HLA-DR Antigen Positive Cells in the RA Synovium. -Their Significant Role Concerning in the Continuation of Chronic Inflammation of RA.

RA 滑膜中的 HLA-DR 抗原阳性细胞。

• 批准号: 63570138
• 负责人: MURAKAMI Kazuhiro
• 金额: $ 1.41万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570138/
• 关键词: Rheumatoid arthritis; Synovial cell; HLA-DR antigen; 清膜細胞; リウマトイド結節; HLAーDR陽性細胞; HLA; DR抗原; 螢光抗体法

1 Characteristics of RA synovial cells The sublining D cells which were remarkably proliferating in the rheumatoid synovial membrane were divided into two type cells, D(F) cells and D(M) cells immunohistochemically. In culture, D(F) cells showed high proliferative activity and easily transformed their morphological and functional character under the effects of variable cytokines. On the other hand, D(M) cells proliferated very slowly, and they contiguously and strongly expressed HLA-DR antigen on their surface. These findings suggest that D(F) cell plays an important role in formation of histopathological variety of RA synovitis and D(M) cell is largely concerned in the immunological activity of RA. 2 Histopathological study of early stage of synovial lesion We investigated the early stage of RA synovitis immunohistochemically and from this study it become clear that in very early of RA, HLA-DR positive cells were found in pericapillary region and following them T lymphocytes appeared and B lymphocytes appeared to aggregate in later. 3 The effects of drugs on RA synovitis Some patients with early stage RA showed improvement of condition of disease after treatment with immunomodulating drug (CCA), but no apparent qualitative and quantitative changes of HLA-DR positive cells in synovium were observed. These findings suggest the complexity of cellular interaction in development of RA synovitis. 4 Comparison of synovial cells with cells in rheumatoid nodule The characteristics of majority of cells in rheumatoid nodule were quite similar to that of D cells in rheumatoid synovitis. Clinically rheumatoid synovitis is characterized by its progressive and destructive nature in contrast to rheumatoid nodule. Histopathologically in the rheumatoid nodule the lymphocyte infiltration was less conspicuous. These findings suggest that lymphocyte infiltration is essential for the continuation of chronic destructive inflammation of RA synovitis.

1类风湿性关节炎滑膜细胞的特征在类风湿性关节炎滑膜细胞中增殖明显的亚层D细胞经免疫组化染色可分为D（F）细胞和D（M）细胞。在培养中，D（F）细胞表现出高增殖活性，并在各种细胞因子的作用下容易改变其形态和功能特性。另一方面，D（M）细胞增殖非常缓慢，并且它们在其表面上连续且强烈地表达HLA-DR抗原。提示D（F）细胞在RA滑膜炎组织病理变化中起重要作用，D（M）细胞与RA的免疫活性密切相关。2早期滑膜病变的组织学研究我们对RA早期滑膜炎进行了免疫组化观察，发现RA早期在毛细血管周围可见HLA-DR阳性细胞，随后出现T淋巴细胞，后期出现B淋巴细胞聚集。3药物对RA滑膜炎的影响部分早期RA患者经免疫调节剂（CCA）治疗后病情有所好转，但滑膜中HLA-DR阳性细胞的数量和质量无明显变化。这些发现表明RA滑膜炎发展中细胞相互作用的复杂性。4滑膜细胞与类风湿结节内细胞的比较类风湿结节内大部分细胞的特征与类风湿性滑膜炎中D细胞的特征十分相似。临床上类风湿性滑膜炎的特点是其进行性和破坏性的性质相反，类风湿性结节。组织学上类风湿结节淋巴细胞浸润不明显。这些结果表明，淋巴细胞浸润是必不可少的持续慢性破坏性炎症的RA滑膜炎。

项目成果

沢井 高志: "慢性関節リウマチ滑膜初期病変の免疫組識学的検討 ーモノクロナル抗体を用いた炎症性細胞の定性ならびに定量的解折ー" リウマチ. 30. 247-254 (1990)

Takashi Sawai：“类风湿性关节炎早期滑膜病变的免疫组织学研究 - 使用单克隆抗体对炎症细胞进行定性和定量分析”Rheumatology 30. 247-254 (1990)。

Sawai T, Murakami K, et al.: "Stromelysin synthesizing cells in the synovial tissue of rheumatoid arthritis demonstrated by in situ hybridization and immunohistochemical methods" Tohoku J. Exp. Med. 160. 285-286 (1990)

Sawai T、Murakami K 等人：“通过原位杂交和免疫组织化学方法证明类风湿性关节炎滑膜组织中溶质素合成细胞”Tohoku J. Exp。

沢井 高志: "慢性関節リウマチ最近の話題ーMetalloproteinaseとマトリックスの観点からー" 病理と臨床. 8. 1478-1484 (1990)

Takashi Sawai：“类风湿性关节炎的当前主题 - 从金属蛋白酶和基质的角度”病理学和临床研究 8. 1478-1484 (1990)。

村上 一宏: "リウマトイド結節構成細胞の解折 ー滑膜細胞との比較ー" 炎症. 10. 394-354 (1990)

Kazuhiro Murakami：“类风湿结节组成细胞的解剖 - 与滑膜细胞的比较”炎症。 10. 394-354 (1990)

村上 一宏: "ATLA抗体陽性の関節炎の2症例 ー免疫組織学的、電顕的観察ー" 最新医学. 45. 194-195 (1990)

Kazuhiro Murakami：“ATLA抗体阳性关节炎两例——免疫组织学和电子显微镜观察”现代医学45。194-195（1990）。