Experimental Research for Pancreas Transplantation

胰腺移植实验研究

• 批准号: 01480326
• 负责人: KURODA Yoshikazu
• 金额: $ 4.16万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480326/
• 关键词: pancreas transplantation; pancreatoesophagostomy; two-layer cold storage method; mechanism of pancreas oxygenation; ATP tissue concentration; pancreatic secretory trypsin inhibitor; rejection; pancreas preservation; perfluorochemical; 96時間膵保存; 膵

1. To minimize technical problems in pancreas transplantation, we developed a new technique in which a pancreas graft was placed in the neck, with exocrine drainage by pancreatic ductesophageal anastomosis in dogs (Transplantation 44, 583, 1987). This time we have made sure that the autograft maintains normal exocrine and endocrine functions during at least 3 years (Ref. 4) and also demonstrated the safety of this method for pancreas allotransplantation (Ref. 7).2. We developed a new two-layer-simple cold storage method using perfluorochemical (PFC) for canine pancreas, preservation (Transplantation 46, 457, 1988, and Ref. 2). Using Euro-Collins' solution (EC) as a preservation solution, we have extended the canine pancreas preservation time up to 72 hours (Ref. 3 and 8). In addition 99 hour preservation of canine pancreas has been achieved by this method using UW solution in place of EC (Ref. 9). We have clarified the mechanism of pancreas oxygenation during preservation of this metho … More d (Ref. 6) and oxygenation of the pancreas allows continued ATP production within the graft and metabolic processes vital to cellular integrity can be maintained, which produces an extended period of preserved pancreatic viability (Ref. 13). We also have found that graft function after transplantation is correlated with ATP tissue concentration, with superior results associated with high ATP level provided by the two layer method (Ref. 11) and the tissue level of ATP at the end of cold preservation by this method will predict the viability of the pancreas graft following transplantation (Ref. 12). To transfer this method to clinical settings, we have clarified that human pancreas is also oxygenated and maintains high ATP tissue concentration during at least 48 hour preservation (Ref. 14).3. We found that serum pancreatic secretory trypsin inhibitor (PSTI) was elevated in the early process of experimental canine pancreas rejection (Transplantation 46, 493, 1988 and Ref. 1) and this elevation is suppressed by immunosuppressive drugs (Ref. 7). In clinical pancreas graft rejection, serum PSTI is also an early sensitive and reliable marker (Ref. 10). Less

1. 为了尽量减少胰腺移植的技术问题，我们开发了一种新的技术，将胰腺移植物放置在颈部，通过胰管食管吻合引流狗的外分泌（transplantation 44,583, 1987）。这一次，我们已经确保自体移植物在至少3年内保持正常的外分泌和内分泌功能（参考文献4），并证明了这种方法用于胰腺异体移植的安全性（参考文献7）。我们开发了一种新的双层简单冷藏方法，使用全氟化学（PFC）来保存犬胰腺（移植46、457、1988和参考文献2）。使用Euro-Collins的溶液（EC）作为保存溶液，我们延长了犬胰腺保存时间至72小时（参考文献3和8）。此外，用UW溶液代替EC，这种方法可以保存犬胰腺99小时（参考文献9）。我们已经阐明了这种方法保存过程中胰腺氧合的机制……More d（参考文献6）和胰腺的氧合允许移植物内继续产生ATP，并且可以维持对细胞完整性至关重要的代谢过程，从而延长了保存胰腺活力的时间（参考文献13）。我们还发现移植后的移植物功能与ATP组织浓度相关，两层法提供的高ATP水平（Ref. 11）和该方法冷保存结束时的组织ATP水平可以预测移植后胰腺移植物的生存能力（Ref. 12）。为了将这种方法应用于临床，我们已经澄清，人类胰腺也是充氧的，并且在至少48小时的保存期间保持高ATP组织浓度（参考文献14）。我们发现血清胰腺分泌性胰蛋白酶抑制剂（PSTI）在实验性犬胰腺排斥反应的早期过程中升高（Transplantation 46,493,1988 and Ref. 1），这种升高被免疫抑制药物抑制（Ref. 7）。在临床胰腺移植排斥反应中，血清PSTI也是早期敏感和可靠的标志物（Ref. 10）。少

项目成果

Y.Kuroda,Y.Suzuki T.Kawamura,H.Fujiwara Y.Fujino,Y.Saitoh: "Longーterm function and histology of segmental pancreatic autografts with pancreatic exocrine diversion to the esophagus in dogs" The Japanese Journal of Surgery. 20. 685-689 (1990)

Y. Kuroda、Y. Suzuki T. Kawamura、H. Fujiwara Y. Fujino、Y. Saitoh：“犬胰腺外分泌转移至食道的节段性胰腺自体移植物的长期功能和组织学”《日本外科杂志》20。 .685-689 (1990)

Y.Kuroda,Y.Suzuki,T.Kawamura,H.Fujiwara,Y.Fujino,Y.Saitoh: "Long-term function and histology of segmental pancreatic autografts with pancreatic exocrine diversion to the esophagus in dogs" THE JAPANESE JOURNAL OF SURGERY.

Y.Kuroda、Y.Suzuki、T.Kawamura、H.Fujiwara、Y.Fujino、Y.Saitoh：“犬胰腺外分泌转移至食管的节段性自体胰腺移植物的长期功能和组织学” 日本外科杂志

Y.Kuroda,Y.Fujino,T.Kawamura,Y.Suzuki,H.Fujiwara,Y.Saitoh: "Mechanism of oxygenation of pancreas during preservation by two layer(Euro-Collins solution/perfluorochemical)cold storage method" TRANSPLANTATION.

Y.Kuroda，Y.Fujino，T.Kawamura，Y.Suzuki，H.Fujiwara，Y.Saitoh：“两层（欧洲柯林斯溶液/全氟化学品）冷藏方法保存期间胰腺的氧合机制”移植。

Y.Kuroda,Y.Fujino,A.Morita,Y.Ku,Y.Saitoh: "Corelation between high adenosine triphoaphate tissue concentration and good posttonsplant outceme of the caniae pancreas gralt after preaervation by two layer cold starage" Transplantation. 52. 989-991 (1991)

Y.Kuroda，Y.Fujino，A.Morita，Y.Ku，Y.Saitoh：“高磷酸腺苷组织浓度与两层冷饲料预保存后的犬胰腺移植后良好结果之间的相关性”移植。

T.Kawamura,Y.Kuroda,Y.Suzuki,H.Fujiwara,K.Yamamoto,Y.Saitoh: "A new simple two-layer(Euro-Collins'stlution/perfluorocheical)cold storage method for pancreas prcservation" Transplant Proc. 21. 1376-1377 (1989)

T.Kawamura，Y.Kuroda，Y.Suzuki，H.Fujiwara，K.Yamamoto，Y.Saitoh：“一种用于胰腺保存的新型简单两层（欧洲柯林斯溶液/全氟化物）冷藏方法”移植过程。