Experimental study of ear anomaly using whole embryo culture

1)大鼠耳全胚胎培养的胚胎学研究取Wistar系大鼠13日龄胚胎，转瓶培养36小时。用开放卵黄囊法将其暴露于培养基中。制作连续切片，光镜观察。2）小鼠耳的全胚培养研究取ICR系小鼠11.5日龄的胚胎，培养48小时，观察其中耳的发育情况。3）维生素A对大鼠耳发育的影响将12日龄的大鼠胚胎置于含维生素A的培养液中培养24小时，观察其耳的形态学变化。培养的胚胎是外部正常的颅面区域的发展，但组织学研究显示，存在许多eoginophilie颗粒下的上皮附近的tubotumpanic隐窝。透明电子显微镜显示溶菌酶。4）小鼠耳的胚胎学研究取出生13 d的ICR小鼠胚胎，在耳区切下，置于转瓶中，加入BGJB培养液培养48 h。光镜下观察培养标本，以确定中耳的正常三维发育。其技术上的简单性和实际上的三维开发将是非常有用的研究在这样一个领域。

1) Embryological study of rat ear using whole embryo cultureWistar strain rat embryos wereexplanted at 13 day and cultured in rotating bottles for 36 hours. They were exposed to culture medium by open yolk sac method. Serial sections were made and observed with the light microscope. The middle ear development in cultured embryos were observed nearly as good development as that of 14.5 day embryos in vivo.2) Embryological study of mouse ear using whole embryo cultureICR strain mouse embryos at 11.5 day were cultured for 48 hours. Normal development of the ear were observed to 42 cultured hours not only in the external aspects but also in the histological findings.3) Effect of Vitamin A on the rat ear developmentTwelve day rat embryos were cultured in the medium mixed with Vitamin A for 24 hours in rotating bottles. Cultured embryos were externally normal on the development of craniofacial regions but histological investigations revealed the presence of many eoginophilie granules under the epithelium near by the tubotympanic recess. Transparent electromicroscopy revealed lysozymes to them. Teratological play of them were not clear yet before further investigations.4) Embryological study of mouse ear using whole embryo cultureThirteen day ICR mouse embryos were cut and removed at the ear region and the removed specimens were cultured in rotating bottles with BGJB medium for 48 hours. Cultured specimens were observed with the light microscope and the normally three-dimentional development of middle ear were made sure. Its technical easiness and actually three-dimentional development will be very useful for the study in such a field.