Effect of Campylobacter Pylori upon Immunological Conditions of Chronic Gastritis.

幽门弯曲杆菌对慢性胃炎免疫状况的影响。

• 批准号: 01480227
• 负责人: YACHI Akira
• 金额: $ 2.62万
• 依托单位: Sapporo Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480227/
• 关键词: Campylobacter pylori; Helicobacter pylori; Chronic Gastritis; Monoclinal antibody; Local immune response; Campylobacter pylori; 胃潰瘍; 十二指腸潰瘍; 組織学的重症度

Campylobacter pylori or Helicobacter pylori has recently been implicated as an etiologic agent of gastroduodenal disorders. Comparing the antibody to Helicobacter pylori in the sera of patients to that of normal controls by Western blot, a unique antibody was detected in the sera of patients, which reacted with the 25 Kilo molecular weight antigen of Helicobacter pylori. On the other hand, monoclinal antibody CP3 which was prepared in our laboratory also recognized the 25 Kilo molecular weight antigen of Helicobacter pylori. We then examined whether the serum antibody of the patient recognized the CP3 antigen purified by monoclinal antibody CP3. Western blot analysis demonstrated that the patient's serum strongly reacted with the affinity-purified CP3 antigen. By using monoclinal antibody CP3, We have established an enzyme-linked immunosorbent assay to detect CP3 antibody in sera. In chronic gastritis and gastric ulcer patients, the titer of CP3 antibody was significantly higher than that of normal controls and correlated with the histological grade of antral gastritis. The detection of CP3 antibody in sera is useful to diagnose chronic gastritis and gastric ulcer associated with Helicobacter pylori infection and also to evaluate the grade of gastritis.

幽门弯曲杆菌或幽门螺杆菌最近被认为是胃十二指肠疾病的病原体。用免疫印迹法对患者血清中抗幽门螺杆菌抗体与正常人血清进行比较，发现患者血清中存在一种特异性抗体，该抗体与幽门螺杆菌25Kilo抗原反应。本实验室制备的单抗CP 3也能识别幽门螺杆菌的25Kilo抗原。然后，我们检查患者的血清抗体是否识别由单斜抗体CP3纯化的CP3抗原。Western印迹分析表明，患者的血清与亲和纯化的CP3抗原强烈反应。利用单克隆抗体CP 3，建立了检测血清中CP 3抗体的酶联免疫吸附试验。慢性胃炎和胃溃疡患者血清CP 3抗体滴度明显高于正常对照组，且与胃窦炎的组织学分级有关。血清CP 3抗体的检测有助于慢性胃炎和幽门螺杆菌相关性胃溃疡的诊断及胃炎的分级。

项目成果

Sugiyama,T.: "A novel enzyme immunoassay for serodiagnosis of Helicobactor pylori infection." Gastroenterology. (1991)

Sugiyama,T.：“一种用于幽门螺杆菌感染血清诊断的新型酶免疫测定法。”

谷内昭: "Campylobacter pyloriと胃・十二指腸疾患ー免疫学的立場からー" 最新医学. 44. 258-262 (1989)

Akira Taniuchi：“幽门弯曲菌与胃和十二指肠疾病 - 从免疫学的角度来看”现代医学 44. 258-262 (1989)。

Imai, K.: "Detection of anti-Helicobacter pylori antibody of secretary IgA type in gastric jiuce." Gastroenterology. (1991)

Imai, K.：“胃液中分泌型 IgA 型抗幽门螺杆菌抗体的检测。”

杉山敏郎: "抗Campylobacter pyloriモノクロ-ナル抗体の作製と臨床応用" ライフサイエンス出版. 4. 1488-1494 (1989)

Toshiro Sugiyama：“抗幽门弯曲杆菌单克隆抗体的制备和临床应用”生命科学出版4. 1488-1494（1989）。

Yokota, K.: "Colonization of Helicobacter pylori in gastric mucosa of mongolian gerbils." Microbiology and Immunology. (1991)

Yokota, K.：“幽门螺杆菌在蒙古沙鼠胃粘膜中的定植。”