Generation and development of gastric carcinoma from the view point of distribution and function of nerve fibers in the gastric wall.

从胃壁神经纤维的分布和功能来看胃癌的发生和发展。

• 批准号: 01480321
• 负责人: KAMINISHI Michio
• 金额: $ 0.83万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480321/
• 关键词: Gastric cancer; Intragastric nerve fiber; Gut-brain hormone; Duonenogastric reflux; Atrophic gastritis; PCNA; Gastric stump carcinoma; ニトロソアミン化合物; 細胞回転; 腸上皮化生; 胃粘膜内神経分布; VIP; 慢性胃炎; Brdu

1. We investigated the change of distribution of neuropeptides containing nerve fibers in the gastric wall in rat model of chronic gastritis-intestinal metaplasia by instillation of NaOH in the stomach. S-100 positive nerve fibers showed no remarkable change. GRP containing nerve fibers disappeared just after gastric mucosal injury, but gradually recovered with healing of gastric ulcarative lesions. NPY positive nerve fibers showed a mild change in the muscularis propria.2. The effect of gastric mucosal denervation on carcinogenesis was studied. In a model of gastric carcinoma induced by duodenogastric reflux and administration of carcinogenic agent, the denervated group showed significant increases of gastric carcinoma and adenoma compared to the control group. In a model of gastric stump carcinoma, where no carcinogenic agent was given, denervation of the gastric stump significantly enhanced the development of carcinoma and tumrous lesion. Moreover, the denervated group showed a prog … More ress of atrophic change and a significant increase of labeling index of PCNA in the gastric mucosa.3. The distribution of neuropeptides containing nerve fibers in the resected specimen from gastric cancer patients was investigated. S-100 positive nerve fibers were observed in the submucosa and muscularis mucosae, and also in the deep layr of the mucosa in cases with showing a minor change of the gastric mucosa. NF positive nerve fibers were constantly observed in the submucosa but not in the mucosa. Each of GRP,VIP and NPY positive nerve fibers was not observed, and this phenomenon is assumed to be closely related to severity of gastric mucosal changes.4. Accordingly, it has been clarified that the innervation of the gastric mucosa plays an important role in the development of gastric mucosal changes and gastric tumors. Therefore, the relationship among the intragastric concentration of carcinogenic agents, gastric mucosal changes, distribution of neuropeptide containing nerve fibers and activity of cell proliferation factors should be elucidated. Less

1.用氢氧化钠灌胃的方法，观察了慢性胃炎-肠化生模型大鼠胃壁神经肽分布的变化。S-100阳性神经纤维无明显变化。含GRP神经纤维在胃粘膜损伤后即消失，但随着胃溃疡的愈合而逐渐恢复。结论：1.神经肽Y阳性神经纤维在固有肌有轻度改变。本文研究了胃粘膜去神经在癌变中的作用。在十二指肠胃反流和致癌剂诱导的胃癌模型中，失神经组的胃癌和腺瘤比对照组明显增加。在没有给予致癌剂的残胃癌模型中，去神经支配残胃癌显著促进了癌症和肿瘤病变的发展。此外，去神经组还表现出了一种程序…胃粘膜萎缩性改变加重，增殖细胞核抗原标记指数显著增加。本文对胃癌切除标本中神经肽的分布进行了研究。粘膜下层和粘膜肌层可见S-100阳性神经纤维，胃粘膜轻度改变者粘膜深层也有S-100阳性神经纤维。神经营养因子阳性神经纤维在粘膜下层持续可见，但在粘膜内未见。未见GRP、VIP和NPY阳性神经纤维，推测这一现象与胃粘膜病变的严重程度密切相关。因此，已阐明胃粘膜的神经支配在胃粘膜病变和胃肿瘤的发生发展中起重要作用。因此，应阐明胃内致癌物浓度、胃粘膜变化、神经肽类神经纤维分布和细胞增殖因子活性之间的关系。较少

项目成果

邱明麟,ほか: "慢性胃炎モデルにおけるガストリン細胞とVIP含有神経の分布について" 日本消化器病学会雑誌. 87. (1990)

Chiu Minglin 等人：“慢性胃炎模型中胃泌素细胞和含 VIP 神经的分布”，日本胃肠病学会杂志 87。（1990 年）

上西紀夫,大原 毅,邱 明麟 ほか: "迷走神経切離後の胃癌発生に及ぼす十二指腸液胃内逆流の影響について" 消化器癌の発生と進展. 1. 213-218 (1989)

Norio Uenishi、Takeshi Ohara、Myungrin Chiu 等：“十二指肠液胃内反流对迷走神经切断术后胃癌发生的影响”胃肠癌的发生和进展（1989）。

Kaminishi M,Oohara T,Chiu ML,et al.: "Severe gastric mucosal changes following vagotomy with duodenogastric reflux." J Cli Gastroenterol. 14(suppl). S15-S24 (1992)

Kaminishi M、Oohara T、Chiu ML 等人：“迷走神经切断术伴十二指肠胃反流后胃粘膜发生严重变化。”

上西紀夫,ほか: "胃癌の背景粘膜における消化管ホルモン含有細胞の分布について" 日本消化器病学会雑誌. 88. (1991)

Norio Uenishi 等人：“胃癌背景粘膜中含有胃肠激素的细胞的分布”，日本胃肠病学会杂志 88。（1991）

上西紀夫,ほか: "迷切後の脳腸ホルモン含有神経の分布と内分泌細胞の動態" 消化管ホルモン. (X). (1990)

Norio Uenishi 等人：“迷走神经切断后脑肠激素的分布和内分泌细胞的动态”胃肠激素（X）。