Study of orthotopic and auxiliary partial liver grafting from living donor

1)一种辅助性部分肝移植方法的建立在分离出幼猪全肝后，通过台式手术分离左内、外叶，将左叶异位移植于受体肝下部，保留受体肝。移植肝静脉与受体下腔静脉吻合，移植肝门静脉斜向吻合，受体肝门周围血流阻断，使移植肝恢复功能，目前尚无稳定的结果，但部分病例存活不少于5周。2）原位部分肝移植术式的建立（左内叶，外叶）原位移植于受体，其中分离整个肝脏。从供者身上切取部分肝脏并分离受体的整个肝脏需要相当多的技术，但原位部分肝脏移植不需要大量的手术时间。 ...更多信息 从供体猪中获得不少于5天的存活。3）部分肝移植和免疫反应的移植肝大小的检查为了检查受体所需的移植肝大小，检查了移植肝的大小和再生情况，大鼠原位部分肝移植的存活率与全肝移植相似，移植肝重量在5天内恢复到原来的重量手术后。组织学上，术后2天经常观察到肝细胞有丝分裂，但术后5天很少。而同基因20%部分肝移植术不仅术后肝功能障碍加重，生存率明显降低，而且肝ATP再合成减少，内毒素血症增加。以上结果表明，大于30%的部分肝移植能成功地维持受者的生命。在大鼠30%部分肝移植模型中对免疫抑制剂的检查表明，FK 506比CyA对肝功能障碍的责任更小。少

1) Establishment of auxiliary partial liver grafting procedure after isolating the whole liver of a young pig, left internal and external lobes were separated by bench surgery, and the left lobe was heterotopically grafted in the lower part of the recipient's liver, leaving the latter, as it was. Anastomosis of the graft hepatic vein with the recipient's IVC, porto-portal anastomosis by oblique graft portal vein and through blood flow blockage around the recipient hepatic hilum enable us to make the grafted liver functional No stable results have been obtained yet, but we have some cases of not less than 5-week survival.2) Establishment of orthotopic partial liver grafing procedureLeft lobe (left internal lobe, external lobe) of a young pig was orthotopically grafted in the recipient, of which the whole liver was isolated. Harvest of a partial liver from the donor and isolation of the recipient's whole liver require considerable amount of skill, but orthotopic partial liver grafting fr … More om the donor in pigs obtained not less than 5-day survival. Moreover, these grafted livers were confirmed to show regenerative hypermat rophy and good function.3) Examination of the graft size for partial liver grafting and immunological reactionWith the view of examining the size of graft liver necessary for the recipient, the size and the regeneration of the graft liver were examined, using rat orthotopic partiall liver grafting showed approximately similar survival rate to total liver grafting and had grafted liver weight recovered to its original weight 5 days postoperatively. Histologically, hepatocytic karyokinesis was noted frequently 2 days postoperatively, but rarely 5 days postoperatively. On the contrary, isogeneic 20%-partial liver grafting showed not only enhanced postoperative hepatic dysfunction and remarkably lowered survival rate, but also lowered hepatic ATP resynthesis and higher endo-toxihemia compared with the former grafting. The above results indicated that partial liver grafting>30% at least was successful in keeping the recipients life. Examination of iunosuppressants in rat's 30% partial liver grafting model suggested FK506 to be less responsible for hepatic dysfunction than CyA. Less