Fundamental and applied research in microbial glutathione metabolizing enzymes and their genes
微生物谷胱甘肽代谢酶及其基因的基础与应用研究
基本信息
- 批准号:02453129
- 负责人:
- 金额:$ 4.1万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1990
- 资助国家:日本
- 起止时间:1990 至 1991
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
(1) gamma-Glutamyltaranspeptidase (GGT) gene (ggt) was self-cloned in GGT-less E. coli mutant and this cloned strain showed low-temperature dependent expression of GGT as a wild strain. The results from Western and Northern blotting indicated that the expression controlled not at the processing but at the transcriptional step.(2) The plasmid carrying ggt was transduced into a tola strain of E. coli. The transformant excreted GGT and the GGT was purified by a simple two-step method.(3) The conditions were investigated for the preparation of crystals of GGT suitable for the x-ray analysis and the conditions to obtain large crystals were established.(4) The mutation of Arg 571 to Ala in GGT caused accumulation of inactive pro-GGT in the periplasmic space of the host cell.(5) GILitathione S-transferase (GST) cDNA from a yeast Issatchenkia orientalis was cloned into an E. coli strain and this transformant highly expressed GST. The nucleotide sequence of GST CDNA was determined and the amino acid sequence was deduced. The inducible formation of GST was elucidated to be controlled at the transcriptional step.(6) The glutathione-conjugate was found to be metabolized to eysteine-conjugate by the action of carboxypeptidase-like enzyme in I. orientalis cells.(7) GST cDNA was subcloned to a shuttle-vector and the reconstituted plasmid was transduced into Saccharomyces cerevisiae cells. The transformant showed 15 times higher GST activity than the host cells.(8) The localization of GST at the surface of I. orientalis cells were indicated by cell-fractionation and immune-electromicroscope analysis.
(1)将γ-谷氨酰转氨肽酶(GGT)基因(ggt)自克隆到无GGT的大肠杆菌(E.大肠杆菌突变株和该克隆菌株表现出低温依赖性的GGT表达的野生型菌株。Western和北方印迹分析结果表明,该基因的表达控制在转录水平,而不是加工水平。(2)将携带ggt基因的质粒转化大肠杆菌托拉菌株。杆菌通过简单的两步法纯化了γ-谷氨酰转肽酶。(3)研究了适合于X射线分析的GGT晶体的制备条件,并建立了获得大晶体的条件。(4)GGT中Arg 571突变为Ala导致无活性的pro-GGT在宿主细胞的周质空间中积累。(5)从东方伊萨酵母(Issatchenkia orientalis)中克隆了谷胱甘肽S-转移酶(GST)cDNA,并将其克隆到E.大肠杆菌中表达GST。测定了GST cDNA的核苷酸序列,并推导了其氨基酸序列。GST的诱导形成被阐明为在转录步骤中被控制。(6)谷胱甘肽结合物在I.东方细胞(7)将GST cDNA亚克隆到穿梭载体中,并将重组质粒转导到酿酒酵母细胞中。GST活性是宿主细胞的15倍。(8)GST在I.通过细胞分级分离和免疫电镜分析来指示orientalis细胞。
项目成果
期刊论文数量(38)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Jaime Obien Claudio, Hideyuki Suzuki, Hidehiko Kumagai and Tatsurokuro Tochikura: "Excretion and rapid purification of cloned gamma-glutamyltranspeptidase from Escherichia coli K-12." Annual Reports of International Center of Cooperative Research in Biote
Jaime Obien Claudio、Hideyuki Suzuki、Hidehiko Kumagai 和 Tatsurokuro Tochikura:“从大肠杆菌 K-12 中分泌和快速纯化克隆的 γ-谷氨酰转肽酶。”
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玉置 尚徳: "Detoxification Metabolism of oーDinitroーbenzene by Yeast Issatchenkia orientalis." Agric.Biol.Chem.
Naonori Tamaki:“东方伊萨琴氏菌对邻二硝基苯的解毒代谢。”
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- 影响因子:0
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玉置 尚徳: "Nucleotide Sequence of Yeast Glutathione SーTransferase Yー2 cDNA."
Naonori Tamaki:“酵母谷胱甘肽 S-转移酶 Y-2 cDNA 的核苷酸序列。”
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- 影响因子:0
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Hidehiko Kumagai, Hideyuki Suzuki, Takashi Echigo and Tatsurokuro Tochikura: "Syntheses of gamma-glutamyl peptides by gamma-glutamyltranspeptidase from E. coli." Annals of New York Academy of Sciences. 613. 647-651 (1990)
Hidehiko Kumagai、Hideyuki Suzuki、Takashi Echigo 和 Tatsurokuro Tochikura:“利用大肠杆菌的 γ-谷氨酰转肽酶合成 γ-谷氨酰肽。”
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- 影响因子:0
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Hisanori Tamaki, Hidehiko Kumagai and Tatsurokuro Tochikura: "Nucleotide sequence of the yeast glutathione S-transferase cDNA." Biochemica et Biophysica Acta. 1089. 276-279 (1991)
Hisanori Tamaki、Hidehiko Kumagai 和 Tatsurokuro Tochikura:“酵母谷胱甘肽 S-转移酶 cDNA 的核苷酸序列。”
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- 影响因子:0
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KUMAGAI Hidehiko其他文献
KUMAGAI Hidehiko的其他文献
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{{ truncateString('KUMAGAI Hidehiko', 18)}}的其他基金
Studies on microbial aromatic L-amino acid decarboxylase
微生物芳香族L-氨基酸脱羧酶的研究
- 批准号:
21380062 - 财政年份:2009
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Conversion of inverting glycosidases to glycosynthasesand its application to regio- and stereospecific synthesis ofoligosaccharide
反转糖苷酶向糖合酶的转化及其在低聚糖区域和立体特异性合成中的应用
- 批准号:
19380062 - 财政年份:2007
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular and cellular research on aromatic amino acids production, by microorganisms.
微生物生产芳香氨基酸的分子和细胞研究。
- 批准号:
14360056 - 财政年份:2002
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular and cellular studies on biofunctional proteins from microorganisms.
微生物生物功能蛋白的分子和细胞研究。
- 批准号:
10306007 - 财政年份:1998
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (A).
Preparation of Monoclonal Antibody against Mutagenic Sugar Chain Which Specifically Occurs in Hepatoma Cells and Its Evaluation for Diagnostics
肝癌细胞特异糖链突变单克隆抗体的制备及其诊断评价
- 批准号:
10556079 - 财政年份:1998
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of new food materials using specific catalitical activities of microbial enzymes
利用微生物酶的特定催化活性开发新食品材料
- 批准号:
07556023 - 财政年份:1995
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Studies on structure and function of amine and amino acid related enzymes from microbiology.
从微生物学角度研究胺和氨基酸相关酶的结构和功能。
- 批准号:
06453168 - 财政年份:1994
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular Mechanism of Defense against Stress and Metabolic Regulation by Glutathione and Its Metabolites
谷胱甘肽及其代谢物抗应激及代谢调节的分子机制
- 批准号:
02304035 - 财政年份:1990
- 资助金额:
$ 4.1万 - 项目类别:
Grant-in-Aid for Co-operative Research (A)
相似海外基金
Analysis of genetic polymorphisms of bitter taste receptor TAS2R38 and glutathione-S-transferase (GST), and its relationship with eating habits in Japanese megabank database
日本大型银行数据库苦味受体TAS2R38和谷胱甘肽-S-转移酶(GST)基因多态性分析及其与饮食习惯的关系
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Characterization of human glutathione S-transferase P1-1-catalyzed glutathionylation of proteins: as a clue to understand the inter-individual difference in drug-induced toxicity
人谷胱甘肽 S-转移酶 P1-1 催化的蛋白质谷胱甘肽化的表征:作为了解药物引起的毒性个体间差异的线索
- 批准号:
19K16348 - 财政年份:2019
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Grant-in-Aid for Early-Career Scientists
Investigation of differing dynamics during catalysis by Glutathione-S-Transferase
谷胱甘肽-S-转移酶催化过程中不同动力学的研究
- 批准号:
524345-2018 - 财政年份:2018
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University Undergraduate Student Research Awards
A structure biological approach toward the identification of an endogenous substrate of the insect glutathione S-transferase Nobo
鉴定昆虫谷胱甘肽 S-转移酶 Nobo 内源底物的结构生物学方法
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18K19163 - 财政年份:2018
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Cancer risk assessment study on gene polymorphism of bitter taste receptor TAS2R38 and glutathione-S-transferase by analyzing ToMMo data
通过分析ToMMo数据进行苦味受体TAS2R38和谷胱甘肽-S-转移酶基因多态性的癌症风险评估研究
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18K18442 - 财政年份:2018
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$ 4.1万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Thiol switches controlled by the glutathione-S-transferase GDAP1
由谷胱甘肽-S-转移酶 GDAP1 控制的硫醇开关
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386417025 - 财政年份:2017
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Priority Programmes
The Physiological Role of Glutathione-S-Transferase in the Intracellular Storage and Transport of Nitric Oxide and its Biomedical Effects
谷胱甘肽-S-转移酶在一氧化氮的细胞内储存和运输中的生理作用及其生物医学作用
- 批准号:
nhmrc : 1062026 - 财政年份:2014
- 资助金额:
$ 4.1万 - 项目类别:
Project Grants
Role of Glutathione S-Transferase P in Heart Failure
谷胱甘肽 S-转移酶 P 在心力衰竭中的作用
- 批准号:
8214662 - 财政年份:2010
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$ 4.1万 - 项目类别:
Role of Glutathione S-Transferase P in Heart Failure
谷胱甘肽 S-转移酶 P 在心力衰竭中的作用
- 批准号:
8423348 - 财政年份:2010
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$ 4.1万 - 项目类别:
Glutathione S-Transferase Pi in Age-Related Macular Degeneration
谷胱甘肽 S-转移酶 Pi 在年龄相关性黄斑变性中的作用
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8305622 - 财政年份:2010
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