Dynamic aspects of microbody transition in pumpkin cotyledons.

南瓜子叶微体转变的动态方面。

• 批准号: 02454012
• 负责人: NISHIMURA Mikio
• 金额: $ 4.03万
• 依托单位: Okazaki National Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454012/
• 关键词: Microbody transformation; Glyoxysomes; Leaf peroxisomes; Degradation of glyoxysomal enzymes; cDNA cloning; Senescence; Reversible transformation; マイクロボディ; 機能転換; グリオキシゾ-ム; 緑葉パ-オキシゾ-ム; Malate synthase; Isocitrate lyase; カボチャ; リンゴ酸シンタ-ゼ; リンゴ酸脱

There occurs functional transformation of microbodies from glyoxysomes to leaf peroxisomes during greening of pumpkin cotyledons. In order to clarify the regulatory mechanisms underlying the microbody transformation, glyoxysomal and leaf peroxisomal enzymes were purified and their specific antibodies and cDNA were prepared. By the analysis using these antibodies and cDNA clones, it was found that increase of leaf peroxisomal enzymes is due to increase in level of their mRNA and decrease in glyoxysomal enzymes is came not only by decrease in level of their mRNA but also by induction of specific degradation system for glyoxysomal enzymes in microbodies during microbody transformation.Reversible transformation of microbodies from leaf peroxisomes to glyoxysomes was found to occur in the cotyledons during the senescence. The development of glyoxysomal enzymes was also observed in petals of the pumpkin flower during senescence, indicating that glyoxysomes play an important roles in senesceing process.

在南瓜子叶变绿过程中，微体的功能发生了从乙醛酸体向叶过氧化物酶体的转化。为了阐明微体转化的调控机制，我们纯化了乙二醛酶体和叶过氧化物酶体酶，并制备了它们的特异性抗体和cDNA。通过使用这些抗体和cDNA克隆的分析，结果表明，在微体转化过程中，过氧化物酶体酶活性的增加是由于其mRNA水平的增加，而乙醛酶体酶活性的降低不仅是由于其mRNA水平的降低，而且是由于微体中乙醛酶体酶特异性降解系统的诱导，微体从过氧化物酶体到乙醛酶体的转化是可逆的在子叶衰老过程中。在南瓜花衰老过程中，还观察到了乙醛酸酶体的发育，表明乙醛酸酶体在衰老过程中起着重要作用。

项目成果

L. De Bellis and M. Nishimura: "Development of enzymes of the glyoxylate cycle during senescence of the pumpkin cotyledons." Plant Cell Physiology. 32. 555-561 (1991)

L. De Bellis 和 M. Nishimura：“南瓜子叶衰老过程中乙醛酸循环酶的发育。”

Mikio Nishimura: "Intracellular protein trafficking in oil seeds." “Molecular Approaches to Compartmentation and Metabolic Regulation". (1991)

Mikio Nishimura：“油料种子中的细胞内蛋白质运输。”“区室划分和代谢调节的分子方法”（1991）。

Hitoshi Mori: "Pumpkin malate synthase:Cloning and sequencing of the cDNA,and Northern blot analysis." Eur.J.Biochem.,. (1991)

Hitoshi Mori：“南瓜苹果酸合酶：cDNA 的克隆和测序，以及 Northern 印迹分析。”

H.Mori: "Pumpkin malate synthase.Cloning and sequencing of the cDNA and Northern blot analysis." Eur.J.Biochemistry. 197. 331-336 (1991)

H.Mori：“南瓜苹果酸合酶。cDNA 的克隆和测序以及 Northern 印迹分析。”

L.De Bellis: "Glyoxylate cycle enzymes in peroxisomes isolated from petals of pumpkin during senescence." Plant Cell Physiology. 32. 1227-1235 (1991)

L.De Bellis：“在衰老过程中从南瓜花瓣中分离出过氧化物酶体中的乙醛酸循环酶。”