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Experimental studies on the conditioning for the nerve graft or regeneration of the spinal cord.

神经移植或脊髓再生调理的实验研究。

基本信息

批准号：
08457390
负责人：
IKATA Takaaki
金额：
$ 4.67万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457390/
关键词：
spinal cord degeneration injury oxidative stress apoptosis 脊髄損傷一酸化窒素

项目摘要

We investigated the way of prevention of the secondary degenerative changes after mechanical trauma to the spinal cord. We previously demonstrated the implication of the inducible nitric oxide syntase (iNOS) and intercellular adhesion molecule-1 (ICAM-1) of the secondary pathological changes after spinal cord injury, which indicate the important roles of the oxdative stress in the process. We studied the effect of the lecithinized superoxide dismutase (PC-SOD) using rat. The results showed the PC-SOD has positive effects on the preservation of the blood flow, the reduction of the infiltration of the activated neutrophils and the reduction of the eduma in the spinal cord. Clinically, we showed the implication of the leucocytes on the neurological deterioration after spinal cord injury.Correlation between brain is ischemia and delayd nueral death in the brain, which may be induced by nitric oxide derived from iNOS.In the present study, we demonstrated the induction of apoptosis after mechanical trauma to the spinal cord using electrophoresis. Histologically, we demonstrated that the apoptosis can be occurred in neurons.These results indicate that the accumulation of the knowledge on the secondary pathological changes after mechanical trauma to the spinal cord is important for the conditioning for the spinal cord regeneration or graft.

我们研究了预防脊髓机械创伤后继发性退行性改变的方法。我们先前证明了诱导型一氧化氮合酶（iNOS）和细胞间粘附分子-1（ICAM-1）在脊髓损伤后继发性病理变化中的作用，表明氧化应激在此过程中的重要作用。我们利用大鼠研究了卵磷脂化超氧化物歧化酶 (PC-SOD) 的作用。结果表明，PC-SOD对于保存血流、减少活化中性粒细胞的浸润以及减少脊髓水肿具有积极作用。在临床上，我们展示了白细胞对脊髓损伤后神经功能恶化的影响。大脑缺血和延迟性神经死亡之间的相关性可能是由iNOS衍生的一氧化氮引起的。在本研究中，我们通过电泳证明了脊髓机械损伤后细胞凋亡的诱导。在组织学上，我们证明了神经元中可以发生细胞凋亡。这些结果表明，对脊髓机械损伤后继发病理变化的认识的积累对于脊髓再生或移植的调理具有重要意义。