Clonal analysis of gastric mucosa and detection of precancerous lesion using X-chromosome inactivation.

使用 X 染色体失活进行胃粘膜克隆分析和癌前病变检测。

• 批准号: 09671284
• 负责人: KAMINISHI Michio
• 金额: $ 2.18万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671284/
• 关键词: Stomach; Gastric cancer; Clonality; Gastric gland; X-chromosome inactivation; Precancerous lesion; X-chromosome methylation; Intestinal metaplasia; 前癌・病変

We tried to pick up glandular cells under a stereomicroscope. But it was impossible to take off all the interstitial tissues. So we decided to analyze clonality of single glands isolated by EDTA method.As the results, at least, 40% of fundic glands were polyclonal and 7% of pyloric glands were polyclonal. These results were different from results reported before, which all the single glands in gastrointestinal tructs were monoclonal. At least, 50% of single intestinal metaplastic glands were polyclonal, regardless of their histological types or locations.We employed a transgenic mouse line whose clonality can be seen in situ. and analyzed the clonality of gastric mucosa. There were polyclonal glands on the murine stomach. And there were some monoclonal patches on the mucosa. These patches grow larger as the mice grow older. The mice were administered carcinogenen, but the patch did not become larger.

我们试着在体视显微镜下取取腺细胞。但不可能把所有的间质组织都去掉。因此，我们决定对EDTA法分离的单腺体进行克隆性分析。结果表明，至少40%的胃底腺是多克隆的，7%的幽门腺是多克隆的。这些结果不同于以往报道的胃肠道单腺体均为单克隆性的结果。至少，50%的单个肠化生腺是多克隆的，无论其组织类型或位置。我们使用了一种转基因小鼠系，其克隆性可以在原位看到。并对胃粘膜克隆性进行分析。小鼠胃上有多克隆腺。粘膜上有一些单克隆性斑块。随着老鼠年龄的增长，这些斑块会变得更大。这些小鼠被注射了致癌物质，但贴片并没有变大。

项目成果

野村幸世,立道昌幸,上西紀夫: "胃粘膜腸上皮化生の胃粘膜面よりの同定法及びその機序の解明" 消化器癌の発生と進展. 10. 351-356 (1998)

Yukiyo Nomura、Masayuki Tatemichi、Norio Uenishi：“从胃粘膜表面识别胃粘膜肠化生的方法及其机制的阐明”胃肠癌的发生和进展（1998）。

Sachiyo Nomura, Michio Kaminishi, Masayuki Tatemichi: "Detection of single glandular intestinal metaplastic gland from mucosal surface of the stomach" Carcinogenesis of gastrointestinal truct. 9. 259-264 (1997)

Sachiyo Nomura、Michio Kaminishi、Masayuki Tatemichi：“从胃粘膜表面检测单个腺体肠化生腺”胃肠道的癌变。

S Nonwa,M Kaminishi,K Sagiyama,T Oohara,H Esumi: "Clonal analysis of isolated intestinal metaplastic glands of stomach using X linked polynor phism." GUT. 42. 663-668 (1998)

S Nonwa、M Kaminishi、K Sagiyama、T Oohara、H Esumi：“使用 X 连锁多态性对胃分离的肠化生腺进行克隆分析。”