权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Reserch of Clinical islet transplantation from non-heart beating donor using 2-step two-layer method

两步二层法无心跳供体胰岛移植临床研究

基本信息

批准号：
15209043
负责人：
KURODA Yoshikazu
金额：
$ 30.7万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15209043/
关键词：
islet translation non-heart beating donor warm ischemic time two-layer method islet culture

项目摘要

Our institute was approved as an islet transplantation center of west Kinki and Chugoku area at a meeting of the islet transplant working group in March 2006. We started our islet transplant program as follows.We send our special team for organ procurement from a non-heart-beating donor and preserve a human pancreas by the two-layer method during transportation. We isolate human islets from the pancreas by the Ricordi method and examine islet recovery. We perform morphological and functional assessments of human islets after the isolation process and after one-day culture of islets by the two-layer method. When the islet recover is over 5000 IE/kg of a recipient weight, we perform islet transplantation to a patient of type 1 diabetes.By animal experiments, we certified and reported improvement of islet recovery and function by applying the two-layer method for pancreas preservation prior to islet isolation. And furthermore we investigated usefulness of the two-layer method for islet cu … More lture. The above experiments show importance of the two-step two-layer method for islet transplantation.In 2005 we performed three cases of human islet isolation. From one of the three cases, we performed islet transplantation to a recipient of type 1 diabetes. Donor was 32 year old female, warm ischemia time was 6min, cold ischemic time was 193min, and preservation period by the two-layer method was 165min. Pre purification recovery was 545267 IEQ, and post purification recovery was 483545 IEQ. This was up to the standard of islet transplantation. The recipient was a 30-year old female of unstable type 1 diabetes. We can detect endogenous insulin release in the recipient after islet transplantation. The recipient was dissolved unstableness of blood glucose level.In 2004, we confirmed superiority of the two-step two-layer method. In 2005, we showed the possibility of islet transplantation from the pancreas damaged by warm ischemia. Clinically we reported a valuable case of successful human islet transplantation from a young, low BMI, non-heart-beating cadaver. Less

在2006年3月的岛移植工作组会议上，我所被批准为近畿西部和中国地区的岛移植中心。我们的胰岛移植项目是这样开始的。我们派出专门的团队从一个不跳动的捐赠者那里获取器官，并在运输过程中用双层法保存一个人体胰腺。我们用Ricordi法从胰腺中分离胰岛，并检查胰岛的恢复情况。我们通过两层法对分离过程和胰岛培养一天后的人类胰岛进行形态学和功能评估。当胰岛恢复超过受体体重的5000 IE/kg时，我们对1型糖尿病患者进行胰岛移植。通过动物实验，我们证实并报道了在胰岛分离之前采用双层胰腺保存方法可以改善胰岛的恢复和功能。并进一步探讨了双层法在胰岛细胞培养中的实用性。上述实验表明了两步两层法在胰岛移植中的重要性。2005年，我们进行了3例人胰岛分离。我们对其中一例1型糖尿病患者进行了胰岛移植。供体为32岁女性，热缺血时间6min，冷缺血时间193min，双层法保存时间165min。纯化前回收率为545267 IEQ，纯化后回收率为483545 IEQ。这达到了胰岛移植的标准。接受者是一名患有不稳定型1型糖尿病的30岁女性。我们可以检测胰岛移植后受体体内的内源性胰岛素释放。受体溶解血糖水平不稳定。2004年，我们证实了两步两层法的优越性。2005年，我们展示了从热缺血损伤的胰腺中移植胰岛的可能性。在临床上，我们报告了一例成功的胰岛移植病例，该病例来自一名年轻、低BMI、无心脏跳动的尸体。少

项目成果

期刊论文数量（43）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Influence of Pancreas Preservation on Human Islet Isolation Outcomes: Impact of the Two-Layer Method

DOI：
10.1097/01.tp.0000133515.37892.d5
发表时间：
2004-07
期刊：
Transplantation
影响因子：
6.2
作者：
T. Tsujimura;Y. Kuroda;J. Avila;T. Kin;J. Oberholzer;A. M. Shapiro;J. Lakey
通讯作者：
T. Tsujimura;Y. Kuroda;J. Avila;T. Kin;J. Oberholzer;A. M. Shapiro;J. Lakey

Takada M, Toyama H, Tanaka T, Suzuki Y, Kuroda Y.: "Augmentation of interleukin-10 expression in islets after brain death"Transplant Proc. (in press).

Takada M、Toyama H、Tanaka T、Suzuki Y、Kuroda Y.：“脑死亡后胰岛中白细胞介素 10 表达的增强”移植过程。