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Energy metabolism of the severely infected

严重感染者的能量代谢

基本信息

批准号：
01480308
负责人：
YOSHIKAWA Keiji
金额：
$ 4.48万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480308/
关键词：
Severe infection Energy metabolism Toral parenteral Glucose turnover [U- ^<14>C] -Glucose [6- ^3H] -Glucose nutrition Protein turnover [1- ^<14>C] -Leucine 蛋白合成率ロイシン分岐鎖アミノ酸ブドウ糖脂肪酸ラジオアイソト-プ Constant infusion法 constant inf

项目摘要

We studied the effects of glucose administration on whole body glucose kinetics by the primed-constant infusion method of [6- ^3H] - and [U ^<14>C] glucose during total parenteral nutrition (TPN) . Five beagle dogs were given glucose as an energy substrate via TPN route (O-10mg/kg/min) for 72 hours and showed marked increase in the glucose turnover rate (Ra) and oxidation rate (Rox) , while endogenous (hepatic) glucose production rate was significantly suppressed as compared to the controls. In addition, these results suggested the limited capacity of the whole body glucose oxidation. Furthermore, the infection increased both the Ra and the Rox.We also investigated the effects of fat administration on whole body giticose kinetics using the same isotopic dilution method as that described above. Beagle dogs were divided into two groups ; animals that were given long-chain triglyceride (LCT) or those received medium-chain triglyceride (MCT) , in which 40% of no-n-protein calories were given by each fat emulsion and the 60% were supplied by glucose. There were no significant differences in plasma glucose levels and Ra between two groups. Plasma immunoreactive insulin levels were not significantly different between two groups. However, the Rox was significantly higher in dogs that were received MCT-TPN as compared to LCT-TPN group.Finally, we did the preliminary study to determine the whole body protein turnover rate and the leucine oxidation rate using the isotopic tracer method of [1- ^<14>C] -leucine. It has been established by us to determine the plasma [l- ^<14>C] -leucine specific activity that is the major obstacle of this analytical method. We will further study the effects of various energy substrates administrations on protein metabolism during TPN in severely infected patients.

我们通过全肠外营养(TPN)期间[6-^3H]-和[U^14C]葡萄糖的启动恒定输注方法研究了葡萄糖施用对全身葡萄糖动力学的影响。五只比格犬通过 TPN 途径（O-10mg/kg/min）给予葡萄糖作为能量底物 72 小时，与对照组相比，葡萄糖周转率（Ra）和氧化率（Rox）显着增加，而内源（肝脏）葡萄糖生成率显着抑制。此外，这些结果表明全身葡萄糖氧化的能力有限。此外，感染增加了 Ra 和 Rox。我们还使用与上述相同的同位素稀释方法研究了脂肪施用对全身纤维糖动力学的影响。比格犬分为两组；给予长链甘油三酯（LCT）或中链甘油三酯（MCT）的动物，其中40％的非蛋白质热量由每种脂肪乳提供，60％由葡萄糖提供。两组之间的血浆葡萄糖水平和Ra没有显着差异。两组之间血浆免疫反应性胰岛素水平没有显着差异。然而，与LCT-TPN组相比，接受MCT-TPN的狗的Rox显着较高。最后，我们使用[1-^14C]-亮氨酸同位素示踪方法进行了初步研究，以确定全身蛋白质周转率和亮氨酸氧化率。我们已经建立了测定血浆[1-^ 14 C]-亮氨酸比活性的方法，这是该分析方法的主要障碍。我们将进一步研究严重感染患者TPN期间各种能量底物给药对蛋白质代谢的影响。