Public Health Meaning of Oxidative Stress in Aged Process

衰老过程中氧化应激的公共卫生意义

• 批准号: 01480205
• 负责人: SUMINO Kimiaki
• 金额: $ 3.58万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480205/
• 关键词: Oxidative Stress; Aging; Adult Disease; SOD; Fatty Acid; 泡沫・粥硬性; 生体膜; コレステロ-ル酸化生成物; 老化疾患(動脈硬化高血圧); SOD活性; TBA反応物

The components of lipids in focal tissues of patients suffered from adult diseases like arteriosclerosis, hypertension, diabetes, lung cancer and rheumatoid arthritis that were thought to be exposed oxidative stress, were checked using a GC/MS/COM.1. The oxidative form of saturated fatty acid were detected as monohydroxy form, but fatty acid in side chain of cholesterol were not changed.2. The oxidative form of unsaturated fatty acid were detected as to be peroxide and epoxide.3. Free and esteral cholesterol were metabolized to be oxidative form of cholesta-3, 5, 7-triene, cholesta-3, 5-diene-7, 8-epoxide, and 7-dehydrocholestero1-5alpha, 6alpha (or 5beta, 6beta) after trimethylsilization of N, O- (trimethylsilyl) -trifluoroactamide,as derived from dehydrocholesterol.4. Moreover, cholesterol- 5alpha, 6alpha (or 5beta, 6beta) -epoxide, cholesterol-6-hydroxide and cholesterol-6-methoxide were newly produced by cholesterol oxidation.5. After incubation of human monocytes with the cholestero1-5alpha, 6alpha-epoxide, monocytes were transformed to foam cell and large cell.6. Relative activity of superoside dismutase in young and old erythrocyte from diabetic patients and health people were measured, as to be resulted the same level in young erythrocytes, but significant difference in old erythrocytes.

使用GC/MS/COM检查患有成人疾病（如动脉硬化、高血压、糖尿病、肺癌和类风湿性关节炎，这些疾病被认为暴露于氧化应激）的患者病灶组织中的脂质成分。1.饱和脂肪酸的氧化形式主要为单羟基形式，而胆固醇侧链的脂肪酸没有发生变化.不饱和脂肪酸的氧化形式主要为过氧化物和环氧化合物.游离胆固醇和酯基胆固醇经N，O-（三甲基甲硅烷基）-三氟甲酰胺三甲基化后代谢为胆甾-3，5，7-三烯、胆甾-3，5-二烯-7，8-环氧化物和7-脱氢胆固醇1 - 5 α，6 α（或5 β，6 β）的氧化形式。此外，胆固醇氧化还新产生了胆固醇-5 α，6 α（或5 β，6 β）-环氧化物、胆固醇-6-氢氧化物和胆固醇-6-甲醇.将人单核细胞与胆固醇1 - 5 α，6 α-环氧化物孵育后，单核细胞转化为泡沫细胞和大细胞。本文测定了糖尿病患者和健康人青年和老年红细胞超氧化物歧化酶（SOD）的相对活性，结果表明，青年红细胞SOD相对活性相同，老年红细胞SOD相对活性有显著性差异。

项目成果

西村 容子: "酸化LDLのGC/MSによる脂質構成の分析(II)" 動脈硬化. 18. 492-492 (1990)

Yoko Nishimura：“通过 GC/MS 分析氧化 LDL 的脂质成分 (II)” 动脉硬化 18. 492-492 (1990)。

Nao Shibanuma: "Experimental Model of Pigmented Villondular Synovitis" The Journal of the Japanese Orthopaedic Association. 65. (1991)

Nao Shibanuma：“色素沉着绒毛滑膜炎的实验模型”日本骨科协会杂志。

Takaya Mio: "alpha-Hydroxy Fatty Acids of Cancerous Lung Tissues" Proceedings of Japanese Society for Biomedical Mass Spectrometry. 13. 201-206 (1988)

Takaya Mio：“癌性肺组织的α-羟基脂肪酸”日本生物医学质谱学会会刊。

Ryyoji Yamamoto: "SOD Activity and Unsaturated Fatty Acid in Erythrocyte" Japanese Journal of Hygiene. 46. 490 (1991)

Ryyoji Yamamoto：“红细胞中的 SOD 活性和不饱和脂肪酸”日本卫生杂志。

三尾 隆禰: "高血圧症の赤血球膜構成脂質の変化" 日本医用マスクペクトル学会講演集. 14. 189-192 (1989)

Takane Mio：“高血压中红细胞膜脂质的变化”日本医学面膜光谱协会会议记录 14. 189-192 (1989)。