Association of H. pylori with immunological aspects of chronic gastritis

幽门螺杆菌与慢性胃炎免疫学方面的关联

• 批准号: 03454229
• 负责人: YACHI Akira
• 金额: $ 3.39万
• 依托单位: Sapporo Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454229/
• 关键词: H. pylori; Chronic gastritis; Analysis of the Disease; Gastric juice; sIgA; ヘリコバクタ・ピロリ菌; モノクロ-ナル抗体; SIgA抗体

Helicobacter pylori has recently been implicated as an etiologic agent of gastroduodenal disorders. Comparing the antibody to H. pylori in the sera of patients with that of normal controls by Western blot analysis, a unique antiboy was detected in the sera of patients, which reacted with the 25-kilodalton antigen of H. pylori. On the other hand, monoclonal antibody CP3 prepared in the authors laboratory also recognized the 25-kilodalton antigen of H. pylori. Whether the serum antibody of the patient recognized the CP3 antigen purified by monoclonal antibody CP3 was then examined. Western blot analysis showed that the patient's serum reacted strongly with the affinity-purified CP3 antigen. Using monoclonal antibody CP3, an enzyme-linked immunosorbent assay to detect CP3 antibody in sera was established. In patients with chronic gastritis and gastric ulcers, the titer of CP3 antibody was significantly higher than in normal controls and correlated with the histological grade of antral gastritis. The detection of CP3 antibody in sera is useful in the diagnosis of chronic gastritis and gastric ulcer associated with H. pylori infection and also in evaluation of the grade of gastritis.

幽门螺杆菌最近被认为是胃十二指肠疾病的病原体。将抗体与H.用Western blot分析患者血清与正常人血清中幽门螺杆菌抗原的差异，结果在患者血清中检测到一种特异性抗幽门螺杆菌抗体，该抗体与幽门螺杆菌25kD抗原反应。幽门螺杆菌。另一方面，本实验室制备的单克隆抗体CP 3也能识别H.幽门。然后检查患者的血清抗体是否识别由单克隆抗体CP3纯化的CP3抗原。Western印迹分析表明，患者的血清与亲和纯化的CP3抗原反应强烈。利用单克隆抗体CP 3建立了检测血清中CP 3抗体的酶联免疫吸附试验。慢性胃炎和胃溃疡患者血清CP 3抗体滴度明显高于正常对照组，且与胃窦炎的组织学分级有关。血清CP 3抗体检测对慢性胃炎和H. pylori感染以及胃炎分级的评估。

项目成果

杉山 敏郎,他: "Helicobacter pylori感染胃粘膜のリンパ球抗体産生" Progress in Medicine. 12. 615-619 (1992)

Toshiro Sugiyama 等人：“感染幽门螺杆菌的胃粘膜中淋巴细胞抗体的产生”，医学进展 12. 615-619 (1992)。

千葉 満郎,他: "モノクローナル抗体CP2によるHelocobacter pyloriの免疫組織学的検討" 日本臨床免疫学会会誌. 15. 548-552 (1992)

Mitsuro Chiba 等人：“使用单克隆抗体 CP2 进行幽门螺杆菌的免疫组织学研究”日本临床免疫学会杂志 15. 548-552 (1992)。

Yachi,A. et al.: "Helicobacter pylori-associated gastroduodenal disease:Immunological aspects" Gastrointestinal Function,Regulation and Disturbances. 9. 131-139 (1991)

谷内，A.

Yachi,A.et al.: "Helicobacter pylori-associated gastroduodenal disease: Immunological aspects" Gastro in testinal Function,Regulation and Disturbances. 9. 131-139 (1991)

Yachi,A.et al.：“幽门螺杆菌相关胃十二指肠疾病：免疫学方面”胃肠道功能、调节和紊乱。

Yachi, A. et al: "Helicobacter pylori-associated gastroduodenal disease: Immunological aspects" Gastrointestinal Function, Regulation and Disturbances. 9. 131-139 (1991)

Yachi, A. 等人：“幽门螺杆菌相关胃十二指肠疾病：免疫学方面”胃肠功能、调节和紊乱。