Affinity of several GTR membrane in human osteoblast-like cells

生物材料在组织替代中的临床使用和应用的增加导致了一种新的牙周治疗的发展，并探索了GTR（引导组织再生）膜与周围骨组织和结缔组织整合的生物学。在牙周病学中，整合过程被认为对于在骨、结缔组织和人工膜之间建立紧密和稳定的连接是至关重要的（Millipore（）SY.（），Gore-Tex（）SY. encircledR.〔)).在这项研究中，MG 63从成骨细胞系来源于人骨肉瘤培养与或无几个膜。在3天至6天的培养时间期间，进行形态学、细胞外基质（纤连蛋白、I型胶原和III型胶原）的生物合成和骨钙素合成作为矿化功能的检查。成骨细胞与几种人工膜粘附过程中形成的接触部位的性质 ...更多信息 通过ACAS 570激光细胞计数器测量单个标本的平均荧光来研究ne。激光细胞仪显示成骨细胞牢固地粘附在几种人工膜表面。在3天的粘附过程中观察到纤维连接蛋白使成骨细胞牢固地粘附在几种人工膜表面上。在I型和III型胶原上，细胞在接下来的3天内分泌纤连蛋白后变圆。此外，细胞外基质的合成和骨钙素的分泌，矿化后，在有或无膜的文化没有增强。在胶原和纤维连接蛋白合成过程中观察到的R-G-D模式可能是细胞外基质内容物形成的证据。（）和Gore-Tex（）SY. encircledR.（）+膜可能具有良好的生物亲和性，但不刺激成骨细胞产生细胞外基质和骨细胞素。少

Increase clinical usage and application of biomaterials in tissue substitution have led to the development of a new periodontal therapy and explores the biology involved in the integration of GTR(guided tissue regeneration) membranes into the surrounding bone tissue and conective tissue. In periodontics, the process of integration is considered to be of pivotal importance for establishing an intimate and stable connection between bone, conective tissue and artificial membrane (Millipore(〕SY.encircledR.〔), Gore-Tex(〕SY.encircledR.〔)). In this study, MG63 from an osteoblastic cell line derived from human osteosarcoma were cultured with or without several membrane. Examination for morphology, biosynthesis of extracellular matrix (fibronectin, type I collagen and type III collagen) and osteocalcin synthesis as a function mineralization during culture times of 3 days to 6 days, were conducted. The nature of contact sites formed during the adhesion of osteoblasts to several artificial membra … More ne was investigated by the ACAS 570, laser cytometer for measuring average fluorescence on individual specimens. The laser cytometer revealed that osteoblasts adhere securely to the several artificial membrane surface. The fibronectin caused the osteoblasts to adhere securely to the several artificial membrane surface were observed during 3 days of adhesion. On both type of type I and III collagen, the cells became round after secretion of fibronectin during the next 3 days. In addition, the synthesis of extracellular matrix and secretion of osteocalcin, subsequent to mineralization, were not enhanced in cultures both with or without membranes. The R-G-D patterns observed during collagen and fibronectin synthesis may be evidence of the formation of extracellular matrix contents.These results suggest that Millipore(〕SY.encircledR.〔) and Gore-Tex(〕SY.encircledR.〔)+ membrane may have well biological affinity but not stimulate extracellular matrix and osteoclacin production from osteoblastic cells. Less