Protection of Spinal Cord Injury, Experimental and Clinical Study on Scopic Surgery.

实验性脊髓损伤：1)采用放射免疫法测定大鼠脊髓压迫损伤模型的类二十烷醇生成。OKY-046对TXB_2产生的抑制作用超过80%，但对血管的损伤程度仅降低40%。在豚鼠脊髓受压模型中，TXB_2的产生与受压强度有关，而LTC_4的产生不受受压损伤程度的影响。损伤脊髓中证实的LTC_4的产生提示其与脊髓损伤的继发性疾病有关。2)实验采用ODS大鼠。研究了维生素C对大鼠脊髓缺血相关性压迫损伤的影响。我们研究了血清和脊髓组织中抗坏血酸和α -生育酚的水平，以及脊髓中硫代巴比妥酸（TBA）反应物质的水平。维生素c缺乏组和维生素c补充组血清和脊髓中herol含量明显降低。补充维生素C可明显减轻脊髓损伤所致的运动障碍。脊髓病理检查显示，维生素c补充组脊髓损伤较小，如出血。在脊髓损伤中，维生素C和维生素E可能通过在不同阶段抑制脂质过氧化作用来预防组织损伤。显微手术实验：1)采用Wistar大鼠第5腰椎假关节炎型峡部裂模型，通过组织学研究其骨诱导活性。对照方法为自体松质骨移植、自体松质骨+纤维蛋白胶移植；F组和F组+骨形态发生蛋白；BMP组。虽然并非所有方法均能获得完美的骨融合，但BMP组骨形成优于其他组。因此，显微法移植BMP组具有临床应用的可能性。2)以下研究评估经椎体入路前路减压的疗效。为了确定经椎体入路前路减压后是否会再突出和节段运动退变，我们采用相同的方法切除了双足大鼠的颈椎间盘后部。与相邻椎间盘相比，手术节段椎间盘退变进展明显。实验结果，包括有效的减压和无严重并发症是令人鼓舞的。少

Experimental Spinal Cord Injury : 1) In a rat spinal cord compression injury model, eicosanoids production was determined by radioimmunoassay. The increased TXB_2 production was inhibited more than 80% by OKY-046, but the degree of vascular damage was reduced by only 40%. In a guinea pig spinal cord compression model, TXB_2 production was dependent on the degree of compression strength, while LTC_4 production was not affected by the degree of compression injury. The LTC_4 production confirmed in the injured spinal cords suggested its relationship to secondary disorder in the spinal cord injury. 2) ODS rats were used for experiments. Studies were made on the influence of Vitamin C on the effect of compression injury of the spinal cord associated with ischemia in rats. We investigated the level of ascorbic acid and alpha-Tocopherol in the serum and spinal cord tissue, and the level of thiobarbituric acid (TBA)-reactive substances in the spinal cord. Level of ascorbic acid and alpha-Tocop … More herol in the serum and spinal cord are decresed in the Vitamin C-deficient group and Vitamin C-supplemented group. The motor disturbance induced by spinal cord injury was greatly reduced by Vitamin C supplementation. Pathological examination of the spinal cord showed less damage, such as bleeding, in the Vitamin C-supplemented group. In the spinal cord injury, Vitamin C and Vitamin E may prevent tissue damage by inhibiting lipid peroxidation in the separate phases. Experiment of microscopic surgery : 1) We researched on osteoinductive activity by histological study, using the models of pseudoarhrotic type spondylolysis in the 5th lumbar spine of Wistar rats. Methods of restration were to use transplantation of auto cancellous bone, auto cancellous bone+fibrin glue ; F group and F group+Bone Morphogenetic Protein ; BMP group. Though perfect bone fusion was not obtained through all methods, bone formation through BMP group was superior to other groups. Consiouently it is suggested that there is a possibility of clinical application by using transplantation of BMP group with microscopic method. 2) The efficacy of anterior decompression by the trans-vertebral approach was evaluated in the following studies. To determine whether the reherniation and degeneration of segment motion after an anterior decompression by the trans-vertebral approach, the posterior part of the cervical disc of bipedal rat was extirpated with the same procedure. Progressing disc degeneration was noted at the operated level compared to the adjacent disc. The experimental results, included effective decompression and no serious coplication are encouraging. Less