Study for development of abult partial liver transplantation from living donor

活体部分肝移植的发展研究

• 批准号: 04454318
• 负责人: DOHI Kiyohiko
• 金额: $ 1.28万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454318/
• 关键词: Heterotopic Partial Liver Transplantation; Anti-Adherent Molecule; Endotoxin Shock; 生体部分肝移植; 異所性成人部分肝移植; 部分肝移植; 肝細胞増殖因子; 細胞接着分子

1) Establishment of methods for partial hepatic resection and preservationWe have established a technic of partial (20-50%) hepatic resection without hemorrhage or ischemia in rats and pigs. We have developed "Futhan rines preservation solution", with whchi a rat liver graft can be preserved for 48 hours. It preserved longer than UW solution (24 hours). This solution was like the extra-cellular fluid electrolytic composition which had low potassium level.2) Hepatic cellular growth factor and its application to the hepatic transplantationHepatic cellular growth factors were produced in the pig partial hepatic resection model. As their titers were somewhat unstable, we have not identified a proper growth factor in pig.3) Heterotopic abult partial hepatic transplantationWe established a model of heterotopic abult partial hepatic transplantation in pigs, in which a partial (50%) hepatic graft was planted in the infra-hepatic region of a recipient. This graff enlarged and had an adequate fu … More nction in the recipient recipient occlusion of its hepatic artery and portal vein.4) Production of anti-leukocyte adhesion molecule antibodies and their application to the partial hepatic transplantationProtection of hepatic wam ischemia : Male Wistar rats received partial (70%) hepatic ischemia for 90 min. Five minutes before the ischemia, monoclonal antibody (mAb) against ICAM-1, CD11a, or CD18 was administered intravenously. Then, these mAbs suppressed the infiltation of PMNs, reduced hepatic MDA level, increased ATP resynthesis and significantly increased the survival rate of rats.Endotoxin Shock : Lipopolysaccharide (LPS : 30 mg/kg) was administered to ICR mice intraperitoneally, and 1mg/kg mAb (anti-CD11a, anti-CD11b, anti-CD18, anti-ICAM-1 or anti-LECAM-1) or 30 mg/kg methylprednisolone (MP) was administered intravenously, simultaneously. These treatments significantly increased the survival rate of mice, and mAb(anti-CD18) and MP reduced the hepatic MDA level.These results contribute to the clinical development of the adult partial hepatic transplantation. Less

1)肝部分切除和保存方法的建立我们已经建立了大鼠和猪肝部分（20-50%）切除且无出血或缺血的技术。我们研制了一种大鼠移植肝保存液，可使移植肝保存48小时。它比UW溶液保存时间更长（24小时）。2）肝细胞生长因子及其在肝移植中的应用在猪部分肝切除模型中制备肝细胞生长因子。由于其滴度不稳定，我们还没有确定合适的生长因子在猪体内。3）异位部分肝移植我们建立了一个猪异位部分肝移植模型，其中部分（50%）肝移植物被种植在受体的肝下区域。移植物增大，具有足够的功能。 ...更多信息 4）抗白细胞粘附分子抗体的制备及其在部分肝移植中的应用雄性Wistar大鼠接受部分（70%）肝缺血90分钟，缺血前5分钟，用抗ICAM-1，CD 11 a，或静脉内施用CD 18。结果表明，这些单抗能抑制中性粒细胞的浸润，降低肝脏MDA含量，增加ATP合成，显著提高大鼠的存活率。（LPS：30 mg/kg）腹腔内给予ICR小鼠，（抗-CD 11 a、抗-CD 11b、抗-CD 18、抗-ICAM-1或抗-LECAM-1）或30 mg/kg甲泼尼龙（MP）同时静脉内施用。结果表明，抗CD 18单抗和MP均能显著提高小鼠存活率，降低肝组织MDA水平，为成人部分肝移植的临床应用奠定了基础。少

项目成果

Takashi Maeda: "Effects of Anti-leukocyte-adhesion molecule Antibodies, Nitric Oxide Synthetase Inhibitor, and Methyl-prednisolone on Endotoxin-shock Mice" European Surgical Research. 26(s1). 68- (1994)

Takashi Maeda：“抗白细胞粘附分子抗体、一氧化氮合成酶抑制剂和甲基泼尼松龙对内毒素休克小鼠的影响”欧洲外科研究。

丸林誠二: "肝移植における新しい手術手技:異所性部分肝移植の臨床応用における実験的研究" 日消外会誌. 27(2). 134 (1994)

Seiji Marubayashi：“肝移植新手术技术：异位部分肝移植的临床应用实验研究”《日州外国语杂志》27（2）134（1994）。

福田 康彦: "生体部分肝移植の諸問題" 移植. 26. 171- (1992)

福田康彦：“活体部分肝移植的问题”移植。 26. 171- (1992)

土肥,雪彦: "生体部分肝移植の問題点" 手術. 46(12). 1807-1814 (1992)

Doi，Yukihiko：“活体部分肝移植的问题”46（12）1807-1814（1992）。

Yoshio Oshiro: "Contribution of Neutrophils and Effect of Monoclonal Antibodies to Adhesion Molecules on Ischemia and Reperfusion Injury in Rat Livers" Transplantation Proceedings. 27 (1). 743-744 (1995)

Yoshio Oshiro：“中性粒细胞的贡献和单克隆抗体对粘附分子对大鼠肝脏缺血和再灌注损伤的影响”移植论文集。