权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Protection of Spinal Cord Injury, Experimental and Clinical Study on Scopic Surgery.

脊髓损伤的保护、内窥镜手术的实验和临床研究。

基本信息

批准号：
01480369
负责人：
IKATA Takaaki
金额：
$ 4.29万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480369/
关键词：
Spinal cord injury Thromboxane B_2 6-keto-prostaglandin F, alpha Vascular damage Leukotriene C_4 Ascorbic Acid alpha-tocopherol Vitamin E αートロクエロ-ルプロスタグランディン VitE欠乏ラット TBARS トロンボキサンA_2 選択的トロンボキサンA_2合成酵素阻害剤

项目摘要

Experimental Spinal Cord Injury : 1) In a rat spinal cord compression injury model, eicosanoids production was determined by radioimmunoassay. The increased TXB_2 production was inhibited more than 80% by OKY-046, but the degree of vascular damage was reduced by only 40%. In a guinea pig spinal cord compression model, TXB_2 production was dependent on the degree of compression strength, while LTC_4 production was not affected by the degree of compression injury. The LTC_4 production confirmed in the injured spinal cords suggested its relationship to secondary disorder in the spinal cord injury. 2) ODS rats were used for experiments. Studies were made on the influence of Vitamin C on the effect of compression injury of the spinal cord associated with ischemia in rats. We investigated the level of ascorbic acid and alpha-Tocopherol in the serum and spinal cord tissue, and the level of thiobarbituric acid (TBA)-reactive substances in the spinal cord. Level of ascorbic acid and alpha-Tocop … More herol in the serum and spinal cord are decresed in the Vitamin C-deficient group and Vitamin C-supplemented group. The motor disturbance induced by spinal cord injury was greatly reduced by Vitamin C supplementation. Pathological examination of the spinal cord showed less damage, such as bleeding, in the Vitamin C-supplemented group. In the spinal cord injury, Vitamin C and Vitamin E may prevent tissue damage by inhibiting lipid peroxidation in the separate phases. Experiment of microscopic surgery : 1) We researched on osteoinductive activity by histological study, using the models of pseudoarhrotic type spondylolysis in the 5th lumbar spine of Wistar rats. Methods of restration were to use transplantation of auto cancellous bone, auto cancellous bone+fibrin glue ; F group and F group+Bone Morphogenetic Protein ; BMP group. Though perfect bone fusion was not obtained through all methods, bone formation through BMP group was superior to other groups. Consiouently it is suggested that there is a possibility of clinical application by using transplantation of BMP group with microscopic method. 2) The efficacy of anterior decompression by the trans-vertebral approach was evaluated in the following studies. To determine whether the reherniation and degeneration of segment motion after an anterior decompression by the trans-vertebral approach, the posterior part of the cervical disc of bipedal rat was extirpated with the same procedure. Progressing disc degeneration was noted at the operated level compared to the adjacent disc. The experimental results, included effective decompression and no serious coplication are encouraging. Less

实验性脊髓损伤： 1)在大鼠脊髓压缩损伤模型中，通过放射免疫测定法测定类二十烷酸的产生。 OKY-046将增加的TXB_2产量抑制80%以上，但血管损伤程度仅降低40%。在豚鼠脊髓压迫模型中，TXB_2的产生取决于压迫强度的程度，而LTC_4的产生不受压迫损伤程度的影响。受损脊髓中证实的 LTC_4 产生表明其与脊髓损伤的继发性疾病有关。 2)采用ODS大鼠进行实验。研究了维生素 C 对大鼠缺血相关脊髓压迫损伤的影响。我们研究了血清和脊髓组织中抗坏血酸和α-生育酚的水平，以及脊髓中硫代巴比妥酸（TBA）反应物质的水平。缺乏维生素 C 的组和补充维生素 C 的组中，血清和脊髓中的抗坏血酸和 α-Tocop … More 海洛尔水平降低。补充维生素C可大大减少脊髓损伤引起的运动障碍。维生素 C 补充组的脊髓病理检查显示出血等损伤较少。在脊髓损伤中，维生素 C 和维生素 E 可以通过抑制不同阶段的脂质过氧化来防止组织损伤。显微手术实验： 1)我们利用Wistar大鼠第5腰椎假关节型脊椎峡部裂模型，通过组织学研究了骨诱导活性。恢复方法为自体松质骨移植、自体松质骨+纤维蛋白胶移植； F组和F组+骨形态发生蛋白； BMP 组。虽然所有方法均未获得完美的骨融合，但BMP组的骨形成优于其他组。由此可见，用显微镜方法移植BMP组有临床应用的可能性。 2）以下研究评估了经椎体入路前路减压的疗效。为了确定经椎体入路前路减压后是否出现节段运动的再突出和退变，用相同的方法摘除双足大鼠颈椎间盘的后部。与邻近的椎间盘相比，在手术层面注意到进展性椎间盘退变。实验结果令人鼓舞，包括有效减压且未出现严重并发症。较少的