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Analysis of the auto-antibody in the serum from patients by use of anti-idiotypic monoclonal antibodies.

使用抗独特型单克隆抗体分析患者血清中的自身抗体。

基本信息

批准号：
62480191
负责人：
YACHI Akira
金额：
$ 2.56万
依托单位：
Sapporo Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480191/
关键词：
anti-idiotypic antibody Monoclonal antibody autoimmune disease

项目摘要

Model experiments using anti HLA monoclonal antibodies were performed to apply the immune response of anti-idiotypic antibodies to immunotherapy since anti-idiotypic antibodies are of interest in the investigation of a new immunotherapy since anti-idiotypic antibodies are of interest in the investigation of a new immunomodulator for cancer patients.Polyclonal anti-idiotypic antisera and anti-idiotypic monoclonal antibodies induced with anti HLA-A2. A28 monoclonal antibody KS1.The antigen rercognized by mouse monoclonal antibody YH206 (MoAb YH206) is mainly expressed in adenocarcinomas and also detected in the sera of cancer patients. Polyclonal and syngeneic monoclonal anti-idiotype antibodies were produced by immunization of MoAb YH206 (IGM) to a rabbit and a BALB/c mouse, respectively. Enzyme immunoassay showed that the anti-idiotype antibodies reacted specifically with MoAb YH206, but had no cross-reactivity with other monoclonal antibodies. Furthermore, the antibodies strongly inhibited the YH206 antigen-antibody reaction.By using these anti-idiotype antibodies, we screened sera of normal individuals and cancer patients with or without YH206 antigen for the detection of anti-YH206 antibody. Our study revealed that anti-YH206 antibody was detected in the sera of cancer patients, especially stomach and pancreas cancer patients but not in normal individuals. The results suggest that there may be an immune response against adenocarcinoma-associated antigen YH206 in cancer patients.

由于抗独特型抗体在研究癌症患者的新免疫调节剂中具有重要意义，因此使用抗HLA单克隆抗体进行模型实验以将抗独特型抗体的免疫应答应用于免疫治疗。小鼠单克隆抗体YH 206（MoAb YH 206）识别的抗原主要在腺癌中表达，也可在肿瘤患者血清中检测到。用单克隆抗体YH 206（IGM）免疫家兔和BALB/c小鼠，分别获得多克隆和同基因单克隆抗独特型抗体。酶免疫分析显示，抗独特型抗体与单克隆抗体YH 206特异性反应，与其它单克隆抗体无交叉反应。用这些抗独特型抗体对正常人和肿瘤患者血清进行抗YH 206抗体的检测，并与正常人和肿瘤患者血清进行比较。本研究发现，抗YH 206抗体在癌症患者血清中检测到，特别是胃癌和胰腺癌患者，而在正常人血清中未检测到。结果表明，在癌症患者中可能存在针对腺癌相关抗原YH 206的免疫应答。