Rojekittikhum and Fujiro Sendo: "Production and properties of the immunized with the muscle larval antigen of <Trichinella>___ー <spiralis>___ー." Parasitological Immunology.

Susumu Saito、Takao yamashita、Pen Gau、Wichit Rojekittikhum 和 Fujiro Sendo：“<旋毛虫>_____ <螺旋虫>_____ 肌肉幼虫抗原免疫的生产和特性。”