Immunohistochemical study for keratin, involucrin and filagglin in keratinized lesions of oral mucosa.

口腔粘膜角化病变中角蛋白、外皮蛋白和丝丝蛋白的免疫组织化学研究。

基本信息

批准号：
01480426
负责人：
MORI M.
金额：
$ 2.88万
依托单位：
Asahi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480426/
关键词：
Oral mucosa Leucoplakia Squamous cell carcinoma Keratin involucrin filagglin EGF-receptor PCNA インホルクリン DMBA ステロイド Oral mucosa Leukoplakia Squamous cell carcinoma Keratin Involucrin Filagglin

项目摘要

Oral mucosa is divided into 3 parts as masticatory, lining, and specialized mucosa and the distribution of keratin subunits is different in these epithelia. Keratin Nos 8 and 13 were predominantly found in lining epithelial cells and Nos 10, 11, and 18 were in masticatory epithelial cells. Keratin No 19 was found all the basal cells of lining epithelia but seldom in masticatory epithelia. In leukoplakia, filagglin was confined to granular cells and hyperorthokeratinized cells. Involuclin and keratin stainings were similar to the normal skin epithelia lather than oral epithelia. In squamous cell carcinoma, keratins and involuclin were lacked in basal zones of the cancer foci and varied in center tissue. Individual stainability for keratin subunits in carcinoma cells was frequently seen in low grad cancers. EGFreceptor (EGF-r) was found at the plasma membrane of the basal, parabasal, and lower spinous cells in normal and benign oral papillomas. In leukoplakia, hyperkeratinized lesions without elongation of epithelial ridge was usually negative for EGF-r though elongated epithelial ridges was positive for EGF-r. EGF-r positive cells were scattered in SCC. Proliferating cell nuclear antigen (PCNA) was usually seen in the nuclei of the basal and parabasal cells in normal and benign lesions, whereas in the malignant lesions the staining, was spread into upper strata or into malignant foci. PCNA positive cells were characterized by negative for EGF-r.Keratin distributions were also examined in early stages of experimental carcinogens in hamster cheek pouch. KLl staining was reduced in acanthotic lesions. Both KLl and PKKl stainin, were reduced in the lesions with elongation of epithlial ridges.Langerhans cells (LC) which may play an important roles for immunity in the epitheliumi was counted in normal and inframmed hamster cheek pouch with or without cortisone treatment. LC was increased almost double as imframatory change, and decreased about half by cortisone treatment.

口服粘膜分为3个部分，作为咀嚼性，衬里和专门的粘膜，角蛋白亚基的分布在这些上皮中不同。角蛋白编号8和13主要在上皮细胞中发现，而乳房上皮细胞中的10、11和18号。角蛋白第19号被发现上皮衬里的所有基底细胞，但在咀嚼性上皮中很少。在白细胞中，Filagglin仅限于颗粒状细胞和过度低调的细胞。易次熟料和角蛋白染色与正常皮肤上皮泡沫相比，而不是口服上皮。在鳞状细胞癌中，癌症基础区域缺乏角质和易访素，并且中心组织中有所不同。在低毕业癌中经常看到角蛋白细胞中角蛋白亚基的个体可感染性。在正常和良性的口服乳头状瘤中，在基底，副细胞和下棘细胞的质膜上发现EGFRECEPTOR（EGF-R）。在白细胞中，尽管伸长的上皮脊对EGF-R呈阳性，但不伸长上皮脊而没有上皮脊的过度性病变为阴性。 EGF-R阳性细胞分散在SCC中。通常在正常和良性病变的基底和旁巴细胞的核中看到增殖的细胞核抗原（PCNA），而在恶性病变中，染色散布到上层或恶性灶。 PCNA阳性细胞的特征是EGF-R.KERATIN分布在仓鼠脸颊袋中的实验性致癌物的早期阶段还检查了keratin分布。棘皮病变中的KLL染色降低。 kll和pkkl染色蛋白均在上皮脊伸长的病变中均降低。Langerhans细胞（LC），可能在上皮中对免疫的重要作用起着重要作用，并在正常和不接受皮质酮治疗的情况下以正常和不受皮质酮治疗的inframmed仓鼠颊袋进行计数。随着反射性的变化，LC的增加几乎是两倍，可可可的松治疗降低了一半。