Studies on the Kinetics and Spectral Characteristics of Human Cone Photopigments Using Betinal Densitometer

我们用去年建造的四通道麦克斯韦光学系统改进了密度计，做了以下改变。为固定靶提供了四点固定光，而不是以前使用的一点固定光。我们竭尽全力阻挡所有可能的杂散光。然后，利用光电二极管对中性密度滤光片和光楔进行了重新校准。为了控制测试光、参考光和漂白光中的快门，在PC机上开发了软件程序，将测试光和参考光的曝光时间设置为1s。测量了测试光和参照光的反射光强度和暗计数。然后，计算测试光与参考光的光子数之比。这些测量是在光适应期间和之后进行的。此外，还利用心理物理技术测量了暗适应曲线，以比较暗适应的密度学结果。结果表明，视觉色素运动和暗适应的时间进程是不同的机制。

We improved the densitometer with a four channel Maxwellian view optical system which was built last year by making the following changes. A four point fixation light was provided for the fixation target instead of the one point fixation light which was previously used. We made every effort to blockall possible stray light. Then, neutral density filters and optical wedges were recalibrated by using a photo-diode. In order to control the shutters in the test light, the reference light and the bleaching light, software programs were developed on the personal computer.In this experiment we set the exposure time at 1sec for the test and the reference lights. The reflection light intensities for the test light and the reference light and the dark count were measured. Then, the ratio of the photon number for the test and the reference lights was calculated. The measurements were taken both during and after light adaptation. Furthermore, the dark adaptation curves were measured with the psychophysical technique in order to compare the densitometric results.As a result, it was suggested that the visual pigment kinetiks and the time course of dark adaptation are different mechanisms.