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Study of Orthotopic Liver Transplantation in Warm Ischemically Damaged Graft-Protective Effects by Antioxidants-

原位肝移植在热缺血损伤中抗氧化剂保护作用的研究-

基本信息

批准号：
61480269
负责人：
DOHI Kiyohiko
金额：
$ 3.01万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

1) The phenomenon of reperfusion injury should occur not only after transient hepatic ischemia, but also during hepatic transplantation. Pretreatment with coenzyme Q_<10>(CoQ<@210)was accompanied by a siqnificant increase in the survival rete (45%) of 30-minute warm ischemically damaged (30-WID) liver grafts compared with 0% in nontreated liver grafts. CoQ<@D210@>D2 pretreatment completely suppressed the marked increase of lipid peroxide and decrease of endogenous CoQ homologues (CoQ<@D29@>D2, Coq<@D210@>D2) 4 hours after grafting of 30-WID liver, resulting in the improvement of enerfy metabolism. This result suggests that exogenous CoQ<@D210@>D2 acts as antioxidant of lipid peroxide. These findings provide that reperfusion damage in rat liver after orthotopic transplantaion is partly due to stimulated lipid peroxidation on biomembranes.2) One of the major problems accompanying liver transplantation is how to evaluate the viability of the grafted tissue at an early stage. The ability t … More o assess immediate graft function would provide results useful in the determination of prognosis. In this study, it was determined that bile flow rates after liver transplantation were correlated with adenosine triphosphate levels and the survival of rats given transplants. Thus it was shown in this experimental transplantation model that the monitoring of bile production after grafting is a useful indicator for assessing the extent of ischemic damage to the liver and for prognosis of the animal.3) In liver transplantation surgery, the cuff technique (non-suture vascular anastomosis) in comparison with hand-suture technique can minimize warm ischemic damage to the liver and hemodynamic diturbance during portal-venous clamping to the recipient, and also avoid hemorrhage due to coagulation abnormalities. Recently, new synthetic absorbable cuff materials (lactic acid-glycolic acid copolymer as a biodegradable material) have been developed in Japan. We attempted the application of this absorbable cuff materials to vascular anastomosis in rat liver grafting. The cuff layer was completely absorbed 5 months after anastomosis and any stenosis and thrombosis did not exit in the anastomosed-vascular lumen. Less

1)再灌注损伤现象不仅发生在短暂性肝缺血后，在肝移植过程中也同样存在。辅酶Q_&lt；10&gt；(@210)可显著提高30分钟热缺血损伤(30-WID)肝移植物存活率(45%)，而未处理肝移植物存活率为0%。Coq&lt；@D210@&gt；D2可完全抑制30周肝移植后4h内脂质过氧化水平的显著升高和内源性辅酶Q同系物(Coq&lt；@D29@&gt；D2，Coq&lt；@D210@&gt；D2)的降低，从而改善能量代谢。这一结果表明，外源Coq&lt；@D210@&Gt；D2具有抗氧化作用。这些结果表明，大鼠原位肝移植后的再灌注损伤部分是由于生物膜上的脂质过氧化作用所致。2)肝移植的主要问题之一是如何在早期评估移植组织的活性。测试…的能力更多地评估即刻移植肾功能将为判断预后提供有用的结果。在这项研究中，确定了肝移植后的胆汁流量与三磷酸腺苷水平和接受肝移植的大鼠的存活率相关。因此，在这个实验性的移植模型中，监测移植后胆汁的产生是评估肝脏缺血损伤程度和判断动物预后的有用指标。3)在肝移植手术中，袖套法(非缝合血管吻合术)与手缝法相比，可以最大限度地减少对肝脏的热缺血损伤和门静脉阻断时的血流动力学障碍，并避免凝血功能异常引起的出血。最近，日本开发了新的合成可吸收袖带材料(乳酸-乙醇酸共聚物作为可生物降解材料)。我们尝试了这种可吸收袖带材料在大鼠肝移植血管吻合中的应用。吻合口5个月后袖套层完全吸收，吻合血管管腔内无狭窄和血栓形成。较少