Experimental study of factors and conuter measures on spinal cord circulation disturbance

脊髓循环障碍因素及对策的实验研究

• 批准号: 60480340
• 负责人: IKATA Takaaki
• 金额: $ 4.16万
• 依托单位: Tokushima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480340/
• 关键词: Compression injury of spinal cord; Vitamin E; TBA-RS; spinal cord evoked potentisls Syringomyelia; 脊髄空洞症; 髄液脊髄関門

1. Acute spinal cord lesion, (1) Compression injury of rat spinal cord was induced by placing a weight, (a) Spinal cord blood flow produced a hypertensive response after decompression, but restored to preinjury level within one hour in Vitamin E group(group E),while these of control group (group C) lasted by 3 hours. (b) The spinal cord evoked potentisls showed greater recovery in group E than in group C. (c) Histologicaly, the spinal cord showed less damage, such as bleeding or edema, in group E than in group C. (d) The high level of TBA-RS was produced in both groups by compression injury and maintained for 24 hours in group C, but decreased within 1 hour in group E. (2) Acute spinal cord ischemia lesion was made by clipping the thoracic aorta of rats. (a) High TBA-RS level produced by re-circulation was restored to preinjury level within 30 minutes in group E. but in group C lasted by 1 hour. (b) ESP provided more remarkable recovery in group E.2. Chronic spinal cord lesion (1) Syringomyelia of dog spinal cord was induced by subarachnoid injection kaolin; transmedullary passaage of metrizamide from the subarachnoid space to syrinx was shown by delayed CTM, suggesting CSF-spinal cord barrier damage, (2) Tracers such as florescein, Evans blue, ionic lanthanum and HRP was observed to transfer to spinal cord through membranous glia of rat. ( the smaller the molecule, the faster the passage).

1.脊髓急性损伤：（1）用重物压迫大鼠脊髓，（2）减压后脊髓血流量出现高血压反应，维生素E组（E组）1h内恢复至伤前水平，对照组（C组）3 h恢复至伤前水平。(b)脊髓诱发电位恢复E组明显优于C组。(c)脊髓组织学检查，E组出血、水肿等损伤较C组轻。(d)C组在伤后24 h内TBA-RS均升高，而E组在伤后1 h内下降。(2)夹闭大鼠胸主动脉造成急性脊髓缺血损伤。(a)E组再循环引起的高TBA-RS在30分钟内恢复至伤前水平。C组仅持续1小时。(b)E.2组ESP恢复更明显。（2）荧光素、伊文思蓝、离子镧、HRP等示踪剂可通过大鼠膜性胶质细胞进入脊髓。（分子越小，通过越快）。

项目成果

Takaaki Ikata: "Effecto of Vitamin E as A Role of Spinal Cord Protective Drug." International Symposium on Vitamine E.

Takaaki Ikata：“维生素 E 作为脊髓保护药物的作用。”

Takaaki Ikata: "Effect of Vitamin E as A Role of Spinal Cord Protective Drug." Cervical Research Society.

Takaaki Ikata：“维生素 E 作为脊髓保护药物的作用。”

辺見達彦: 臨床整形外科. 21. 407-413 (1986)

边美达彦：临床骨科。21. 407-413 (1986)

Kazuhito Shinohara: "The effect of Vitamine E as a Free Radical Scavenger on Experimental Spinal Cord Injury" International Medical Society of Paraplegia Annual Scientific Meeting in Scandinavia.

Kazuhito Shinohara：“维生素 E 作为自由基清除剂对实验性脊髓损伤的影响”国际截瘫医学会年度科学会议在斯堪的纳维亚半岛举行。

Takaaki Ikata: "Pathogenesis of Experimental Meningitis" The Central Japan Journal of Orthopaedic & Traumatic Surgery.

Takaaki Ikata：“实验性脑膜炎的发病机制” 日本中部骨科杂志