Public Health Meaning of Oxidative Stress in Aged Process

使用GC/MS/COM.1对患有动脉硬化、高血压、糖尿病、肺癌和类风湿关节炎等成人疾病的患者局部组织中的脂质成分进行了检测，这些疾病被认为是暴露在氧化应激之下。结果表明：1.饱和脂肪酸的氧化形式为单羟基，而胆固醇侧链上的脂肪酸没有发生变化。检测到不饱和脂肪酸的氧化形式为过氧化和环氧化。由脱氢胆固醇衍生的N，O-(三甲基硅基)-三氟乙酰胺经三甲基硅化后，游离和酯基胆固醇被代谢成氧化形式的胆甾烷-3，5，7-三烯、胆甾烷-3，5-二烯-7，8-环氧化物和7-脱氢胆固醇1-5α，6α(或5β，6β)。此外，胆固醇氧化新生成了胆固醇-5α、6α(或5β、6β)-环氧化物、胆固醇-6-羟基和胆固醇-6-甲氧基。人单核细胞与胆固醇1-5α、6α-环氧化物孵育后，可转化为泡沫细胞和大细胞。对糖尿病患者和健康人的青年和老年红细胞中超氧化物歧化酶的相对活性进行了测定，结果表明，青年红细胞与健康人红细胞的超氧化物歧化酶相对活性相同，而老年红细胞的相对活性有显著差异。

The components of lipids in focal tissues of patients suffered from adult diseases like arteriosclerosis, hypertension, diabetes, lung cancer and rheumatoid arthritis that were thought to be exposed oxidative stress, were checked using a GC/MS/COM.1. The oxidative form of saturated fatty acid were detected as monohydroxy form, but fatty acid in side chain of cholesterol were not changed.2. The oxidative form of unsaturated fatty acid were detected as to be peroxide and epoxide.3. Free and esteral cholesterol were metabolized to be oxidative form of cholesta-3, 5, 7-triene, cholesta-3, 5-diene-7, 8-epoxide, and 7-dehydrocholestero1-5alpha, 6alpha (or 5beta, 6beta) after trimethylsilization of N, O- (trimethylsilyl) -trifluoroactamide,as derived from dehydrocholesterol.4. Moreover, cholesterol- 5alpha, 6alpha (or 5beta, 6beta) -epoxide, cholesterol-6-hydroxide and cholesterol-6-methoxide were newly produced by cholesterol oxidation.5. After incubation of human monocytes with the cholestero1-5alpha, 6alpha-epoxide, monocytes were transformed to foam cell and large cell.6. Relative activity of superoside dismutase in young and old erythrocyte from diabetic patients and health people were measured, as to be resulted the same level in young erythrocytes, but significant difference in old erythrocytes.