Effects of the Periodontal Implant Materials on the Early Stage of Mineralization of Osteoblast-LIKE CELLS

为了了解人工骨植入材料的确切作用，我们进行了体外和体内试验系统的评估。本研究采用集落分离法从小鼠颅骨分离成骨细胞。这些细胞具有成骨功能。为了评价种植材料在成骨细胞中的生物活性和钙化情况，将细胞和种植材料培养21天。培养21天后，羟基磷灰石（HAP）和真骨陶瓷（TBC：牛烧结骨）对这些细胞显示出生物亲和力。但TBC周围新钙化的证据比其他材料多。在本研究的基础上，我们研究了胶原蛋白涂层材料对促进修复和早期钙化的额外作用。我们将TBC和胶原包被的TBC（C-TBC）培养21天。从这些结果来看，用胶原蛋白涂覆材料，成骨细胞表现出更好的修复和早期钙化。在植入人工植入材料后的实验性骨缺损中，在组织学上观察到新骨形成，但这种新形成的骨的成熟程度不能从这种观察中确定。因此，测量钙和磷的密度可用于检查骨形成和种植体材料周围。在三个实验中，我们校准了一个计算机辅助微量分析仪（CMA）来测量钙和磷。该装置由电子探针显微分析仪（EPMA）和计算机图像分析仪组成。术后分别用HAP、TBC或C-TBC填充缺损。对照侧未进行植入。1个月和3个月后，处死两只犬，这些结果表明，可以确定其他组织与植入材料的距离。在三个月的样本中，CMA测量显示TBC和C-TBC植入物周围区域具有高密度的Ca和P。

We have estimated in vitro and in vivo assay system to know the precise effects of artificial bone implant materials. In this study, we isolated osteoblastic cell from mouse calvaria using colony separation. These cells ratain osteoblastic functions. To estimate the biological activity and calcification of implant materials in osteoblastic cells, cells and implant materials were cultured for 21days. After 21days cultivation, hydroxyapatite (HAP) and True Bone Ceramic (TBC : bovine sintered bone) showed the biological affinity on these cells. However the around of TBC showed newly calcified evidence more than other materials. Addition of this study, we examined about the additional effect of promoting the mending and early calcification by coating the materials with collagen. We cultivatied TBC and collagen-coated TBC (C-TBC) for 21 days. From these results, coating the materials with collagen, the osteoblastic cells show better mending and early calcification. New bone formation was observed histologically in experimental osseous defects after implantation of artificial implant material, but the degree of maturation of this newly formed bone could not be determined from such observation. Therefore measurement of the density of Ca and P can be used to examine bone formation and around implant materials. In three experiments, we calibrated a computer-aided micro analyzer (CMA) to measure Ca and P. This devise is composed of an electron probe microanalyzer (EPMA) and a computed image analyzer. After the operation the defects were filled with HAP, TBC or C-TBC. No implantation was performed the control side. After one month and three month, the two dogs were sacrificed and these results show that, it is possible to determine the distance from implant materials of other tis sue. In the three months specimens, CMA measurement showed the area surrounding the TBC and C-TBC implants to have a high density of Ca and P.