Experimental studies on the conditioning for the nerve graft or regeneration of the spinal cord.

我们探讨了预防脊髓机械性损伤后继发性退行性变的方法。我们已经证实了诱导型一氧化氮合酶(INOS)和细胞间黏附分子-1(ICAM-1)在脊髓损伤后继发性病理变化中的意义，表明氧化应激在这一过程中起着重要作用。我们用大鼠研究了卵磷脂化超氧化物歧化酶(PC-SOD)的作用。结果表明，PC-SOD具有保护脊髓血流量、减少活化中性粒细胞浸润、减轻脊髓组织损伤的作用。在临床上，我们发现白细胞与脊髓损伤后神经功能的恶化有关。脑缺血与延迟性脑死亡之间的关系可能是由诱导性一氧化氮合酶引起的。这些结果表明，脊髓机械性损伤后继发性病理改变的知识积累对于脊髓再生或移植的调理非常重要。

We investigated the way of prevention of the secondary degenerative changes after mechanical trauma to the spinal cord. We previously demonstrated the implication of the inducible nitric oxide syntase (iNOS) and intercellular adhesion molecule-1 (ICAM-1) of the secondary pathological changes after spinal cord injury, which indicate the important roles of the oxdative stress in the process. We studied the effect of the lecithinized superoxide dismutase (PC-SOD) using rat. The results showed the PC-SOD has positive effects on the preservation of the blood flow, the reduction of the infiltration of the activated neutrophils and the reduction of the eduma in the spinal cord. Clinically, we showed the implication of the leucocytes on the neurological deterioration after spinal cord injury.Correlation between brain is ischemia and delayd nueral death in the brain, which may be induced by nitric oxide derived from iNOS.In the present study, we demonstrated the induction of apoptosis after mechanical trauma to the spinal cord using electrophoresis. Histologically, we demonstrated that the apoptosis can be occurred in neurons.These results indicate that the accumulation of the knowledge on the secondary pathological changes after mechanical trauma to the spinal cord is important for the conditioning for the spinal cord regeneration or graft.