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Experimental study of ear anomaly using whole embryo culture

全胚胎培养耳畸形的实验研究

基本信息

批准号：
01480406
负责人：
JMASUDA Yu
金额：
$ 2.69万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

1) Embryological study of rat ear using whole embryo cultureWistar strain rat embryos wereexplanted at 13 day and cultured in rotating bottles for 36 hours. They were exposed to culture medium by open yolk sac method. Serial sections were made and observed with the light microscope. The middle ear development in cultured embryos were observed nearly as good development as that of 14.5 day embryos in vivo.2) Embryological study of mouse ear using whole embryo cultureICR strain mouse embryos at 11.5 day were cultured for 48 hours. Normal development of the ear were observed to 42 cultured hours not only in the external aspects but also in the histological findings.3) Effect of Vitamin A on the rat ear developmentTwelve day rat embryos were cultured in the medium mixed with Vitamin A for 24 hours in rotating bottles. Cultured embryos were externally normal on the development of craniofacial regions but histological investigations revealed the presence of many eoginophilie granules under the epithelium near by the tubotympanic recess. Transparent electromicroscopy revealed lysozymes to them. Teratological play of them were not clear yet before further investigations.4) Embryological study of mouse ear using whole embryo cultureThirteen day ICR mouse embryos were cut and removed at the ear region and the removed specimens were cultured in rotating bottles with BGJB medium for 48 hours. Cultured specimens were observed with the light microscope and the normally three-dimentional development of middle ear were made sure. Its technical easiness and actually three-dimentional development will be very useful for the study in such a field.

1)大鼠耳部全胚胎培养的胚胎学研究Wistar品系大鼠胚胎移植至第13天，在转瓶中培养36小时。用开放卵黄囊法将其置于培养液中。制作连续切片，用光学显微镜观察。培养胚胎的中耳发育几乎与14.5天的活体胚胎发育一样好。2)小鼠耳的胚胎学研究：11.5天的ICR系小鼠胚胎培养48小时。3)维生素A对大鼠耳发育的影响12日龄大鼠胚胎在含维生素A的培养液中旋转培养24小时。培养的胚胎在颅面部发育方面外观正常，但组织学检查显示，在靠近管鼓室隐窝的上皮下存在许多嗜酸性粒细胞颗粒。透明电子显微镜向它们展示了溶菌酶。4)小鼠耳部全胚胎培养的胚胎学研究：取13天的ICR小鼠胚胎在耳部切开取出，置于含BGJB培养液的旋转瓶中培养48小时。光镜下观察培养标本，确定中耳正常三维发育。它的技术易用性和真正的三维发展将对这一领域的研究非常有用。