アルツハイマー病アミロイド・ベーター蛋白の毒性発現の抑制機構(メタロチオネイン及びその類似蛋白とアルミニウムの影響)
抑制阿尔茨海默病β淀粉样蛋白毒性的机制(金属硫蛋白及其类似蛋白和铝的影响)
基本信息
- 批准号:10470095
- 负责人:
- 金额:$ 3.9万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1998
- 资助国家:日本
- 起止时间:1998 至 1999
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Senile plaques are a major pathological hallmark of Alzheimer's disease (AD). They contain β-amyloid protein (βA), derived from amyloid precursor protein (APP). βA is thought to contribute to the pathogenesis of the disease, but the mechanism of its neurotoxicity remains unknown. Accumulated aluminum and thrombin have been expected to predispose βA to a toxic cause in neurons. Recently, an intracellular protein that binds βA and mediates neurotoxicity in AD, ERAB (endoplasmic-reticulum-associated binding protein), has been identified. To find a method to induce ERAB in neuronal cells. In the first fiscal year (1998), to determine whether aluminum induces ERAB mRNA, we grew neuroblastoma cells (GOTO) in serum-free RPMI 1640 medium containing aluminum sulfate (500μM) for 12 hours. The cells exposed to aluminum sulfate showed significantly lower APP mRNA level than the control cells. However, the cells exposed to aluminum sulfate showed no significant change in ERAB mRNA level, indicating that aluminum does not induce ERAB mRNA. In the second fiscal year (1999), to determine whether thrombin receptor activation induces ERAB mRNA, we grew neuroblastoma cells (GOTO) in serum-free RPMI 1640 medium containing TRAP (thrombin receptor activating peptide) (100μM) for 12 hours. The cells exposed to TRAP showed significantly higher APP mRNA level than the control cells. However, the cells exposed to TRAP showed no significant change in ERAB mRNA level, indicating that thrombin receptor activation does not induce ERAB mRNA. In conclusion, neither aluminum nor thrombin induces ERAB mRNA, suggesting that they do not relate to the pathogenesis of AD via ERAB mRNA induction.
老年斑是阿尔茨海默病(AD)的主要病理标志。它们含有源自淀粉样前体蛋白 (APP) 的 β-淀粉样蛋白 (βA)。 βA被认为与该疾病的发病机制有关,但其神经毒性的机制仍不清楚。累积的铝和凝血酶预计会使 βA 对神经元产生毒性作用。最近,已经鉴定出一种与 βA 结合并介导 AD 神经毒性的细胞内蛋白 ERAB(内质网相关结合蛋白)。寻找诱导神经细胞ERAB的方法。在第一个财政年度(1998),为了确定铝是否诱导 ERAB mRNA,我们在含有硫酸铝(500μM)的无血清 RPMI 1640 培养基中培养神经母细胞瘤细胞(GOTO)12 小时。暴露于硫酸铝的细胞显示APP mRNA水平显着低于对照细胞。然而,暴露于硫酸铝的细胞中ERAB mRNA水平没有显着变化,表明铝不会诱导ERAB mRNA。在第二财年(1999),为了确定凝血酶受体激活是否诱导ERAB mRNA,我们在含有TRAP(凝血酶受体激活肽)(100μM)的无血清RPMI 1640培养基中培养神经母细胞瘤细胞(GOTO)12小时。暴露于 TRAP 的细胞显示出比对照细胞显着更高的 APP mRNA 水平。然而,暴露于TRAP的细胞ERAB mRNA水平没有显着变化,表明凝血酶受体激活不会诱导ERAB mRNA。总之,铝和凝血酶均不诱导 ERAB mRNA,表明它们不通过 ERAB mRNA 诱导与 AD 的发病机制相关。
项目成果
期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hisahide Nishino et al.: "Hybrid survival motor neuron genes in Japanese patients with spinal muscular atrophy"Acta Neurological Scandinavica. 99 (6). 374-380 (1999)
Hisahide Nishino 等人:“日本脊髓性肌萎缩症患者的混合存活运动神经元基因”Acta Neuroological Scandinavica。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Hisahide Nishio et al: "Hybrid survial motor neuron genes in Japanese patients with spinal muscular atrophy"Acta Neurologica Scandinavica. 99(6). 374-380 (1999)
Hisahide Nishio 等人:“日本脊髓性肌萎缩症患者的混合存活运动神经元基因”Acta Neurologica Scandinavica。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Hisahide Nishino et al.: "High incidence of a survival motor neuron gene/cBCD541 gene ratio of 2 in Japanese Parents of spinal muscular atrophy patients : a characteristic background of spinal muscular atrophy in Japan?"J. Neuro. 246 (1). 48-52 (1999)
Hisahide Nishino 等人:“日本脊髓性肌萎缩症患者父母中运动神经元存活基因/cBCD541 基因比率为 2 的高发生率:日本脊髓性肌萎缩症的特征背景?”J.
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
"Japanese patient with spinal muscular atrophy"Acta Neurologica Scandinavica. 99(6). 374-380 (1999)
“日本脊髓性肌萎缩症患者”Acta Neurologica Scandinavica。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Hisahide Nishio et al: "High incidence of a survival motor neuron gene/^cBCD541 gene ratio of 2 in Japanese Parents of spinal muscular atrophy patients: a characteristic background of spinal muscular atrophy in Japan?"J Neuro. 246(1). 48-52 (1999)
Hisahide Nishio 等人:“日本脊髓性肌萎缩症患者父母中运动神经元存活基因/^cBCD541 基因比率为 2 的高发生率:日本脊髓性肌萎缩症的特征背景?”J Neuro。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
SUMINO Kimiaki其他文献
SUMINO Kimiaki的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('SUMINO Kimiaki', 18)}}的其他基金
Multi-Media Communication and Data Base in Disasters
灾难中的多媒体通信和数据库
- 批准号:
08557032 - 财政年份:1996
- 资助金额:
$ 3.9万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular Biological Analysis of Cells and Tissues from Animals Exposed to Chemicals and Ultraviolet Rays
对暴露于化学品和紫外线的动物的细胞和组织进行分子生物学分析
- 批准号:
08457115 - 财政年份:1996
- 资助金额:
$ 3.9万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Practical Application of Public Health Information Systems
公共卫生信息系统的实际应用
- 批准号:
01870030 - 财政年份:1989
- 资助金额:
$ 3.9万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B).
Public Health Meaning of Oxidative Stress in Aged Process
衰老过程中氧化应激的公共卫生意义
- 批准号:
01480205 - 财政年份:1989
- 资助金额:
$ 3.9万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Effects of Thiosulfydryl Substances on the Metabolism of Heavy Metals
硫代硫基物质对重金属代谢的影响
- 批准号:
60480190 - 财政年份:1985
- 资助金额:
$ 3.9万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
相似海外基金
Sustainable Technological Approach to Recycling Aluminum Manufacturing Waste Streams
回收铝制造废物流的可持续技术方法
- 批准号:
2902454 - 财政年份:2024
- 资助金额:
$ 3.9万 - 项目类别:
Studentship
CAS-Climate: Understanding the fundamental redox chemistry and transport of chloroaluminate anions in ionic liquid electrolytes to develop earth-abundant aluminum ion battery
CAS-Climate:了解离子液体电解质中氯铝酸盐阴离子的基本氧化还原化学和传输,以开发地球上丰富的铝离子电池
- 批准号:
2427215 - 财政年份:2024
- 资助金额:
$ 3.9万 - 项目类别:
Standard Grant
CAREER: Ultrawide Bandgap Aluminum Nitride FETs for Power Electronics
职业:用于电力电子器件的超宽带隙氮化铝 FET
- 批准号:
2338604 - 财政年份:2024
- 资助金额:
$ 3.9万 - 项目类别:
Continuing Grant
Infrared photonics using ferroelectric scandium-aluminum nitride semiconductors
使用铁电钪铝氮化物半导体的红外光子学
- 批准号:
2414283 - 财政年份:2024
- 资助金额:
$ 3.9万 - 项目类别:
Continuing Grant
Clarification of the aluminum alloy deposition mechanism under the electromagnetic circumstance in alternate current pulsed gas metal arc process applied wire arc additive manufacturing (WAAM)
阐明交流脉冲气体金属电弧工艺应用电弧增材制造(WAAM)电磁环境下铝合金沉积机理
- 批准号:
24K17530 - 财政年份:2024
- 资助金额:
$ 3.9万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Development of aluminum stabilized HTS coils for next-generation magnets with high radiation resistance and high magnetic field
开发用于下一代高抗辐射和高磁场磁体的铝稳定高温超导线圈
- 批准号:
23H03665 - 财政年份:2023
- 资助金额:
$ 3.9万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
GOALI: Data-driven design of recycling tolerant aluminum alloys incorporating future material flows
目标:数据驱动的可回收铝合金设计,结合未来的材料流
- 批准号:
2243914 - 财政年份:2023
- 资助金额:
$ 3.9万 - 项目类别:
Standard Grant
Optimization of a Self-Adjuvanting Particle System for Delivering Respiratory Syncytial Virus Prefusion Protein
用于输送呼吸道合胞病毒预融合蛋白的自我辅助颗粒系统的优化
- 批准号:
10666079 - 财政年份:2023
- 资助金额:
$ 3.9万 - 项目类别:
A Novel Sublingual Vaccine to Prevent Neisseria Gonorrhoeae Infection
预防淋病奈瑟菌感染的新型舌下疫苗
- 批准号:
10699065 - 财政年份:2023
- 资助金额:
$ 3.9万 - 项目类别:
AUGMENTING THE QUALITY AND DURATION OF THE IMMUNE RESPONSE WITH A NOVEL TLR2 AGONIST-ALUMINUM COMBINATION ADJUVANT
使用新型 TLR2 激动剂-铝组合佐剂增强免疫反应的质量和持续时间
- 批准号:
10933287 - 财政年份:2023
- 资助金额:
$ 3.9万 - 项目类别: