Studies on microbial aromatic L-amino acid decarboxylase

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Aromatic amino acid decarboxylase is a key enzyme for synthesis of various biogenic amines and their derivatives. In this study, we isolated the genes encoding the enzyme from various bacteria, and carried out enzymatic analyses for their products. We also succeeded in constructing a bacterial platform for synthesis of the plant alkaloid using a recombinant E. coli strain. In addition, fermented food microbiota was investigated by using pyrosequencing technique to evaluate the existing ratio of lactic acid bacteria with abilities to produce biogenic amines.