Studies on Iuduction of Autologous MLR in the Autoimmune Diseases and its Pathological Roles.

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On the specific nature and its biological significance of autologous mixed lymphocyte reaction (MLR), the following results are obtained. (1) Treatment with neuraminidase of the mixtures of stimulator cells and responder lymphocytes results in a marked enhancement of DNA replication in the autologous MLR. (2) Using this method, we are able to demonstrate that the autologous MLR is a helper T (TH) activation induced by DR antigens on accessory (AC) cells, of which the DNA replication is IL-2-independent, but dependent upon the multiple cytokines, IL-3, IL-5, IL-6 and IL-7. (3) The autologous MLR is also found to induce the generation of activated CD4^+ helper T cells, and also CD4^-CD8^- double negative (DN) T cells with a phenotype of alphabetaT cell receptor (alphabetaTcR)^+ or gammadeltaTcR^+. (4) Based on the findings of the specific nature of autologous MLR, the lymphoproliferation. Induced in the lymphoid tissues of MRL/lpr mouse is analyzed. As a result, it is found that abnormal proliferation of CD3^+CD4^-CD8^-B220^+ alphabetaTcR^+T cells in the lymphoid tissues may be a phenomenon in which precursors for this population can be proliferated in response to autologous accessory cells, mediated through certain antigens on the surface.