Studies on origin and coevolution of Schistosoma japonicum in Asia

亚洲日本血吸虫起源与协同进化研究

• 批准号: 13576008
• 负责人: AGATSUMA Takeshi
• 金额: $ 7.1万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13576008/
• 关键词: Schistosomatidae; Schistosoma; Orientobilharzia; avian schistosome; mammalian schistosome; molecular phylogenetic tree; origin of Schistosoma; Coevolution; アジア; 日本住血吸虫; 起源; 進化; ミトコンドリアDNA; リボソームRNA遺伝子; 住血吸虫類; 28S; ITS2; CO1; 塩基配列; COI

To date, in order to examine the origin of the genus Schistosoma, phylogenetic relationships among the genus Schistosoma have been estimated using partial nucleotide sequences of CO1, 1srDNA and ITS2. In this study, whole nucleotide sequences of CO1, 1srDNA aqnd ssrDNA were determined for the first time, and on the basis of the sequence information, phylogenetic relationships were inferred among species from the family Schistosomatidae. Materials used in this study are in total 29 species in 10 genera consisting of 6 genera from the subfamily Schistosomatinae, two genera from the subfamily Bilharziellinae and two genera from the subfamily Gigantobilharziinae. The genus Chimaerohemecus in the family Sanguinicolidae was used as an outgroup. The number of nucleotides determined for CO1, ssrDNR and 1srDNA was 1122 bp and 3765 bp, respectively. In this study, combined nuleotide sequences including all of the three genes were used for tree analysis. It was estimated that the mammalianschistosomes were paraphyletic, since the clad of (Ornithobilharzia+Austrobilharzia) was supported to be a sister clad of (Schistosoma+Orientobilharzia). It was also found that Orientobilharzia was placed within the genus Schistosoma, and a sister of clad consisting of the African group and the S.indicum group. This result suggests that Orientobilharzia could be an ancestral species for them.There are two theories on the origin of Schistosoma. One is the African origin theory by Davis (1980,1992), proposing that Indian continental plate, which separated from the Gondwana Land, carried an ancestral species of Schistosoma from Africa to Asia. Another is the Asian origin theory (Snyder & Loker,2000; Agatsma et al., 2003; Locker et al., 2004) proposing that an ancestral species of Schistosoma occurred in Asia and expanded Asia to Africa through migration of a mammalian host. The present study supported the Asian origin theory again.

到目前为止，为了研究血吸虫属的起源，已经利用CO1、1srDNA和ITS2的部分核苷酸序列估计了血吸虫属之间的系统发育关系。本研究首次测定了CO1、1srDNA和SSrDNA的全核苷酸序列，并根据序列信息推测了血吸虫科各物种之间的系统发育关系。本研究共收集血吸虫亚科6属29种10属29种，其中血吸虫亚科Bilharziellinae 2属，巨人亚科Giantobilharziinae 2属。血吸虫科中的嵌合体属被用作外类群。测得CO1、ssrDNR和1srDNA的核苷酸长度分别为1122bp和3765bp。在本研究中，包括所有这三个基因的组合核苷酸序列被用于树分析。推测哺乳动物血吸虫是副血吸虫，因为(鸟毕吸虫+奥氏毕吸虫)的包膜被支持为(血吸虫+东方毕吸虫)的姊妹包膜。还发现东方毕氏吸虫被归入血吸虫属，并且是由非洲组和印度血吸虫组组成的外壳的姐妹。这一结果表明，东方毕吸虫可能是它们的祖先物种。关于血吸虫的起源有两种说法。一种是Davis(1980,1992)的非洲起源说，认为从冈瓦那大陆分离出来的印度大陆板块将血吸虫的祖先物种从非洲带到了亚洲。另一种是亚洲起源理论(Snyder&Loker，2000；Agatsma等人，2003；Locker等人，2004)，提出血吸虫的祖先物种发生在亚洲，并通过哺乳动物宿主的迁移将亚洲扩展到非洲。本研究再次支持了亚洲起源说。

项目成果

Takeshi Agatsuma: "Origin and evolution of Schistosoma japonicum (Review)"Parasitology International. 52. 335-340 (2003)

Takeshi Agatsuma：“日本血吸虫的起源和进化（评论）”国际寄生虫学。

Phylogenetic status of a lung fluke in the philippines based on mitochondrial genome.

基于线粒体基因组的菲律宾肺吸虫的系统发育状况。

• 发表时间: 2003
• 期刊: Japanese Journal of Tropical Medicine and Hygiene 31
• 作者: Yukita Sato;Moritoshi Iwagami;Jun Iwashita;Tatsuya Abe;Masayoshi Yukawa;Bayani L.Blas;Kenjiro Kawashima;Takeshi Agatsuma
• 通讯作者: Takeshi Agatsuma

Molecular taxonomic position of the elephant schistosome,Bivitellobilharzia nairi,newly discovered in Sri Lanka.

斯里兰卡新发现的大象血吸虫 Bivitellobilharzia nairi 的分子分类地位。

• 发表时间: 2004
• 期刊: Parasitology International 53
• 作者: Takeshi Agatsuma;R.P.V.J.Rajapakse;V.Y.Kuruwita;Moritoshi Iwagami;R.C.Rajapakse
• 通讯作者: R.C.Rajapakse

A.E.Lockyer, P.D.Olson, P.Ostergaard, D.Rollinson, D.A.Johnston, S.W.Attwood, V.R.Southgate, P.Horak, S.D.Snyder, T.H.Le, T.Agatsuma, et al.: "The phylogeny of the Schistosomatidae based on three genes with emphasis on the interrelationships of Schistosom

A.E.Lockyer、P.D.Olson、P.Ostergaard、D.Rollinson、D.A.Johnston、S.W.Attwood、V.R.Southgate、P.Horak、S.D.Snyder、T.H.Le、T.Agatsuma 等人：“血吸虫科的系统发育基于三个

T.H.Le, P-F.Humair, D.Blair, T.Agatsuma, D.T.J.Littlewood, D.P.McManus: "Mitochondrial gene content, arrangement and composition compared in Africa and Asian schistosomes"Molecular & Biochemical Parasitology. 117. 61-71 (2001)

T.H.Le、P-F.Humair、D.Blair、T.Agatsuma、D.T.J.Littlewood、D.P.McManus：“非洲和亚洲血吸虫线粒体基因含量、排列和组成的比较”分子