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Fundamental of optogalvanic laser spectroscopy based on relaxation oscilator detection and its applications.

基于弛豫振荡器检测的光电激光光谱学基础及其应用。

基本信息

批准号：
02452087
负责人：
FUJII Kan-ichi
金额：
$ 4.22万
依托单位：
Ibaraki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

This research subject has been carried out for two years. The investigation contains two aspects ; one of them is further research of optogalvanic spectroscopy itself which was newly developed with an intent of getting higher sensitivity of detecting optogalvanic effect. The other is its application to cell biology.At the part of fundamental investigation, the measurement of optogalvanic signal targeted to the negative glow plasma was expanded to all plasma in a glow discharge like the positive column, Faraday dark space, anode glow, and the cathode dark space. By this means, a high quality measurement of plasma properties became hopeful. During this experiment, unusual optogalvanic signal was detected in the cathode dark space ; i. e., the polarity of the signal was reversed. So, experiments have been still continuing in order to confirm its reproducibility of the new result.At the part of application, the investigation for sorting cancer cell by means of autofluorescence which will be emitted from each cancer cell by the irradiation of laser beam of suitable wavelength was started. This method is the one included in the Raman spectroscopy. The authors started to detect some Stokes line from a mineral such as AgCl particle, and biomaterials like milk, vegetable juice as well. Our final aim is to detect an anti-Stokes line and so, the experiments are being powerfully continued.

本研究课题已进行了两年。研究内容包括两个方面：一方面是对光电流光谱学本身的进一步研究，这是为了获得更高的检测光电流效应的灵敏度而发展起来的。在基础研究部分，将针对负辉光等离子体的光电流信号测量扩展到辉光放电中的所有等离子体，如正柱、法拉第暗区、阳极辉光和阴极暗区。通过这种方法，等离子体特性的高质量测量成为有希望的。在实验过程中，在阴极暗区检测到异常的光电流信号;例如，信号的极性被反转。因此，为了确认新结果的再现性，还在继续进行实验。在应用部分，开始了通过照射适当波长的激光束从每个癌细胞发出的自体荧光来分选癌细胞的研究。该方法是包括在拉曼光谱中的方法。作者从AgCl颗粒等矿物和牛奶、蔬菜汁等生物材料开始探测斯托克斯线。我们的最终目标是检测反斯托克斯线，因此，实验正在有力地继续进行。