Reserch of Clinical islet transplantation from non-heart beating donor using 2-step two-layer method

2006年3月，在胰岛移植工作组会议上，我所被批准为西部近海和中部地区的胰岛移植中心。我们的胰岛移植计划是这样开始的：我们派出专门的团队从非心脏跳动的捐赠者那里获取器官，并在运输过程中采用双层方法保存人胰腺。我们用Ricordi方法将人胰岛从胰腺中分离出来，并检测胰岛恢复情况。在分离过程和胰岛培养一天后，我们用两层法对人胰岛进行形态和功能评估。当胰岛恢复超过受者体重的5000 IE/kg时，我们对1型糖尿病患者进行胰岛移植。通过动物实验，我们证实并报道了在胰岛分离之前应用两层胰腺保存方法改善了胰岛的恢复和功能。此外，我们还研究了两层法对胰岛cu…的有效性更多的诱惑。上述实验表明了两步双层法在胰岛移植中的重要性。2005年，我们进行了三例人胰岛分离。在三个病例中的一个病例中，我们对一位1型糖尿病患者进行了胰岛移植。供体为32岁女性，热缺血时间6min，冷缺血时间193min，二层法保存时间165min。纯化前回收率为545267 IEQ，纯化后回收率为483545 IEQ。达到了胰岛移植的标准。受者为一名患有不稳定性1型糖尿病的30岁女性。我们可以检测到胰岛移植后受者内源性胰岛素的释放。2004年，我们确认了两步两层法的优越性。2005年，我们提出了从热缺血损伤的胰腺移植胰岛的可能性。临床上，我们报道了一例成功的从年轻、低体重指数、无心脏跳动的身体移植人胰岛的有价值的案例。较少

Our institute was approved as an islet transplantation center of west Kinki and Chugoku area at a meeting of the islet transplant working group in March 2006. We started our islet transplant program as follows.We send our special team for organ procurement from a non-heart-beating donor and preserve a human pancreas by the two-layer method during transportation. We isolate human islets from the pancreas by the Ricordi method and examine islet recovery. We perform morphological and functional assessments of human islets after the isolation process and after one-day culture of islets by the two-layer method. When the islet recover is over 5000 IE/kg of a recipient weight, we perform islet transplantation to a patient of type 1 diabetes.By animal experiments, we certified and reported improvement of islet recovery and function by applying the two-layer method for pancreas preservation prior to islet isolation. And furthermore we investigated usefulness of the two-layer method for islet cu … More lture. The above experiments show importance of the two-step two-layer method for islet transplantation.In 2005 we performed three cases of human islet isolation. From one of the three cases, we performed islet transplantation to a recipient of type 1 diabetes. Donor was 32 year old female, warm ischemia time was 6min, cold ischemic time was 193min, and preservation period by the two-layer method was 165min. Pre purification recovery was 545267 IEQ, and post purification recovery was 483545 IEQ. This was up to the standard of islet transplantation. The recipient was a 30-year old female of unstable type 1 diabetes. We can detect endogenous insulin release in the recipient after islet transplantation. The recipient was dissolved unstableness of blood glucose level.In 2004, we confirmed superiority of the two-step two-layer method. In 2005, we showed the possibility of islet transplantation from the pancreas damaged by warm ischemia. Clinically we reported a valuable case of successful human islet transplantation from a young, low BMI, non-heart-beating cadaver. Less